ABSTRACT
The potential of chromosomal damage associated with the effects of chlorine in drinking water was evaluated using chromosome aberrations and micronuclei as cytogenetic endpoints in the lymphocytes of peripheral blood of ewe lambs. The study assessed the in vivo effects of high chlorine doses (1.8 mg l(-1), based on Savo-Super disinfectant) in drinking water on the peripheral lymphocytes of sheep after 30 days. The frequency of aberrant cells (AB.C.) in the experimental and control groups was 31.80+/-13.68% AB.C. and 4.50+/-2.07% AB.C. respectively, and the increased AB.C. in the treated group was highly significant (P=/<0.001). In the experimental group chromatid breaks (26.20+/-10.47%) and gaps (24.20+/-13.94%) were the dominant types of aberrations, but statistically significant chromosome gaps and exchanges were also present. The frequency of micronuclei in peripheral lymphocytes of sheep in the control group was 21.17+/-4.36 per 1,000 binucleated cells and 64.20+/-22.51 per 1,000 binucleated cells in the experimental group. A significant increase in the frequency of micronuclei in peripheral lymphocytes of sheep was observed between the two groups (P=0.001).
Subject(s)
Chlorine/toxicity , Chromosome Aberrations/chemically induced , DNA Damage , Water Supply , Animals , Female , Lymphocytes , SheepABSTRACT
Two strains of lactobacilli (Lactobacillus acidophilus T-135 and Lactobacillus plantarum 4/97) were selected in order to study their inhibitory properties against frequent udder pathogens (Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis, Salmonella enteritidis and Bacillus pumilus), their production of organic acids as well as their ability to survive on the teat skin, the teat duct mucosa and in a lipoid emulsion. Both strains inhibited the tested pathogenic microbes and survived on the investigated surfaces and in an emulsion for more than 6 hours and 11 days, respectively.
Subject(s)
Antibiosis , Lactobacillus acidophilus/physiology , Lactobacillus/physiology , Mammary Glands, Animal/microbiology , Probiotics , Animals , Bacillus/growth & development , Cattle , Dairying/methods , Escherichia coli/growth & development , Female , Salmonella enteritidis/growth & development , Staphylococcus aureus/growth & development , Streptococcus/growth & developmentABSTRACT
A cytogenetic analysis of peripheral blood lymphocytes was carried out on breeding boars (n = 5) on a farm where viral infections (porcine parvovirosis) had occurred. Several different types of chromosomal aberrations were observed including chromatid breaks (23.60 +/- 2.88%), exchanges (9.60 +/- 8.99%), and fragments (4.26 +/- 3.31%). In addition, 2.94 +/- 1.74% of cells in metaphase showed pulverization. Cells with multiple aberrations were seen in two of five boars. The chromosomal damage in the boars may have been induced by a genotoxic agent such as a viral infection, and may also be related to the reproductive impairment of the pigs.
