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1.
Front Plant Sci ; 7: 1771, 2016.
Article in English | MEDLINE | ID: mdl-27965684

ABSTRACT

Seven blackleg resistance (R) genes (Rlm1, Rlm2, Rlm3, Rlm4, LepR1, LepR2 & LepR3) were each introgressed into a common susceptible B. napus doubled-haploid (DH) line through reciprocal back-crossing, producing single-R gene introgression lines (ILs) for use in the pathological and molecular study of Brassica-Leptosphaeria interactions. The genomic positions of the R genes were defined through molecular mapping and analysis with transgenic L. maculans isolates was used to confirm the identity of the introgressed genes where possible. Using L. maculans isolates of contrasting avirulence gene (Avr) profiles, we preformed extensive differential pathology for phenotypic comparison of the ILs to other B. napus varieties, demonstrating the ILs can provide for the accurate assessment of Avr-R gene interactions by avoiding non-Avr dependant alterations to resistance responses which can occur in some commonly used B. napus varieties. Whole-genome SNP-based assessment allowed us to define the donor parent introgressions in each IL and provide a strong basis for comparative molecular dissection of the pathosystem.

2.
BMC Plant Biol ; 16(1): 183, 2016 08 24.
Article in English | MEDLINE | ID: mdl-27553246

ABSTRACT

BACKGROUND: Resistance to the blackleg disease of Brassica napus (canola/oilseed rape), caused by the hemibiotrophic fungal pathogen Leptosphaeria maculans, is determined by both race-specific resistance (R) genes and quantitative resistance loci (QTL), or adult-plant resistance (APR). While the introgression of R genes into breeding material is relatively simple, QTL are often detected sporadically, making them harder to capture in breeding programs. For the effective deployment of APR in crop varieties, resistance QTL need to have a reliable influence on phenotype in multiple environments and be well defined genetically to enable marker-assisted selection (MAS). RESULTS: Doubled-haploid populations produced from the susceptible B. napus variety Topas and APR varieties AG-Castle and AV-Sapphire were analysed for resistance to blackleg in two locations over 3 and 4 years, respectively. Three stable QTL were detected in each population, with two loci appearing to be common to both APR varieties. Physical delineation of three QTL regions was sufficient to identify candidate defense-related genes, including a cluster of cysteine-rich receptor-like kinases contained within a 49 gene QTL interval on chromosome A01. Individual L. maculans isolates were used to define the physical intervals for the race-specific R genes Rlm3 and Rlm4 and to identify QTL common to both field studies and the cotyledon resistance response. CONCLUSION: Through multi-environment QTL analysis we have identified and delineated four significant and stable QTL suitable for MAS of quantitative blackleg resistance in B. napus, and identified candidate genes which potentially play a role in quantitative defense responses to L. maculans.


Subject(s)
Ascomycota/physiology , Brassica napus/genetics , Plant Diseases/genetics , Protein Kinases/genetics , Quantitative Trait Loci , Brassica napus/immunology , Brassica napus/microbiology , Chromosome Mapping , Chromosomes, Plant/genetics , Phenotype , Plant Diseases/immunology , Plant Diseases/microbiology , Protein Kinases/metabolism
3.
Can J Microbiol ; 62(10): 836-850, 2016 Oct.
Article in English | MEDLINE | ID: mdl-27503454

ABSTRACT

Mitogen-activated protein kinases (MAPKs) play a central role in transferring signals and regulating gene expression in response to extracellular stimuli. An ortholog of the Saccharomyces cerevisiae cell wall integrity MAPK was identified in the phytopathogenic fungus Sclerotinia sclerotiorum. Disruption of the S. sclerotiorum Smk3 gene severely reduced virulence on intact host plant leaves but not on leaves stripped of cuticle wax. This was attributed to alterations in hyphal apical dominance leading to the inability to aggregate and form infection cushions. The mutation also caused loss of the ability to produce sclerotia, increased aerial hyphae formation, and altered hyphal hydrophobicity and cell wall integrity. Mutants had slower radial expansion rates on solid media but more tolerance to elevated temperatures. Loss of the SMK3 cell wall integrity MAPK appears to have impaired the ability of S. sclerotiorum to sense its surrounding environment, leading to misregulation of a variety of functions. Many of the phenotypes were similar to those observed in S. sclerotiorum adenylate cyclase and SMK1 MAPK mutants, suggesting that these signaling pathways co-regulate aspects of fungal growth, physiology, and pathogenicity.


