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1.
Analysis and preparation of chromosomal high-mobility group proteins by ion-exchange high-performance liquid chromatography.
Riffe, A; Delpech, M; Levy-Favatier, F; Boissel, J P; Kruh, J.
J Chromatogr ; 344: 332-8, 1985 Nov 08.
Article in English | MEDLINE | ID: mdl-4086556

Subject(s)
High Mobility Group Proteins/analysis , Amino Acids/analysis , Animals , Chromatography, High Pressure Liquid , Chromatography, Ion Exchange , Electrophoresis, Polyacrylamide Gel , High Mobility Group Proteins/isolation & purification , Rats , Rats, Inbred Strains
2.
A 25 000 dalton inhibitor of cAMP independent protein kinases present in rat liver HMG protein preparations.
Levy-Favatier, F; Delpech, M; Riffe, A; Kruh, J.
Biochem Biophys Res Commun ; 130(1): 149-56, 1985 Jul 16.
Article in English | MEDLINE | ID: mdl-2992459

ABSTRACT

A protein kinase inhibitor was found in rat liver cells as a component of HMG proteins. It is located in cytosol as well as in nuclei. It inhibits all tested cAMP independent protein kinases and has no effect on cAMP dependent protein kinases. This inhibitor is a 25 000 Da protein. It has no ATPase, phosphoprotein phosphatase or proteinase activity and is heat unstable.


Subject(s)
High Mobility Group Proteins/metabolism , Liver/enzymology , Protein Kinase Inhibitors , Adenosine Triphosphatases/metabolism , Animals , Cell Nucleus/enzymology , Cytosol/enzymology , Male , Molecular Weight , Peptide Hydrolases/metabolism , Phosphoprotein Phosphatases/metabolism , Rats , Rats, Inbred Strains
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