ABSTRACT
A sensitive method is described for the measurement of d-glaucine in pharmacokinetic studies using only 100-microliter plasma samples or 200-microliter urine samples. It requires a simple extraction clean-up on kieselguhr micro-columns and straight-phase high-performance liquid chromatography with fluorescence detection. Data on selectivity, sensitivity and precision demonstrate the reliability of this method. Its applicability is revealed by single and repeated oral administration pharmacokinetic studies in human subjects.
Subject(s)
Aporphines/analysis , Administration, Oral , Aporphines/metabolism , Chromatography, High Pressure Liquid/methods , Humans , KineticsABSTRACT
N-Desmethyldiazepam in plasma has been determined by gas chromatography with electron capture detection, using N-desmethyltetrazepam as internal standard and a column filled Poly-A 103 on Gas-Chrom Q. No prior transformation of benzodiazepines is necessary. The sensitivity is ca.1 ng/ml and the recovery is greater than or equal to 70%. The method is particularly suitable for the pharmacokinetic study of dipotassium clorazepate: the amount of unchanged clorazepate may be estimated from the difference between the amount of N-desmethyldiazepam present initially and the total amount of N-desmethyldiazepam obtained after acidic treatment of the sample; the method is also useful for therapeutic control and for analytical toxicology.