Subject(s)
Ascomycota/enzymology , Fungal Proteins/physiology , Mitogen-Activated Protein Kinases/physiology , Plant Diseases/microbiology , Adenylyl Cyclases/genetics , Ascomycota/growth & development , Ascomycota/pathogenicity , Brassica napus/microbiology , Cell Wall/metabolism , Conserved Sequence , Gene Expression , Hyphae/enzymology , Hyphae/growth & development , Hyphae/pathogenicity , Membrane Proteins , Mycelium/enzymology , Mycelium/growth & development , Mycelium/pathogenicity , Phenotype , Phosphorylation , Protein Processing, Post-Translational , Saccharomyces cerevisiae Proteins , Sequence Homology, Amino Acid , Signal Transduction , Virulence/genetics
4.
BMC Plant Biol ; 14: 387, 2014 Dec 31.
Article in English | MEDLINE | ID: mdl-25551287

ABSTRACT

BACKGROUND: The protection of canola (Brassica napus) crops against blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, is largely mediated by race-specific resistance genes (R-genes). While many R-genes effective against blackleg disease have been identified in Brassica species, information of the precise genomic locations of the genes is limited. RESULTS: In this study, the Rlm2 gene for resistance to blackleg, located on chromosome A10 of the B. napus cultivar 'Glacier', was targeted for fine mapping. Molecular markers tightly linked to the gene were developed for use in mapping the resistance locus and defining the physical interval in B. napus. Rlm2 was localised to a 5.8 cM interval corresponding to approximately 873 kb of the B. napus chromosome A10. CONCLUSION: The recently-cloned B. napus R-gene, LepR3, occupies the same region of A10 as Rlm2 and analysis of the putative B. napus and B. rapa genes in the homologous region identified several additional candidate defense-related genes that may control Rlm2 function.


Subject(s)
Brassica napus/genetics , Brassica napus/microbiology , Chromosomes, Plant/genetics , Disease Resistance/genetics , Plant Proteins/genetics , Ascomycota/physiology , Brassica napus/metabolism , Breeding , Chromosome Mapping , Crops, Agricultural/genetics , Crops, Agricultural/metabolism , Crops, Agricultural/microbiology , Genetic Markers , Plant Diseases/genetics , Plant Diseases/microbiology , Plant Proteins/metabolism
5.
Can J Microbiol ; 59(2): 79-86, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23461514

ABSTRACT

Sclerotinia sclerotiorum releases a battery of polygalacturonases (PGs) during infection, which the host plant may cope with through production of polygalacturonase inhibitor proteins (PGIPs). To study the interaction between S. sclerotiorum PGs and Brassica napus PGIPs, 5 S. sclerotiorum PGs and 4 B. napus PGIPs were expressed in Pichia pastoris. SsPG3, SsPG6, and BnPGIP1 were successfully produced in the yeast system, and BnPGIP1 inhibited SsPG6 enzymatic activity in vitro. SsPG3 and SsPG6 both induced light-dependent necrosis when infiltrated into leaves, which was reduced in an Arabidopsis thaliana line expressing BnPGIP2 and to a lesser extent in a line expressing BnPGIP1. The line expressing BnPGIP2 also exhibited a delay in the onset of symptoms upon S. sclerotiorum inoculation, but no long-term effect on S. sclerotiorum disease progression was observed. The P. pastoris system was found to be suitable for expressing high levels of some S. sclerotiorum PGs, but PGIP interaction studies were best performed in planta. Arabidopsis thaliana forms necrotic lesions upon infiltration of PGs, is susceptible to S. sclerotiorum, and is easily transformed, and thus, is well-suited for the qualitative study of PG-PGIP interactions.


