Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
J Virol Methods ; 163(2): 229-33, 2010 Feb.
Article in English | MEDLINE | ID: mdl-19819263

ABSTRACT

Previous and new PCRs for KHV detection were compared by estimation of their sensitivity in recognizing KHV DNA in plasmids, cell culture extracted KHV DNA and total DNA obtained from field tissue samples. A modified real-time PCR (Gilad et al., 2004), combined with an internal control system (IC2, Hoffmann et al., 2006) in a duplex assay, was used as a "gold standard". The lowest reliably determined virus concentration between, 5 and 10 KHV DNA genomic equivalents, was found by real-time PCR (Gilad et al., 2004), nested PCR (Bergmann et al., 2006) and one-tube semi-nested PCR. All other published and unpublished PCRs, as well as the commercial Loopamp, recognized KHV DNA at higher concentrations only. Additionally, KHV variants, newly adapted to European conditions, which could not be detected by PCR according to Bercovier et al. (2005) were found in two field samples from carp and koi from different regions of Germany. A negative influence of sample pooling was shown with field samples tested by real-time PCR.


Subject(s)
Carps/virology , DNA, Viral/isolation & purification , Fish Diseases/diagnosis , Herpesviridae Infections/veterinary , Herpesviridae/isolation & purification , Polymerase Chain Reaction/methods , Animals , DNA, Viral/genetics , Fish Diseases/virology , Germany , Herpesviridae/genetics , Herpesviridae Infections/diagnosis , Herpesviridae Infections/virology , Polymerase Chain Reaction/standards , Reference Standards , Sensitivity and Specificity
SELECTION OF CITATIONS
SEARCH DETAIL
...