Subject(s)
Hemangioendothelioma, Epithelioid/pathology , Lip Neoplasms/pathology , Sarcoma/pathology , Skin Neoplasms/pathology , Adult , Biomarkers, Tumor/analysis , Biopsy , Chin , Hemangioendothelioma, Epithelioid/chemistry , Hemangioendothelioma, Epithelioid/surgery , Humans , Immunohistochemistry , Lip Neoplasms/chemistry , Lip Neoplasms/surgery , Male , Sarcoma/chemistry , Sarcoma/surgery , Skin Neoplasms/chemistry , Skin Neoplasms/surgeryABSTRACT
Human skin undergoes morphological and biochemical changes as a result of chronological aging and exposure to solar ultraviolet irradiation (photoaging). Noninvasive detection of these changes may aid in the prevention and treatment of both types of aging. This article presents a noninvasive method for the evaluation of aging skin with a unilateral stray field NMR scanner. These portable and inexpensive scanners may be suitable for in-depth skin characterization. In vivo profiles of sun-protected and sun-exposed skin from the forearms of female subjects of different ages (n = 9) were measured. Skin biopsies for histopathological examination were used as reference. T2 analysis with a bi-exponential decay model was applied and the extracted parameters were examined as markers for dermal aging. In the upper reticular dermis, a significant increase in the fraction of the slow T2 component and in the T2 value itself was found to correlate with chronological aging. For most subjects, there was an additional increase in the values of the slow T2 component and the T2 values from the sun-exposed forearm, superimposed on that measured for the sun-protected forearm. These results are in agreement with the decline in collagen content and the increase in free water content with aging. The results suggest that such a technique can be used as a tool for the assessment of aging, and that bi-exponential fitting can produce sensitive fingerprint parameters for the dermal alterations that occur during aging.
Subject(s)
Magnetic Resonance Spectroscopy/instrumentation , Skin Aging/physiology , Skin Aging/radiation effects , Skin/chemistry , Skin/radiation effects , Sunlight , Adult , Aged , Aged, 80 and over , Aging/physiology , Computer Simulation , Equipment Design , Equipment Failure Analysis , Female , Humans , Magnetic Resonance Spectroscopy/methods , Middle Aged , Models, Biological , Reproducibility of Results , Sensitivity and Specificity , Young AdultABSTRACT
T-cell receptor gene rearrangement studies are considered to be an adjunct to the histopathological diagnosis of mycosis fungoides (MF). Fluorophore-coupled primers in polymerase chain reactions followed by fragment analysis with a capillary electrophoresis device (GeneScan analysis) have been recently advocated and widely used. This study was performed to assess T-cell receptor γ-gene rearrangement GeneScan as an adjunct to the histopathological diagnosis of MF. We studied 163 cases. The rates of clonality using this technique were found to be 22/37 (59.5%) in early patch MF, 30/46 (65.2%) in more advanced stages, 13/34 (38.2%) in inconclusive cases, and 10/46 (21.7%) in benign inflammatory dermatoses. Our results support the histopathological criteria for the diagnosis of early patch MF, because of the close rates of clonality obtained in early patch MF with subtle histopathological findings, and in the more advanced subtypes of MF with more overt and specific histopathological criteria.