Subject(s)
Ascomycota/enzymology , Brassica napus/genetics , Brassica napus/microbiology , Enzyme Inhibitors/metabolism , Plant Proteins/genetics , Arabidopsis/genetics , Arabidopsis/microbiology , Brassica napus/metabolism , Gene Expression , Plant Proteins/metabolism , Plants, Genetically Modified/genetics , Plants, Genetically Modified/metabolism , Polygalacturonase/antagonists & inhibitors , Polygalacturonase/metabolism
6.
Theor Appl Genet ; 126(2): 307-15, 2013 Feb.
Article in English | MEDLINE | ID: mdl-22733446

ABSTRACT

Blackleg, caused by Leptosphaeria maculans, is one of the most economically important diseases of Brassica napus worldwide. Two blackleg-resistant lines, 16S and 61446, were developed through interspecific hybridization between B. napus and B. rapa subsp. sylvestris and backcrossing to B. napus. Classical genetic analysis demonstrated that a single recessive gene in both lines conferred resistance to L. maculans and that the resistance alleles were allelic. Using BC(1) progeny derived from each resistant plant, this locus was mapped to B. napus linkage group N6 and was flanked by microsatellite markers sN2189b and sORH72a in an interval of about 10 cM, in a region equivalent to about 6 Mb of B. rapa DNA sequence. This new resistance gene locus was designated as LepR4. The two lines were evaluated for resistance to a wide range of L. maculans isolates using cotyledon inoculation tests under controlled environment conditions, and for stem canker resistance in blackleg field nurseries. Results indicated that line 16S, carrying LepR4a, was highly resistant to all isolates tested on cotyledons and had a high level of stem canker resistance under field conditions. Line 61446, carrying LepR4b, was only resistant to some of the isolates tested on cotyledons and was weakly resistant to stem canker under field conditions.


Subject(s)
Ascomycota/physiology , Brassica napus/genetics , Brassica napus/microbiology , Disease Resistance/genetics , Genes, Plant/genetics , Plant Diseases/microbiology , Quantitative Trait Loci , Ascomycota/pathogenicity , Brassica napus/immunology , Chromosome Mapping , Chromosomes, Plant/genetics , Cotyledon/genetics , Cotyledon/immunology , Cotyledon/microbiology , Crosses, Genetic , Gene Expression Regulation, Plant , Immunity, Innate , Microsatellite Repeats , Plant Diseases/genetics , Plant Diseases/immunology
7.
Can J Microbiol ; 58(5): 605-16, 2012 May.
Article in English | MEDLINE | ID: mdl-22524557

ABSTRACT

Sclerotinia sclerotiorum releases hydrolytic enzymes that sequentially degrade the plant cuticle, middle lamellae, and primary and secondary cell walls. The cuticle was found to be a barrier to S. sclerotiorum infection, as leaves stripped of epicuticular wax were more rapidly colonized. Consequently, the factors affecting the regulation of genes encoding polygalacturonase 1 (SsPG1) and a newly identified cutinase (SsCUTA) were examined. In vitro, SsCutA transcripts were detected within 1 h postinoculation of leaves, and expression was primarily governed by contact of mycelia with solid surfaces. Expression of SsPg1 was moderately induced by contact with solid surfaces including the leaf, and expression was restricted to the expanding margin of the lesion as the infection progressed. SsPg1 expression was induced by carbohydrate starvation but repressed by galacturonic acid. Glucose supported a basal level of SsPg1 expression but accentuated expression when provided to mycelia used to inoculate leaves. These observations were contrary to earlier reports indicating that glucose repressed SsPg1 expression while galacturonic acid induced expression. Pharmacological studies showed that disruption of calcium signalling affected SsCutA and SsPg1 expression and decreased S. sclerotiorum virulence, whereas elevated cAMP levels reduced virulence without affecting gene expression. The mechanisms involved in coordinating the expression of S. sclerotiorum hydrolytic enzymes throughout the various stages of the infection are discussed.


Subject(s)
Ascomycota/enzymology , Carboxylic Ester Hydrolases/metabolism , Plant Diseases/microbiology , Polygalacturonase/metabolism , Amino Acid Sequence , Ascomycota/genetics , Ascomycota/pathogenicity , Calcium Signaling , Carboxylic Ester Hydrolases/genetics , Cyclic AMP/metabolism , Fungal Proteins/genetics , Fungal Proteins/metabolism , Gene Expression , Gene Expression Regulation, Fungal , Glucose/pharmacology , Hexuronic Acids/pharmacology , Molecular Sequence Data , Plant Leaves/microbiology , Polygalacturonase/genetics
8.
Theor Appl Genet ; 124(3): 505-13, 2012 Feb.
Article in English | MEDLINE | ID: mdl-22038486

