1.
Both chloroplastic and cytosolic phosphofructoaldolase isozymes are present in the pea leaf nucleus.
Protoplasma
; 225(3-4): 235-42, 2005 Oct.
Article
in English
| MEDLINE
| ID: mdl-16228901
ABSTRACT
In higher plants, fructose bisphosphate aldolase (EC 4.1.2.13) occurs in chloroplast, cytosol, and nucleus. Immunocytolocalization experiments with isozyme-directed antibodies indicate that both chloroplastic and cytosolic aldolase isoforms are present in the pea (Pisum sativum L.) leaf nucleus.
Subject(s)
Cell Nucleus/enzymology , Chloroplasts/enzymology , Cytosol/enzymology , Fructose-Bisphosphate Aldolase/metabolism , Pisum sativum/enzymology , Chloroplasts/ultrastructure , Electrophoresis, Polyacrylamide Gel , Immunoblotting , Isoenzymes/metabolism , Pisum sativum/cytology , Plant Leaves/cytology , Plant Leaves/enzymology
2.
Protoplasma
; 223(1): 33-43, 2004 Mar.
Article
in English
| MEDLINE
| ID: mdl-15004741
ABSTRACT
Immunolocalization experiments indicate that both the subunit B of the NADP-linked chloroplastic glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) and the NAD-linked cytosolic enzyme (EC 1.2.1.12) are present in the pea ( Pisum sativum L.) leaf nucleus. Subunit A of the NADP-linked enzyme appears to be restricted to the chloroplast.