ABSTRACT
In this report, we describe the identification of HLA-A*1112, a novel HLA-A*11 allele found in two Italian families. The new allele was detected during routine HLA typing by a polymerase chain reaction sequence-specific primer and was confirmed by high-resolution sequencing-based typing. The nucleotide sequences of HLA-A*1112 exons 2 and 3 are identical to HLA-A*11011 except for a single nucleotide substitution in codon 90 (GAC-->GCC).
Subject(s)
Alleles , HLA-A Antigens/genetics , Histocompatibility Testing , Amino Acid Sequence , Amino Acid Substitution/genetics , Base Sequence , Codon/genetics , Exons/genetics , Family Health , Fanconi Syndrome/diagnosis , Fanconi Syndrome/genetics , Female , Frameshift Mutation , Genetic Predisposition to Disease/genetics , HLA-A11 Antigen , Humans , Molecular Sequence Data , Polymerase Chain Reaction , Polymorphism, Single Nucleotide/genetics , Sequence Alignment , Sequence Analysis, DNAABSTRACT
We investigated the expression of different cell adhesion molecules on cord blood (CB) and bone marrow (BM) CD34+/CD38+ and CD34+/CD38- cells. CD11a and CD62L were more expressed in CB than in BM CD34+/CD38- subset, suggesting a possible advantage in homing and engraftment. A short exposure to various cytokines increased CD62L expression only in the more differentiated CB and BM CD34+/CD38+ cells.
Subject(s)
Antigens, CD , Bone Marrow Cells/cytology , Cell Adhesion Molecules/analysis , Fetal Blood/cytology , Hematopoietic Stem Cells/chemistry , ADP-ribosyl Cyclase , ADP-ribosyl Cyclase 1 , Antigens, CD34/analysis , Antigens, Differentiation/analysis , Bone Marrow Cells/chemistry , Fetal Blood/chemistry , Flow Cytometry , Humans , L-Selectin/analysis , Lymphocyte Function-Associated Antigen-1/analysis , Membrane Glycoproteins , NAD+ Nucleosidase/analysisABSTRACT
In eleven patients with dementia senilis circadian rhythms of hematocrit, hemoglobin, erythrocytes, leukocytes, serum iron and transferrin were studied by means of the cosinor method. An evident circadian rhythm was observed for all blood components, except leukocytes. There were no significant differences between patients with dementia senilis and healthy aged persons as far as hematocrit, hemoglobin, erythrocytes, and leukocytes are concerned. Acrophase for serum iron and transferrin occurs a little earlier and mesor is lower than in healthy aged persons.