ABSTRACT

AvrLepR1 of the fungal pathogen Leptosphaeria maculans is the avirulence gene that corresponds to Brassica LepR1, a plant gene controlling dominant, race-specific resistance to this pathogen. An in vitro cross between the virulent L. maculans isolate, 87-41, and the avirulent isolate, 99-56, was performed in order to map the AvrLepR1 gene. The disease reactions of the 94 of the resulting F(1) progenies were tested on the canola line ddm-12-6s-1, which carries LepR1. There were 44 avirulent progenies and 50 virulent progenies suggesting a 1:1 segregation ratio and that the avirulence of 99-56 on ddm-12-6s-1 is controlled by a single gene. Tetrad analysis also indicated a 1:1 segregation ratio. The AvrLepR1 gene was positioned on a genetic map of L. maculans relative to 259 sequence-related amplified polymorphism (SRAP) markers, two cloned avirulence genes (AvrLm1 and AvrLm4-7) and the mating type locus (MAT1). The genetic map consisted of 36 linkage groups, ranging in size from 13.1 to 163.7 cM, and spanned a total of 2,076.4 cM. The AvrLepR1 locus was mapped to linkage group 4, in the 13.1 cM interval flanked by the SRAP markers SBG49-110 and FT161-223. The AvrLm4-7 locus was also positioned on linkage group 4, close to but distinct from the AvrLepR1 locus, in the 5.4 cM interval flanked by FT161-223 and P1314-300. This work will make possible the further characterization and map-based cloning of AvrLepR1. A combination of genetic mapping and pathogenicity tests demonstrated that AvrLepR1 is different from each of the L. maculans avirulence genes that have been characterized previously.


Subject(s)
Ascomycota/genetics , Brassica napus/genetics , Disease Resistance/genetics , Genes, Fungal/genetics , Genes, Plant/genetics , Plant Diseases/microbiology , Ascomycota/pathogenicity , Brassica napus/microbiology , Chromosome Mapping , Crosses, Genetic , DNA Primers/genetics , Genetic Markers/genetics , Virulence/genetics
9.
Mol Plant Pathol ; 11(2): 283-91, 2010 Mar.
Article in English | MEDLINE | ID: mdl-20447277

ABSTRACT

White blister rust caused by Albugo candida (Pers.) Kuntze is a common and often devastating disease of oilseed and vegetable brassica crops worldwide. Physiological races of the parasite have been described, including races 2, 7 and 9 from Brassica juncea, B. rapa and B. oleracea, respectively, and race 4 from Capsella bursa-pastoris (the type host). A gene named WRR4 has been characterized recently from polygenic resistance in the wild brassica relative Arabidopsis thaliana (accession Columbia) that confers broad-spectrum white rust resistance (WRR) to all four of the above Al. candida races. This gene encodes a TIR-NB-LRR (Toll-like/interleukin-1 receptor-nucleotide binding-leucine-rich repeat) protein which, as with other known functional members in this subclass of intracellular receptor-like proteins, requires the expression of the lipase-like defence regulator, enhanced disease susceptibility 1 (EDS1). Thus, we used RNA interference-mediated suppression of EDS1 in a white rust-resistant breeding line of B. napus (transformed with a construct designed from the A. thaliana EDS1 gene) to determine whether defence signalling via EDS1 is functionally intact in this oilseed brassica. The eds1-suppressed lines were fully susceptible following inoculation with either race 2 or 7 isolates of Al. candida. We then transformed white rust-susceptible cultivars of B. juncea (susceptible to race 2) and B. napus (susceptible to race 7) with the WRR4 gene from A. thaliana. The WRR4-transformed lines were resistant to the corresponding Al. candida race for each host species. The combined data indicate that WRR4 could potentially provide a novel source of white rust resistance in oilseed and vegetable brassica crops.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/genetics , Brassica/genetics , Crops, Agricultural/genetics , Immunity, Innate/genetics , Plant Diseases/immunology , Proteins/genetics , Arabidopsis Proteins/chemistry , Brassica/microbiology , Chromosome Segregation/genetics , Genes, Plant/genetics , Immunity, Innate/immunology , Leucine-Rich Repeat Proteins , Oomycetes/pathogenicity , Plant Diseases/genetics , Plant Diseases/microbiology , Plant Oils/metabolism , Plants, Genetically Modified , Protein Structure, Tertiary , RNA Interference , Seeds/genetics , Seeds/microbiology , Virulence
10.
Mol Plant Pathol ; 11(1): 43-53, 2010 Jan.
Article in English | MEDLINE | ID: mdl-20078775

