Phenotype-fitness relationships and pollen-transfer efficiency of five orchid species with different pollination strategies.

PREMISE: Deceptive pollination, a fascinating mechanism that independently originated in several plant families for benefiting from pollinators without providing any reward, is particularly widespread among orchids. Pollination efficiency is crucial in orchids due to the aggregated pollen in a pollinarium, which facilitates pollen transfer and promotes cross-pollination as pollinators leave after being deceived. METHODS: In this study, we compiled data on reproductive ecology from five orchid species with different pollination strategies: three deceptive-strategy species (shelter imitation, food deception, sexual deception), one nectar-rewarding species, and one shelter-imitation but spontaneously selfing species. We aimed to compare the reproductive success (female fitness: fruit set; male fitness: pollinarium removal) and pollination efficiency of species representing these strategies. We also investigated pollen limitation and inbreeding depression among the pollination strategies. RESULTS: Male and female fitness were strongly correlated in all species but the spontaneously selfing species, which had high fruit set and low pollinarium removal. As expected, pollination efficiency was highest for the rewarding species and the sexually deceptive species. Rewarding species had no pollen limitation but did have high cumulative inbreeding depression; deceptive species had high pollen limitation and moderate inbreeding depression; and spontaneously selfing species did not have pollen limitation or inbreeding depression. CONCLUSIONS: Pollinator response to deception is critical to maintain reproductive success and avoid inbreeding in orchid species with non-rewarding pollination strategies. Our findings contribute to a better understanding of the trade-offs associated with different pollination strategies in orchids and highlight the importance of pollination efficiency in orchids due to the pollinarium.

Climate change and their impacts in the Balearic Islands: a guide for policy design in Mediterranean regions.

Understanding the local effects of global warming-derived impacts is important to island systems due to their fragile environmental conditions. This is especially true when it comes to Mediterranean insular regions as they are climate change (CC) hotspots where adaptation and mitigation policy design is an urgent matter. Looking at 2030 as a time horizon for climate action and focusing on the Balearic Islands, this paper reviews the physical changes projected for the coming decades as a result of CC and analyses their impacts on regional environmental, economic and social variables. Mitigation and adaptation measures are also proposed based on the identified priority impacts. The fact the Balearics are a top world holiday destination allows the analysis to serve as a guide to other Mediterranean islands with tourism-based economies facing similar CC scenarios. Results show the projected rise of temperature and sea level; the reduction of the average precipitation and increase in evapotranspiration, the droughts and the increase in ocean acidification and deoxygenation are the main threats faced by the Balearics, this putting their economy at risk due to the high tourism's vulnerability to CC. Mitigation and adaptation action on terrestrial and marine ecosystems, water resources, energy, infrastructure and urban planning, human health, economy, law and education is recommended. Sustainable mobility and waste managing are also viewed as important fields for mitigation action. Conclusions show that diversifying the current socioeconomic model is needed to increase the community and territory resilience. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10113-021-01810-1.

Historic exposure to herbivores, not constitutive traits, explains plant tolerance to herbivory in the case of two Medicago species (Fabaceae).

Mechanisms that allow plants to survive and reproduce after herbivory are considered to play a key role in plant evolution. In this study, we evaluated how tolerance varies in species with different historic exposure to herbivores considering ontogeny. We exposed the range-restricted species Medicago citrina and its closely related and widespread species M. arborea to one and two herbivory simulations (80 % aerial biomass loss). Physiological and growth parameters related to tolerance capacity were assessed to evaluate constitutive values (without herbivory) and induced tolerance after damage. Constitutive traits were not always related to greater tolerance, and each species compensated for herbivory through different traits. Herbivory damage only led to mortality in M. citrina; adults exhibited root biomass loss and increased oxidative stress after damage, but also compensated aerial biomass. Despite seedlings showed a lower death percentage than adults after herbivory in M. citrina, they showed less capacity to recover control values than adults. Moderate tolerance to M. arborea herbivory and low tolerance to M. citrina is found. Thus, although the constitutive characteristics are maintained in the lineage, the tolerance of plants decreases in M. citrina. That represents how plants respond to the lack of pressure from herbivores in their habitat.

The role of landscape composition and heterogeneity on the taxonomical and functional diversity of Mediterranean plant communities in agricultural landscapes.

The expansion of agriculture is a major driver of biodiversity loss worldwide, through changes generated in the landscape. Despite this, very little is still known about the complex relationships between landscape composition and heterogeneity and plant taxonomical and functional diversity in Mediterranean ecosystems that have been extensively managed during millennia. Although according to the Intermediate Disturbance Hypothesis (IDH) plant richness might peak at intermediate disturbance levels, functional diversity might increase with landscape heterogeneity and decrease with the intensity of disturbance. Here, we evaluated the associations of landscape composition (percentage of crops) and heterogeneity (diversity of land-cover classes) with plant taxonomical diversity (richness, diversity, evenness), local contribution to beta diversity, and functional diversity (functional richness, evenness, divergence and dispersion) in 20 wild Olea europaea communities appearing within agricultural landscapes of Mallorca Island (Western Mediterranean Basin). In accordance with the IDH, we found that overall plant richness peaked at intermediate levels of crops in the landscape, whereas plant evenness showed the opposite pattern, because richness peak was mainly related to an increase in scarce ruderal species. Plant communities surrounded by very heterogeneous landscapes were those contributing the most to beta diversity and showing the highest functional richness and evenness, likely because diverse landscapes favour the colonization of new species and traits into the communities. In addition, landscape heterogeneity decreased functional divergence (i.e., increased trait overlap of dominant species) which may enhance community resilience against disturbances through a higher functional redundancy. However, a large extent of agriculture in the landscape might reduce such resilience, as this disturbance acted as an environmental filter that decreased functional dispersion (i.e, remaining species shared similar traits). Overall, our study highlights the importance of considering several indices of taxonomical and functional diversity to deeply understand the complex relationships between ecosystems functions and landscape context.

The correct structure of aquatolide-experimental validation of a theoretically-predicted structural revision.

Aquatolide has been reisolated from its natural source, and its structure has been revised on the basis of quantum-chemical NMR calculations, extensive experimental NMR analysis, and crystallography.