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1.
World J Surg Oncol ; 20(1): 100, 2022 Mar 30.
Article in English | MEDLINE | ID: mdl-35354485

ABSTRACT

BACKGROUND AND AIMS: Recent studies focusing on thoracic surgery suggest postoperative kidney injury depending on the amount of perioperative blood transfusions. Data investigating similar effects after resection of colorectal liver metastases (CRLM) are not available. Aim of this study was therefore to evaluate the influence of perioperative blood transfusions on postoperative renal function and survival after resection of CRLM. METHODS: Seven hundred twenty-seven cases of liver resection for CRLM were retrospectively analyzed. Renal function was measured via estimated glomerular filtration rate (eGFR) and a postoperative decline of ≥ 10% was considered substantial. Potential influences on postoperative kidney function were assessed using univariable and multivariable logistic regression analyses. Cox-regression analyses were performed to estimate the impact on overall survival (OS). RESULTS: Preoperative impaired kidney function (p = 0.001, OR 2.477) and transfusion of > 2 units of packed red blood cells (PRBC) (p = 0.046; OR 1.638) were independently associated with an increased risk for ≥ 10% loss of renal function. Neither a pre-existing renal impairment, nor the additional loss of renal function were associated with reduced survival. Chemotherapies in the context of primary colorectal cancer treatment (p = 0.002), age > 70 years at liver resection (p = 0.005), number (p = 0.001), and size of metastases > 50 mm (p = 0.018), duration of resection > 120 min (p = 0.006) and transfusions of > 2 units of PRBC (p = 0.039) showed a negative independent influence on OS. CONCLUSION: The results demonstrate a negative impact of perioperative blood transfusions on the postoperative renal function and OS. Hence, efforts to reduce blood transfusions should be intensified.


Subject(s)
Colorectal Neoplasms , Liver Neoplasms , Aged , Blood Transfusion , Colorectal Neoplasms/pathology , Colorectal Neoplasms/surgery , Humans , Kidney/pathology , Kidney/physiology , Liver Neoplasms/secondary , Retrospective Studies
2.
Braz J Microbiol ; 50(2): 405-413, 2019 Apr.
Article in English | MEDLINE | ID: mdl-30827000

ABSTRACT

The effect of gene dosage on the production of Candida antarctica lipase B (CalB) in the methylotrophic yeast Komagataella phaffii, at high densities in a simple medium containing crude glycerin as the sole carbon source, is described. The use of crude glycerin, the main by-product of biodiesel production from vegetable oils, will reduce the production cost of the bioprocess. Two K. phaffii strains were constructed with one or three copies of LipB, an optimized version of the gene encoding CalB under the control of the constitutive PPGK1 promoter. These two constructs were tested and compared on batches using minimal-salts medium with crude glycerin. The strain with three copies achieved a higher enzyme yield (48,760 U/L, 2.3-fold higher than the one-copy strain), with 42 g/L biomass, with no effects on growth.


Subject(s)
Candida/enzymology , Candida/genetics , Fungal Proteins/biosynthesis , Fungal Proteins/genetics , Lipase/biosynthesis , Lipase/genetics , Pichia/genetics , Saccharomycetales/metabolism , Candida/metabolism , Gene Dosage/genetics , Glycerol/metabolism , Promoter Regions, Genetic/genetics , Saccharomycetales/genetics
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