ABSTRACT

Successful host colonization by necrotrophic plant pathogens requires the induction of plant cell death to provide the nutrients needed for infection establishment and progression. We have cloned two genes encoding necrosis and ethylene-inducing peptides from Sclerotinia sclerotiorum, which we named SsNep1 and SsNep2. The peptides encoded by these genes induce necrosis when expressed transiently in tobacco leaves. SsNep1 is expressed at a very low level relative to SsNep2 during infection. The expression of SsNep2 was induced by contact with solid surfaces and occurred in both the necrotic zone and at the leading margin of the infection. SsNep2 expression was dependent on calcium and cyclic adenosine monophosphate signalling, as compounds affecting these pathways reduced or abolished SsNep2 expression coincident with a partial or total loss of virulence.


Subject(s)
Ascomycota/pathogenicity , Ethylenes/metabolism , Peptide Biosynthesis , Amino Acid Sequence , Calcium Signaling , Cyclic AMP/metabolism , Molecular Sequence Data , Necrosis , Peptides/chemistry , Sequence Homology, Amino Acid
11.
Plant Physiol Biochem ; 47(7): 653-6, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19345111

ABSTRACT

Gene redundancy due to polyploidization provides a selective advantage for plant adaptation. We examined the expression patterns of two peroxidase genes (BnPOX1 and BnPOX2) in the natural allotetraploid Brassica napus and the model diploid progenitors Brassica rapa (Br) and Brassica oleracea (Bo) in response to the fungal pathogen Sclerotinia sclerotiorum. We demonstrated that the Bo homeolog of BnPOX1 was up-regulated after infection, while both BnPOX2 homeologs were down-regulated. A bias toward reciprocal expression of the homeologs of BnPOX1 in different organs in the natural allotetraploid of B. napus was also observed. These results suggest that subfunctionalization of the duplicated BnPOX genes after B. napus polyploidization as well as subneofunctionalization of the homeologs in response to this specific biotic stress has occurred. Retention of expression patterns in the diploid progenitors and the natural allotetraploid in some organs indicates that the function of peroxidase genes has been conserved during evolution.


Subject(s)
Brassica napus/genetics , Gene Expression Regulation, Plant , Gene Expression , Genes, Duplicate , Genes, Plant , Peroxidase/genetics , Plant Diseases/microbiology , Ascomycota , Brassica napus/metabolism , Multigene Family , Peroxidase/metabolism , Plant Diseases/genetics , Plant Structures , Polyploidy
12.
Mol Plant Microbe Interact ; 21(6): 757-68, 2008 Jun.
Article in English | MEDLINE | ID: mdl-18624640

