Frequency-modulated multifunction lidar for anemometry, range finding, and velocimetry-1. Theory and signal processing.

Original waveforms and optimized signal processing are proposed for frequency-modulated continuous-wave lidar for range finding, velocimetry, and laser anemometry. For range finding, the aim of this signal processing is to extend lidar range and reduce ambiguities. Moreover, the effect of moderate atmospheric turbulence on lidar efficiency is analyzed for infinite and finite targets, taking into account wind-induced bistatism. For laser anemometry, the aim is to measure air speed at the shortest distance farther than the rotor-induced turbulent volume around the helicopter and to avoid parasitic echoes coming from clouds or hard targets in the vicinity of a helicopter.

Frequency-modulated multifunction lidar for anemometry, range finding, and velocimetry-2. Experimental results.

Frequency-modulated continuous-wave lidar is evaluated for range finding, velocimetry, and laser anemometry. An original signal processing and waveform calibration for range finding leads to a reduction of computational effort while preserving capability for long-range measurement. Multiple target distance measurement is also demonstrated. For laser anemometry, the aim is to avoid parasitic echoes in the vicinity of a helicopter and to measure the air speed at the shortest distance farther than the rotor-induced turbulent volume around the helicopter. Flight tests of this functionality and vortex ring state warning are demonstrated.

Sensitivity to acetic acid, ability to colonize abiotic surfaces and virulence potential of Listeria monocytogenes EGD-e after incubation on parsley leaves.

AIM: To investigate how the survival of Listeria monocytogenes on parsley leaves may affect its ability to sustain process-related harsh conditions and its virulence. METHODS AND RESULTS: Parsley seedlings were spot inoculated with stationary phase cells of L. monocytogenes EGD-e and incubated for 15 days. Each day, bacterial cells were harvested and enumerated, and their ability to survive acetic acid challenge (90 min, pH 4.0), to colonize abiotic surfaces and to grow as biofilms was assessed. After a 3-log decrease over the first 48 h, the population stabilized to about 10(6) CFU g(-1) until the sixth day. After the sixth day, L. monocytogenes was no longer detected, even after specific enrichment. Incubation on parsley leaves affected the ability of L. monocytogenes to survive acetic acid challenge (90 min, pH 4.0) and to adhere to stainless steel although the ability to grow as biofilm was preserved. To further investigate these physiological alterations, the mRNA levels of six target genes (bsh, clpC, groEL, inlA, opuC, prfA) was quantified using reverse transcription qPCR after 5 h of incubation on parsley leaves. A decrease was observed in all but one (bsh) target, including groEL and clpC which are involved in resistance to salt and acid. Moreover, the decrease in the levels of inlA, prfA and opuC transcripts after incubation on parsley suggested a repression of some genes involved in pathogenicity. In vitro assessment of mammalian cell adherence and invasion using Caco-2 cells confirmed the repression of the virulence factor InlA; however, the virulence potential in vivo in the chick embryo model was not affected. CONCLUSION: Listeria monocytogenes did undergo rapid changes to adapt its physiology to the phyllosphere. SIGNIFICANCE AND IMPACT OF THE STUDY: This study highlights the physiological changes undergone by L. monocytogenes during/after survival on parsley leaves.

Slow viral dynamics of hepatitis C virus genotype 4.

Patients infected with hepatitis C virus genotype 4 (HCV-4) respond to interferon alpha (IFN) as poorly as those infected with genotype 1. However, there is no information on the viral dynamics of HCV-4. Interferon-ribavirin treatment was administered to untreated patients infected with HCV-4. Viral load was assessed with Versant 3.0. Viral dynamics parameters were estimated based on the bi-phasic model for HCV during IFN treatment. Viral kinetics of HCV-4 follow a bi-phasic decline pattern also. The mean effectiveness of IFN in blocking production of HCV-4 was a decline of 77.8% during the first day of treatment. The half-life of free virions was estimated at 3.5 h and that of infected cells from 1.9 to over 70 days. The viral dynamics parameters of HCV-4 appear similar to those of HCV-1 and slower than those of HCV-2. HCV-4 infected patients should be grouped with those with HCV-1 when therapeutic schemes are considered in relation to genotype.