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Folia Microbiol (Praha) ; 55(5): 489-96, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20941585

ABSTRACT

Bacterial clone libraries of the gut microbiota of nurtured and starved Cylindroiulus fulviceps specimens displayed the predominance of the phyla Bacteroidetes (55 and 37 %, respectively) and Proteobacteria (40 and 35 %, respectively) and a high similarity to bacteria previously detected in the intestinal tract of termites and beetles, which are known to harbor symbiotic bacteria essential for digestive activity. Bacterial isolates were dominated by Proteobacteria (74 %), followed by members of the phyla Actinobacteria, Firmicutes and Bacteroidetes. PCR-DGGE fingerprints of the gut samples showed that intestinal bacteria were affected by starvation, although the change was not significant.


Subject(s)
Arthropods/microbiology , Bacteria/genetics , Bacteria/isolation & purification , Cloning, Molecular/methods , DNA Fingerprinting/methods , Gastrointestinal Tract/microbiology , Actinobacteria/classification , Actinobacteria/genetics , Actinobacteria/isolation & purification , Animals , Bacteria/classification , Bacteriological Techniques , Bacteroidetes/classification , Bacteroidetes/genetics , Bacteroidetes/isolation & purification , Culture Media , DNA, Bacterial/genetics , Ecosystem , Electrophoresis, Agar Gel/methods , Metagenome , Molecular Sequence Data , Phylogeny , Proteobacteria/classification , Proteobacteria/genetics , Proteobacteria/isolation & purification , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA
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