ABSTRACT
Resumen La finalidad de la presente investigación es realizar la caracterización psicosocial de personas de origen latinoamericano que delinquieron en España y que se encuentran internas en el Centro de Inserción Social de la comunidad de Andalucía en la ciudad de Málaga. Para esto, se utilizó una metodología mixta (cualitativa y cuantitativa) de tipo empírica e inductiva. En la cual se realizaron entrevistas semiestructuradas, observación participante, la aplicación de pruebas psicológicas para evaluar los constructos de autoestima e impulsividad y una encuesta. Se analizó la información en los siguientes tópicos: características sociodemográficas, dinámica cultural y social, composición familiar, salud física y psicológica, autoestima, impulsividad, motivaciones para delinquir, escalamiento de conductas análogas, antisociales y delictivas, redes de apoyo o vínculos socio familiares, factores del proceso de inserción social, reincidencia y proyecto de vida. Como resultado se obtuvieron similitudes en los factores culturales, sociales y geográficos entre otros, no obstante, se determinó que no existe un solo factor que explique la conducta delictiva.
Abstract The purpose of the present investigation is to perform psychosocial characterization of people of Latin American origin who have committed crimes in Spain and who are internal in the Center of Social Insertion of the community of Andalusia in the city of Malaga; for this, mixed (qualitative and quantitative) methodology of empirical and inductive type was used, where different tools were used to collect information, including semi-structured interviews, participant observation, the application of psychological tests to evaluate the constructs of self-esteem and impulsivity and a survey; Subsequently, the information collected on the following topics was analyzed and processed: sociodemographic characteristics, cultural and social dynamics, family composition, physical and psychological health, self-esteem, impulsivity, motivation to commit a crime, escalation of analogous, antisocial and criminal behaviors, support networks or socio - family ties, factors of the social insertion process, recidivism and life project. As a result, similarities were obtained in cultural, social and geographical factors among others, however, it was determined that there is not a single factor that explains the criminal behavior.
ABSTRACT
BACKGROUND AND OBJECTIVE: The characteristics of human hematopoietic stem cells are conditioned by the microenvironment of the bone marrow, where they interact with other cell populations, such as mesenchymal stem cells and endothelial cells; however, the study of this microenvironment is complex. The objective of this work was to develop a 3D culture system by magnetic levitation that imitates the microenvironment of human HSC. METHODS AND RESULTS: Human bone marrow-mesenchymal stem cells, umbilical cord blood-hematopoietic stem cells and a non-tumoral endothelial cell line (CC2811, Lonzaâ) were used to develop organotypic multicellular spheres by the magnetic levitation method. We obtained viable structures with an average sphericity index greater than 0.6, an average volume of 0.5 mm3 and a percentage of aggregation greater than 70%. Histological studies of the organotypic multicellular spheres used hematoxylin and eosin stains, and an evaluation of vimentin expression by means of immunohistochemistry demonstrated an organized internal structure without picnotic cells and a high expression of vimentin. The functional capacity of human hematopoietic stem cells after organotypic multicellular spheres culture was evaluated by multipotency tests, and it was demonstrated that 3D structures without exogenous Flt3L are autonomous in the maintenance of multipotency of human hematopoietic stem cells. CONCLUSIONS: We developed organotypic multicellular spheres from normal human cells that mimic the microenvironment of the human hematopoietic stem cells. These structures are the prototype for the development of complex organoids that allow the further study of the biology of normal human stem cells and their potential in regenerative medicine.