ABSTRACT

White blister rust in the Brassicaceae is emerging as a superb model for exploring how plant biodiversity has channeled speciation of biotrophic parasites. The causal agents of white rust across a wide breadth of cruciferous hosts currently are named as variants of a single oomycete species, Albugo candida. The most notable examples include a major group of physiological races that each are economically destructive in a different vegetable or oilseed crop of Brassica juncea (A. candida race 2), B. rapa (race 7), or B. oleracea (race 9); or parasitic on wild crucifers such as Capsella bursa-pastoris (race 4). Arabidopsis thaliana is innately immune to these races of A. candida under natural conditions; however, it commonly hosts its own molecularly distinct subspecies of A. candida (A. candida subsp. arabidopsis). In the laboratory, we have identified several accessions of Arabidopsis thaliana (e.g.,. Ws-3) that can permit varying degrees of rust development following inoculation with A. candida races 2, 4, and 7, whereas race 9 is universally incompatible in Arabidopsis thaliana and nonrusting resistance is the most prevalent outcome of interactions with the other races. Subtle variation in resistance phenotypes is evident, observed initially with an isolate of A. candida race 4, indicating additional genetic variation. Therefore, we used the race 4 isolate for map-based cloning of the first of many expected white rust resistance (WRR) genes. This gene was designated WRR4 and encodes a cytoplasmic toll-interleukin receptor-like nucleotide-binding leucine-rich repeat receptor-like protein that confers a dominant, broad-spectrum white rust resistance in the Arabidopsis thaliana accession Columbia to representative isolates of A. candida races 2, 4, 7, and 9, as verified by transgenic expression of the Columbia allele in Ws-3. The WRR4 protein requires functional expression of the lipase-like protein EDS1 but not the paralogous protein PAD4, and confers full immunity that masks an underlying nonhypersensitive incompatibility in Columbia to A. candida race 4. This residual incompatibility is independent of functional EDS1.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/genetics , Oomycetes/growth & development , Plant Diseases/genetics , Arabidopsis/microbiology , Arabidopsis Proteins/metabolism , Arabidopsis Proteins/physiology , Cloning, Molecular , Gene Expression Regulation, Plant , Immunity, Innate/genetics , Immunity, Innate/immunology , Models, Genetic , Mutation , Oomycetes/classification , Oomycetes/isolation & purification , Plant Diseases/microbiology , Plants, Genetically Modified , RNA, Messenger/genetics , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Species Specificity
13.
Planta ; 228(2): 241-53, 2008 Jul.
Article in English | MEDLINE | ID: mdl-18431596

ABSTRACT

Most plants encode a limited set of polygalacturonase inhibitor (PGIP) genes that may be involved in aspects of plant development, but more importantly in the inactivation of polygalacturonases (PG) secreted by pathogens. Previously, we characterized two Brassica napus PGIP genes, BnPgip1 and BnPgip2, which were differentially expressed in response to pathogen infection and wounding. Here we report that the B. napus genome encodes a set of at least 16 PGIP genes that are similar to BnPgip1 or BnPgip2. This is the largest Pgip gene family reported to date. Comparison of the BnPGIPs revealed several sites within the xxLxLxx region of leucine rich repeats that form beta-sheets along the interacting face of the PGIP that are hypervariable and represent good candidates for generating PGIP diversity. Characterization of the regulatory regions and RT-PCR studies with gene-specific primers revealed that individual genes were differentially responsive to pathogen infection, mechanical wounding and signaling molecules. Many of the BnPgip genes responded to infection by the necrotic pathogen, Sclerotinia sclerotiorum; however, these genes were also induced either by jasmonic acid, wounding and salicylic acid or some combination thereof. The large number of PGIPs and the differential manner in which they are regulated likely ensures that B. napus can respond to attack from a broad spectrum of pathogens and pests.


Subject(s)
Ascomycota/physiology , Brassica napus/physiology , Host-Pathogen Interactions , Plant Diseases/immunology , Plant Proteins/genetics , Amino Acid Sequence , Brassica napus/microbiology , Cyclopentanes/metabolism , Gene Expression Regulation, Plant , Molecular Sequence Data , Multigene Family , Oxylipins/metabolism , Phylogeny , Plant Proteins/metabolism , Salicylic Acid/metabolism
14.
Genome ; 51(1): 64-72, 2008 Jan.
Article in English | MEDLINE | ID: mdl-18356940

ABSTRACT

The spectrum of resistance to isolates of Leptosphaeria maculans and the map location of a new blackleg resistance gene found in the canola cultivar Brassica napus 'Surpass 400' are described. Two blackleg resistance genes, LepR1 and LepR2, from B. rapa subsp. sylvestris and introgressed in B. napus were identified previously. 'Surpass 400' also has blackleg resistance introgressed from B. rapa subsp. sylvestris. Using 31 diverse isolates of L. maculans, the disease reaction of 'Surpass 400' was compared with those of the resistant breeding lines AD9 (which contains LepR1), AD49 (which contains LepR2), and MC1-8 (which contains both LepR1 and LepR2). The disease reaction on 'Surpass 400' was different from those observed on AD9 and MC1-8, indicating that 'Surpass 400' carries neither LepR1 nor both LepR1 and LepR2 in combination. Disease reactions of 'Surpass 400' to most of the isolates tested were indistinguishable from those of AD49, which suggested 'Surpass 400' might contain LepR2 or a similar resistance gene. Classical genetic analysis of F1 and BC1 plants showed that a dominant allele conferred resistance to isolates of L. maculans in 'Surpass 400'. The resistance gene, which mapped to B. napus linkage group N10 in an interval of 2.9 cM flanked by microsatellite markers sR12281a and sN2428Rb and 11.7 cM below LepR2, was designated LepR3. A 9 cM region of the B. napus genome containing LepR3 was found to be syntenic with a segment of Arabidopsis chromosome 5.


