Disruption of BMP4 signaling is associated with laryngeal birth defects in a mouse model.

Laryngeal birth defects are considered rare, but they can be life-threatening conditions. The BMP4 gene plays an important role in organ development and tissue remodeling throughout life. Here we examined its role in laryngeal development complementing similar efforts for the lung, pharynx, and cranial base. Our goal was to determine how different imaging techniques contribute to a better understanding of the embryonic anatomy of the normal and diseased larynx in small specimens. Contrast-enhanced micro CT images of embryonic larynx tissue from a mouse model with Bmp4 deletion informed by histology and whole-mount immunofluorescence were used to reconstruct the laryngeal cartilaginous framework in three dimensions. Laryngeal defects included laryngeal cleft, laryngeal asymmetry, ankylosis and atresia. Results implicate BMP4 in laryngeal development and show that the 3D reconstruction of laryngeal elements provides a powerful approach to visualize laryngeal defects and thereby overcoming shortcomings of 2D histological sectioning and whole mount immunofluorescence.

Radiographic analysis of vocal tract length and its relation to overall body size in two canid species.

Body size is an important determinant of resource and mate competition in many species. Competition is often mediated by conspicuous vocal displays, which may help to intimidate rivals and attract mates by providing honest cues to signaler size. Fitch proposed that vocal tract resonances (or formants) should provide particularly good, or honest, acoustic cues to signaler size because they are determined by the length of the vocal tract, which in turn, is hypothesized to scale reliably with overall body size. There is some empirical support for this hypothesis, but to date, many of the effects have been either mixed for males compared with females, weaker than expected in one or the other sex, or complicated by sampling issues. In this paper, we undertake a direct test of Fitch's hypothesis in two canid species using large samples that control for age- and sex-related variation. The samples involved radiographic images of 120 Portuguese water dogs Canis lupus familiaris and 121 Russian silver foxes Vulpes vulpes. Direct measurements were made of vocal tract length from X-ray images and compared against independent measures of body size. In adults of both species, and within both sexes, overall vocal tract length was strongly and significantly correlated with body size. Effects were strongest for the oral component of the vocal tract. By contrast, the length of the pharyngeal component was not as consistently related to body size. These outcomes are some of the clearest evidence to date in support of Fitch's hypothesis. At the same time, they highlight the potential for elements of both honest and deceptive body signaling to occur simultaneously via differential acoustic cues provided by the oral versus pharyngeal components of the vocal tract.

The harmonic-to-noise ratio applied to dog barks.

Dog barks are typically a mixture of regular components and irregular (noisy) components. The regular part of the signal is given by a series of harmonics and is most probably due to regular vibrations of the vocal folds, whereas noise refers to any nonharmonic (irregular) energy in the spectrum of the bark signal. The noise components might be due to chaotic vibrations of the vocal-fold tissue or due to turbulence of the air. The ratio of harmonic to nonharmonic energy in dog barks is quantified by applying the harmonics-to-noise ratio (HNR). Barks of a single dog breed were recorded in the same behavioral context. Two groups of dogs were considered: a group of ten healthy dogs (the normal sample), and a group of ten unhealthy dogs, i.e., dogs treated in a veterinary clinic (the clinic sample). Although the unhealthy dogs had no voice disease, differences in emotion or pain or impacts of surgery might have influenced their barks. The barks of the dogs were recorded for a period of 6 months. The HNR computation is based on the Fourier spectrum of a 50-ms section from the middle of the bark. A 10-point moving average curve of the spectrum on a logarithmic scale is considered as estimator of the noise level in the bark, and the maximum difference of the original spectrum and the moving average is defined as the HNR measure. It is shown that a reasonable ranking of the voices is achievable based on the measurement of the HNR. The HNR-based classification is found to be consistent with perceptual evaluation of the barks. In addition, a multiparametric approach confirms the classification based on the HNR. Hence, it may be concluded that the HNR might be useful as a novel parameter in bioacoustics for quantifying the noise within a signal.

