ABSTRACT
Two-month old piglets previously inoculated with different dilutions of the Lapinized Chinese (LC) strain of Hog Cholera Virus (HCV), adapted in a minipig kidney (MPK) cell line, resisted challenge infection with virulent HCV. All the animals remained healthy and the challenge virus was never recovered from any of them. In contrast, the pigs which served as controls for the challenging virus underwent the clinically lethal form of the disease and HCV was consistently recovered from their tissues.
Subject(s)
Classical Swine Fever Virus/immunology , Classical Swine Fever/prevention & control , Viral Vaccines , Animals , Antibodies, Viral/biosynthesis , Cell Line , Classical Swine Fever Virus/pathogenicity , Swine , Swine, Miniature , VirulenceABSTRACT
The susceptibility of two established cell lines of pig (MPK = minipig kidney) and rabbit (RK13 = rabbit kidney) origin to the lapinized Chinese (LC) strain of hog cholera virus (HCV) was studied. Spleen cells from rabbits infected with the virus under study were inoculated to cell cultures of either MPK and RK13 cells and subsequent passages were made by culturing the trypsinized infected cells with the normal cells. Only the MPK cell line appeared to be susceptible to virus replication. Since no cytopathic effects (CPE) were observed, the presence of the viral antigen in the inoculated cultures was detected by immunofluorescence tests. The virulence of the virus for rabbits was enhanced after its cultivation in MPK cell cultures. When the MPK cell culture system adapted virus was tested in neutralization trials in the presence of an HCV reference immune serum it was found that the virus did not modify its antigenic structure in any extent. Finally, the culture adapted virus appeared to be more immunogenic for rabbits than the original rabbits adapted virus. Based on these results, it seems reasonable to suggest the use of MPK cell line for the propagation of the LC strain of HCV as an alternative to the use of rabbits for the preparation of HCV vaccine.
Subject(s)
Classical Swine Fever Virus/physiology , Animals , Antigens, Viral/analysis , Cell Line , Classical Swine Fever Virus/growth & development , Classical Swine Fever Virus/immunology , Cytopathogenic Effect, Viral , Fluorescent Antibody Technique , Rabbits , Spleen/microbiology , Swine , Swine, Miniature , Virus ReplicationABSTRACT
A comparitive study on the different susceptibility of MPK cells (Minipig Kidney cell line) and PK15 cells (Pig Kidney cell line) to the Hog Cholera Virus (HCV) was conducted. Higher HCV titres (3 log10) were reached on MPK cells compared with PK15 cells.