Subject(s)
Brassica napus/genetics , Genes, Plant , Arabidopsis/genetics , Ascomycota/isolation & purification , Chromosome Mapping , Crosses, Genetic , Genomics , Immunity, Innate , Microsatellite Repeats , Plant Diseases/microbiology , Synteny
15.
Phytochemistry ; 69(4): 894-910, 2008 Feb.
Article in English | MEDLINE | ID: mdl-18039546

ABSTRACT

The metabolites produced in leaves of the oilseeds canola and rapeseed (Brassica rapa L.) inoculated with either different races of the biotroph Albugo candida or sprayed with CuCl(2) were determined. This investigation established consistent phytoalexin (spirobrassinin, cyclobrassinin, and rutalexin) and phytoanticipin (indolyl-3-acetonitrile, arvelexin, caulilexin C, and 4-methoxyglucobrassicin) production in canola and rapeseed in response to both biotic and abiotic elicitation. In addition, a wide number of polar metabolites were isolated from infected leaves, including six new phenylpropanoids and two new flavonoids. The extractable chemical components of zoosporangia of A. candida and the anti-oomycete activity of phytoalexins were determined as well. Overall, the results suggest that during the initial stage of the interaction, leaves of B. rapa have a similar response to virulent and avirulent races of A. candida, with respect to the accumulation of chemical defenses. After this stage, despite the higher phytoalexin concentration, the "compatible" races could overcome the plant defense system for further infection, but growth of the "incompatible" races was inhibited. Since results of bioassays showed that cyclobrassinin and brassilexin were more inhibitory to A. candida than rutalexin, the apparent redirection of the phytoalexin pathway towards rutalexin, avoiding cyclobrassinin and brassilexin accumulation might be caused by the pathogen. Alternatively, A. candida might be able to detoxify both cyclobrassinin and brassilexin, similar to necrotrophic plant pathogens. Overall, the correlation between phytoalexin production in infected or stressed leaves and the outcome of the plant-pathogen interaction suggested that A. candida was able to elude the plant defense mechanisms by, for example, redirecting the phytoalexin biosynthetic pathway.


Subject(s)
Brassica napus/metabolism , Brassica rapa/metabolism , Oomycetes/growth & development , Terpenes/metabolism , Brassica napus/drug effects , Brassica napus/microbiology , Brassica rapa/drug effects , Brassica rapa/microbiology , Chromatography, High Pressure Liquid , Copper/pharmacology , Gene Expression Regulation, Plant/drug effects , Magnetic Resonance Spectroscopy , Molecular Structure , Plant Leaves/drug effects , Plant Leaves/metabolism , Plant Leaves/microbiology , Sesquiterpenes , Spectrometry, Mass, Electrospray Ionization , Phytoalexins
16.
FEMS Microbiol Lett ; 251(2): 177-84, 2005 Oct 15.
Article in English | MEDLINE | ID: mdl-16112822

ABSTRACT

Sclerotinia sclerotiorum is unusual among necrotrophic pathogens in its requirement for senescent tissues to establish an infection and to complete the life cycle. A model for the infection process has emerged whereby the pathogenic phase is bounded by saprophytic phases; the distinction being that the dead tissues in the latter are generated by the actions of the pathogen. Initial colonization of dead tissue provides nutrients for pathogen establishment and resources to infect healthy plant tissue. The early pathogenicity stage involves production of oxalic acid and the expression of cell wall degrading enzymes, such as specific isoforms of polygalacturonase (SSPG1) and protease (ASPS), at the expanding edge of the lesion. Such activities release small molecules (oligo-galacturonides and peptides) that serve to induce the expression of a second wave of degradative enzymes that collectively bring about the total dissolution of the plant tissue. Oxalic acid and other metabolites and enzymes suppress host defences during the pathogenic phase, while other components initiate host cell death responses leading to the formation of necrotic tissue. The pathogenic phase is followed by a second saprophytic phase, the transition to which is effected by declining cAMP levels as glucose becomes available and further hydrolytic enzyme synthesis is repressed. Low cAMP levels and an acidic environment generated by the secretion of oxalic acid promote sclerotial development and completion of the life cycle. This review brings together histological, biochemical and molecular information gathered over the past several decades to develop this tri-phasic model for infection. In several instances, studies with Botrytis species are drawn upon for supplemental and supportive evidence for this model. In this process, we attempt to outline how the interplay between glucose levels, cAMP and ambient pH serves to coordinate the transition between these phases and dictate the biochemical and developmental events that define them.


