ABSTRACT
The role of caffeinated beverages on efficiency of acute inflammatory responses is not yet fully understood. This study analyzed the effect of five hot water extracts, coffee (CO), black/green tea (BT/GT), yerba mate (YM), and guarana (GU) on inflammatory modulation of non-activated human peripheral blood mononuclear cells (PBMCs), yeast-activated human neutrophils, and granulocytic coelomocytes from Eisenia fetida earthworm. Based on preliminary tests, a concentration of 10⯵g/mL was chosen for subsequent assays, as at this concentration, the extracts exhibited antioxidant, genoprotective, and non-cytotoxic properties. Immunoassays using 24-h PBMC supernatant showed that all extracts decreased levels of pro-inflammatory cytokines (IL-1ß, IL-6, TNF-α, and IFN-γ), and increased levels of the anti-inflammatory cytokine, IL-10. Further, these extracts induced overexpression of cytokine genes in 24-h cultures. These results suggest that an increase in the levels of mRNAs and/or inactive cytokines in the cytoplasm improves the "immune cytokine response. Analysis of the yeast encapsulation processes, and production of human neutrophils and coelomocyte extracellular DNA traps suggests that extracts also improve the immune response in humans and earthworms. However, for E. fetida, the intensity of these results varied from extract. Overall, our results suggest that caffeinated beverages may improve an organism's efficiency against acute inflammatory processes.
Subject(s)
Beverages , Caffeine/pharmacology , Inflammation/prevention & control , Leukocytes, Mononuclear/drug effects , Animals , Cytokines/immunology , Humans , Inflammation Mediators/immunology , Leukocytes, Mononuclear/immunology , OligochaetaABSTRACT
Mucocele do apêndice é uma entidade rara que ocorre devido à obstruçäo do lúmen ou como seqüela de neoplasias malignas ou benignas desse órgäo. Os autores relatam um caso de mucocele gigante do apêndice em uma mulher de 44 anos cujo quadro de apresentaçäo foi de abdome agudo inflamatório. Säo discutidos aspectos clínicos, anatomopatológicos e terapêuticos da doença
