ABSTRACT
Ischemic preconditioning (IPC) has been an excellent strategy for enhancing sports performance recovery, although there is still no consensus on the ideal protocol. Thus, this study aimed to evaluate the effects of IPC with different cuff pressures (low pressure, medium pressure, and high pressure) on the attenuation of neuromuscular fatigue after an isometric test protocol. And to verify whether this improvement was related to muscle oxygen saturation during the test protocol. Thirty males (18-35 years old) with experience in resistance training were allocated to three different groups: low pressure (20 mmHg), medium pressure (100 mmHg), and high pressure (190 mmHg). The individual occlusion pressure of each participant was identified using ultrasound. Each participant performed two test protocols (8 maximal isometric contractions lasting 20-s with a 10-s rest interval) in an extension chair; after the first test protocol, the participant received the IPC intervention with a low, medium, or high cuff pressure or received the noncuff intervention (randomized order). Only the medium-pressure group showed a smaller decrease in mean force change compared to the no-cuff condition (-4.40% vs. -13.10%, p=0.01, respectively), and the low- and high-pressure groups did not exhibit significant pressure differences (IPC vs. noncuff: -8.40% vs. -13.10%, p=0.11 and -9.10% vs. -14.70%, p=0.12, respectively). Muscle oxygen saturation across test protocols showed no significant differences in all IPC conditions (p>0.05). Although, IPC with medium pressure was effective at optimizing the recovery of neuromuscular performance, this improvement is not related to an increase in muscle oxygen saturation during exercise.
ABSTRACT
There has been an increase in the incidence of anal cancer in the past two decades, with squamous cell carcinoma (SCC) being the most frequent histological type identified. Among the risk factors, high-risk human papillomavirus (HPV) infection is the most pervasive. Raf kinase inhibitor protein (RKIP) is expressed in a number of normal human tissues and previous studies have demonstrated the prognostic value of the loss of RKIP expression in several gastrointestinal tumors. Therefore, the present study aimed to evaluate the clinical implications of RKIP expression in a series of neoplastic lesions of the anal canal. The resected tumors of 48 patients [8 high-grade intraepithelial lesions (HSILs), 14 adenocarcinomas and 26 squamous cell carcinomas (SCCs)] were immunohistochemically evaluated for RKIP expression, and the results were correlated with clinicopathological data. The results identified a decreased 5-year overall survival rate in patients with adenocarcinoma (40.8%) compared with patients with SCC (76.7%), and a decreased 5-year disease-free survival rate in patients at clinical stages III/IV (37.3 vs. 62.5 and 82.6% for clinical stages 0 and I/II, respectively). Low RKIP expression was revealed in 62.5% of HSILs, 88.5% of SCCs and 100.0% of the adenocarcinomas. High RKIP expression was associated with patient ethnicity (37.5% in non-Caucasians vs. 7.5% in Caucasians) and patient age (33.3% in younger patients vs. 0.0% in older patients). Finally, high RKIP expression was correlated with HPV16 infection status (40% in HPV- vs. 5.3% in HPV+ patients). A correlation was identified between high RKIP expression and lesions with a generally improved prognosis, such as those diagnosed in younger patients, in situ lesions and lesions of lower clinical grades; there was also a negative correlation between high RKIP expression and HPV16 positivity in patients.