Subject(s)
Ascomycota/pathogenicity , Oxalic Acid/metabolism , Polygalacturonase/metabolism , Ascomycota/metabolism , Oxidative Stress , Plant Diseases/microbiology , Polygalacturonase/genetics
17.
Mol Plant Microbe Interact ; 17(7): 711-9, 2004 Jul.
Article in English | MEDLINE | ID: mdl-15242165

ABSTRACT

Resistance to Albugo candida isolate Acem1 is conferred by a dominant gene, RAC1, in accession Ksk-1 of Arabidopsis thaliana. This gene was isolated by positional cloning and is a member of the Drosophila toll and mammalian interleukin-1 receptor (TIR) nucleotide-binding site leucine-rich repeat (NB-LRR) class of plant resistance genes. Strong identity of the TIR and NB domains was observed between the predicted proteins encoded by the Ksk-1 allele and the allele from an Acem1-susceptible accession Columbia (Col) (99 and 98%, respectively). However, major differences between the two predicted proteins occur within the LRR domain and mainly are confined to the beta-strand/beta-turn structure of the LRR. Both proteins contain 14 imperfect repeats. RAC1-mediated resistance was analyzed further using mutations in defense regulation, including: pad4-1, eds1-1, and NahG, in the presence of the RAC1 allele from Ksk-1. White rust resistance was completely abolished by eds1-1 but was not affected by either pad4-1 or NahG.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/genetics , Carboxylic Ester Hydrolases/genetics , DNA-Binding Proteins/genetics , Amino Acid Sequence , Arabidopsis/metabolism , Arabidopsis/microbiology , Arabidopsis Proteins/metabolism , Base Sequence , Carboxylic Ester Hydrolases/metabolism , Chromosomes, Artificial, Yeast , DNA-Binding Proteins/metabolism , Immunity, Innate/genetics , Molecular Sequence Data , Oomycetes/growth & development , Plant Diseases/genetics , Plant Diseases/microbiology , Sequence Homology, Amino Acid , Signal Transduction/genetics , Signal Transduction/physiology
18.
Phytopathology ; 93(8): 959-65, 2003 Aug.
Article in English | MEDLINE | ID: mdl-18943862

ABSTRACT

ABSTRACT The inheritance of avirulence and polymorphic molecular markers in Albugo candida, the cause of white rust of crucifers, was studied in crosses of race 2 (Ac2), using isolates MiAc2-B1 or MiAc2-B5 (metalaxyl-insensitive and virulent to Brassica juncea cv. Burgonde) with race 7 (Ac7), using isolate MsAc7-A1 (metalaxyl-sensitive and virulent to B. rapa cv. Torch). Hybrids were obtained via co-inoculation onto a common susceptible host. Putative F(1) progeny were selfed to produce F(2) progeny. The parents and F(1) progeny were examined for virulence on the differential cultivars B. juncea cv. Burgonde and B. rapa cv. Torch. Segregation of avirulence or virulence of F(2) populations was analyzed on cv. Torch. Putative F(1) hybrids were confirmed by random amplified polymorphic DNA markers specific for each parent. Avirulence or virulence of F (2) progeny to B. rapa cv. Torch suggested 3:1 in each of three populations, supporting the hypothesis of a single dominant avirulence gene. Amplified fragment length polymorphism markers also segregated in regular Mendelian fashion among F(2) progeny derived from two F(1) hybrids (Cr2-5 and Cr2-7) of Cross-2. This first putative avirulence gene in A. candida was designated AvrAc1. These results suggest that a single dominant gene controls avirulence in race Ac2 to B. rapa cv. Torch and provides further evidence for the gene-for-gene relationship in the Albugo-Brassica pathosystem.

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