Nonlinear phenomena in the natural howling of a dog-wolf mix.

It was reported to the first author that a female dog-wolf mix showed anomalously rough-sounding vocalization. Spectral analysis of recordings of the vocalization revealed frequency occurrences of subharmonics, biphonation (two independent pitches) and chaos. Since these nonlinear phenomena are currently widely discussed as integral to mammalian vocalization [Wilden et al., Bioacoustics 9, 171-196 (1988)] or as indicators of vocal pathologies [Herzel et al., J. Speech Hearing Res. 37, 1008-1019 (1994); Riede et al., Z. Sgtkde 62 Suppl: 198-203 (1997)], we sought to understand the production mechanism of the observed vocal instabilities. First the frequency of nonlinear phenomena in the calls was determined for the female and four additional individuals. It turned out that these phenomena appear, but much less frequently in the repertoire of the four other animals. The larynges of the female and two other individuals were dissected post mortem. There was no apparent asymmetry of the vocal folds but a slight asymmetry of the arytenoid cartilages. The most pronounced difference, however, was an upward extension of both vocal folds of the female. This feature is reminiscent of "vocal lips" (syn. "vocal membranes") in some primates and bats. Spectral analysis of the female's voice showed clear similarities with an intensively studied voice of a human who produces biphonation intentionally. Finally, the possible communicative relevance of nonlinear phenomena is discussed.

Vocal tract length and acoustics of vocalization in the domestic dog (Canis familiaris).

The physical nature of the vocal tract results in the production of formants during vocalisation. In some animals (including humans), receivers can derive information (such as body size) about sender characteristics on the basis of formant characteristics. Domestication and selective breeding have resulted in a high variability in head size and shape in the dog (Canis familiaris), suggesting that there might be large differences in the vocal tract length, which could cause formant behaviour to affect interbreed communication. Lateral radiographs were made of dogs from several breeds ranging in size from a Yorkshire terrier (2.5 kg) to a German shepherd (50 kg) and were used to measure vocal tract length. In addition, we recorded an acoustic signal (growling) from some dogs. Significant correlations were found between vocal tract length, body mass and formant dispersion, suggesting that formant dispersion can deliver information about the body size of the vocalizer. Because of the low correlation between vocal tract length and the first formant, we predict a non-uniform vocal tract shape.

[40 years of the microcensus]. / 40 Jahre Mikrozensus.

PIP: This essay discusses the history, goals, scope, functions, methodology, and future of the German microcensus, which was begun in the Federal Republic of Germany in 1957. Particular attention is given to the issue of whether making participation voluntary would have a negative effect on data quality.^ieng

[Analyses of voluntary responses in the microcensus: are survey results distorted by voluntary responses?]. / Analysen zur Freiwilligkeit der Auskunftserteilung im Mikrozensus: sind Stichprobenergebnisse bei Freiwilliger Auskunftserteilung verzerrt?

PIP: An attempt is made to determine whether microcensus results in Germany are less reliable when responses to questions are voluntary rather than required. The analysis focuses on two questions that have been voluntary since 1991: highest level of general schooling completed and most recent occupational training completed. Data are from the 1991, 1992, and 1993 microcensuses. Earlier data are included for comparative purposes.^ieng

[First results of the microcensus of April 1993]. / Erste Ergebnisse des Mikrozensus April 1993.

PIP: Results of the April 1993 microcensus of Germany are presented, together with some comparative data for 1991 and 1992. Information is included on population growth, labor force participation, income, and foreigners. Differences between former East and West Germany are examined.^ieng

[On voluntary responding to microcensus questions]. / Zur Freiwilligkeit in der Auskunftserteilung im Mikrozensus.

PIP: The authors examine how the quality of German microcensus data has been affected by a 1990 law that made it voluntary rather than mandatory to respond to many questions. The analysis focuses on the year 1991 and on questions related to education and occupational training. Differences in the number of responses are analyzed according to region, age group, household size, community size, labor force participation, occupation, and whether the questions were asked orally or in writing.^ieng