Validation of Antimicrobial Interventions for Reducing Shiga Toxin-Producing Escherichia coli Surrogate Populations during Goat Slaughter and Carcass Chilling.

Demand and consumption of goat meat is increasing in the United States due to an increase in ethnic populations that prefer goat meat. As ruminant animals, goats are known reservoirs for Shiga toxin-producing Escherichia coli (STEC) and proper handling, especially during slaughter, is imperative to reduce the likelihood of carcass and meat contamination. However, the majority of antimicrobial intervention studies during the slaughter of ruminant species have focused on beef, highlighting the need for validation studies targeting small ruminants, such as goats, during slaughter and chilling procedures. The objective of this research was to evaluate 4.5% lactic acid (LA; pH 2.1), peroxyacetic acid (PAA; 400 ppm; pH 4.7), a hydrochloric and citric acid blend (Citrilow [CL]; pH 1.2), 5% levulinic acid plus 0.5% sodium dodecyl sulfate (LVA+SDS; pH 2.60), and a nontreated control (CON) for their efficacy in reducing STEC surrogates and their effect on carcass color from slaughter through 24-h chill. Fifteen goat carcasses across three replications were inoculated with a five-strain cocktail (ca. 5 log CFU/cm2 attachment), containing rifampin-resistant surrogate E. coli (BAA-1427, BAA-1428, BAA-1429, BAA-1430, and BAA-1431) and were randomly assigned to an antimicrobial treatment. Antimicrobials were applied prechill and 24 h postchill. Mean log reductions achieved after prechill treatment with LA, PAA, CL, and LVA+SDS were 2.00, 1.86, 2.26, and 1.90 log CFU/cm2, respectively. Antimicrobial treatment after the 24-h chilling, resulted in additional reductions of surrogate E. coli by 0.99, 1.03, 1.94, and 0.47 log CFU/cm2 for LA, PAA, CL, and LVA+SDS, respectively. Antimicrobial treatments did not impact goat carcass objective color (L* and a*), except for b*. The antimicrobials tested in this study were able to effectively reduce surrogate STEC populations during slaughter and subsequent chilling without compromising carcass color.

Elemental composition of HULIS in the Pearl River Delta Region, China: results inferred from positive and negative electrospray high resolution mass spectrometric data.

The HUmic-LIke Substances (HULIS) fraction isolated from aerosol samples collected at a rural location of the Pearl River Delta Region (PRD), China, during the harvest season was analyzed by both positive and negative mode electrospray ionization (ESI) coupled with an ultrahigh resolution mass spectrometer (UHRMS). With the remarkable resolving power and mass accuracy of ESI-UHRMS, thousands of elemental formulas were identified. Formulas detected in the positive (ESI+) and the negative (ESI-) mode complement each other due to differences in the ionization mechanism, and the use of both provides a more complete characterization of HULIS. Compounds composed of C, H, and O atoms were preferentially detected in ESI- by deprotonation, implying their acidic properties. Tandem MS and Kendrick Mass Defect analysis implies that carboxyl groups are abundant in the CHO compounds. This feature is similar to those of natural fulvic acids, but relatively smaller molecular weights are observed in the HULIS samples. A greater number of reduced nitrogen organic compounds were observed in the ESI+ compared to ESI-. Compounds with biomass burning origin including alkaloids, amino acids, and their derivatives are their probable constituents. Sulfur-containing species were dominantly detected in ESI-. The presence of sulfate fragments in the MS/MS spectra of these species and their high O/S ratios implies that they are mainly organosulfates. Organosulfates and nitrooxy-organosulfates were often the most intensive peaks in the ESI- spectra. They are believed to be products of reactive uptake of photooxidation products of reactive volatile organic compounds by acidic sulfate particles. The elemental compositions deduced from the UHRMS analysis confirm the conclusion from our previous study that biomass burning and SOA formation are both important sources of HULIS in the PRD region.

Optical absorptivity versus molecular composition of model organic aerosol matter.

Aerosol particles affect the Earth's energy balance by absorbing and scattering radiation according to their chemical composition, size, and shape. It is generally believed that their optical properties could be deduced from the molecular composition of the complex organic matter contained in these particles, a goal pursued by many groups via high-resolution mass spectrometry, although: (1) absorptivity is associated with structural chromophores rather than with molecular formulas, (2) compositional space is a small projection of structural space, and (3) mixtures of polar polyfunctional species usually exhibit supramolecular interactions. Here we report a suite of experiments showing that the photolysis of aqueous pyruvic acid (a proxy for aerosol alpha-dicarbonyls absorbing at lambda > 300 nm) generates mixtures of identifiable aliphatic polyfunctional oligomers that develop absorptions in the visible upon standing in the dark. These absorptions and their induced fluorescence emissions can be repeatedly bleached and retrieved without carbon loss or ostensible changes in the electrospray mass spectra of the corresponding mixtures and display unambiguous signatures of supramolecular effects. The nonlinear additivity of the properties of the components of these mixtures supports the notion that full structural speciation is insufficient and possibly unnecessary for understanding the optical properties of aerosol particles and their responses to changing ambient conditions.

Nuevos sistemaseelectroquímicos y fotoquímicos ara el tratamiento de aguas ressiduales y de bebida / New electrochmiccal and photochemical systems for water and wastewater treatment

Con la creciente presión hacia un uso más racional del recurso hídrico, el desarrollo de tecnologías apropiadas de tratamiento de aguas es cada vez más urgente. Debido a ello, en los últimos años se han propuesto procesos de oxidación fotoquímicos y electroquímicos como alternativa para el tratamiento de aguas contaminadas con sustancias antropogénicas difícilmente biodegradables, así como para eliminar bacterias en aguas destinadas al consumo humano. El objetivo de este trabajo es presentar algunas de nuestras más recientes investigaciones que demuestran que tanto la electroquímica como la fotoquímica, al igual que el acople de estos procesos con tratamientos biológicos, son alternativas muy prometedoras para aumentar la calidad del agua.

With the increasing pressure on a more effective use of water resources, the developpement of appropriate water treatment technologies become more and more important. Photochemical and electrochemical oxidation processes have been proposed in recent years as an attractive alternative for the treatment of contaminated water containing anthropogenic substances hardly biodegradable as well as to purify and disinfect drinking waters. The aim of this paper is to present some of our last results demonstrating that electrochemical, photochemical, and the coupling of these processes with biological systems are very promising alternativesfor the improvement of the water quality.