Spatial analysis of mercury and stable isotopes in the vulnerable Dusky Grouper Epinephelus marginatus along the Brazilian coast.

Mercury (Hg) is a contaminant of global concern due to its damaging toxicological effects on organisms. For the vulnerable Dusky Grouper (Epinephelus marginatus) off the coast of Brazil, we investigated: i) spatial patterns in muscle tissue total mercury (THg) contamination; ii) the relationship between muscle THg concentrations and total length iii) the relationship between muscle THg and stable isotopes; and iv) THg concentrations among muscle, liver, and ovary tissues. Out of 134 fish sampled, 21.8 % were higher than 0.5 mg/kg wet weight (above the safe limit for human consumption). THg concentrations increased toward lower latitudes, but an opposite pattern was observed for δ13C and δ15N with decreased values toward lower latitudes. There were significant differences in THg concentration among the three tissues. Results of Hg concentrations are useful for understanding the potential adverse effects on the health of this vulnerable species and to serve as a guide to human consumers.

Transcriptomic profiling of miR-203a inhibitor and miR-34b-injected zebrafish (Danio rerio) validates oil-induced neurological, cardiovascular and eye toxicity response pathways.

The global sequencing of microRNA (miRNA; miR) and integration to downstream mRNA expression profiles in early life stages (ELS) of fish following exposure to crude oil determined consistently dysregulated miRNAs regardless of the oil source or fish species. The overlay of differentially expressed miRNAs and mRNAs into in silico software determined that the key roles of these miRNAs were predicted to be involved in cardiovascular, neurological and visually-mediated pathways. Of these, altered expression of miRNAs, miR-203a and miR-34b were predicted to be primary targets of crude oil. To better characterize the effect of these miRNAs to downstream transcript changes, zebrafish embryos were microinjected at 1 h post fertilization (hpf) with either a miR-203a inhibitor or miR-34b. Since both miRs have been shown to be associated with aryl hydrocarbon receptor (AhR) function, benzo(a)pyrene (BaP), a potent AhR agonist, was used as a potential positive control. Transcriptomic profiling was conducted on injected and exposed larvae at 7 and 72 hpf, and eye morphology assessed following exposure at 72 hpf. The top predicted physiological system disease and functions between differentially expressed genes (DEGs) shared with miR-203a inhibitor-injected and miR-34b-injected embryos were involved in brain formation, and the development of the central nervous system and neurons. When DEGs of miR-203a inhibitor-injected embryos were compared with BaP-exposed DEGs, alterations in nervous system development and function, and abnormal morphology of the neurosensory retina, eye and nervous tissue were predicted, consistent with both AhR and non-AhR pathways. When assessed morphologically, the eye area of miR-203a inhibitor and miR-34b-injected and BaP-exposed embryos were significantly reduced. These results suggest that miR-203a inhibition and miR-34b overexpression contribute to neurological, cardiovascular and eye toxicity responses that are caused by oil and PAH exposure in ELS fish, and are likely mediated through both AhR and non-AhR pathways.

Maritime autonomous surface ships: can we learn from unmanned aerial vehicle incidents using the perceptual cycle model?

Interest in Maritime Autonomous Surface Ships (MASS) is increasing as it is predicted that they can bring improved safety, performance and operational capabilities. However, their introduction is associated with a number of enduring Human Factors challenges (e.g. difficulties monitoring automated systems) for human operators, with their 'remoteness' in shore-side control centres exacerbating issues. This paper aims to investigate underlying decision-making processes of operators of uncrewed vehicles using the theoretical foundation of the Perceptual Cycle Model (PCM). A case study of an Unmanned Aerial Vehicle (UAV) accident has been chosen as it bears similarities to the operation of MASS through means of a ground-based control centre. Two PCMs were developed; one to demonstrate what actually happened and one to demonstrate what should have happened. Comparing the models demonstrates the importance of operator situational awareness, clearly defined operator roles, training and interface design in making decisions when operating from remote control centres. Practitioner Summary: To investigate underlying decision-making processes of operators of uncrewed vehicles using the Perceptual Cycle Model, by using an UAV accident case study. The findings showed the importance of operator situational awareness, clearly defined operator roles, training and interface design in making decisions when monitoring uncrewed systems from remote control centres.

Persistent organic pollutant exposure and associations with gene expression in northern fur seals (Callorhinus ursinus) from St. Paul Island, Alaska.

Persistent organic pollutants (POPs) are highly lipophilic compounds that accumulate at increased concentrations in high tropic level organisms like marine mammals. Marine mammals' reliance on blubber makes them susceptible to accumulating POPs at potentially toxic concentrations. In this study, we analyzed POP concentrations, (polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), polybrominated diphenyl ethers (PBDEs), and methoxylated-BDE (MeOBDE), in the blubber of 16 subsistence harvested sub-adult, male northern fur seals as well as assessed changes in mRNA gene expression of nine relevant biomarkers including the aryl hydrocarbon receptor, thyroid receptor-α, and adiponectin. PBDE and MeOBDE concentrations were significantly lower than PCB and OCP concentrations. A negative relationship was observed between percent lipid in the blubber and contaminant concentrations, both individual and sum. Expression changes in eight biomarkers were correlated with individual and sum contaminant concentrations. This study shows that contaminant concentrations measured are correlated to changes in expression of genes from different physiological systems, metabolism and endocrine, that are important for the regulation of blubber metabolism. Northern fur seals are reliant on blubber as an energy source during times of low food intake. Potential contaminant induced changes in blubber metabolism pathways could have significant impacts on the health of individuals during critical periods.

Transcriptomic responses and apoptosis in larval red drum (Sciaenops ocellatus) co-exposed to crude oil and ultraviolet (UV) radiation.

Ultraviolet (UV) radiation can significantly increase the toxicity of polycyclic aromatic hydrocarbons (PAHs) in crude oil to early life stage (ELS) fishes through photo-induced /photo-enhanced toxicity. However, little is known about the sub-lethal effects and mechanisms of photo-induced PAH toxicity in ELS fishes. The present study investigated apoptosis and global transcriptomic effects in larval red drum (Sciaenops ocellatus) (24-72 h post-fertilization) following co-exposure to oil (0.29-0.30 µg/L ∑PAH50) and UV. Apoptosis was quantified using the TUNEL assay, and transcriptomic effects were assessed using RNA sequencing analysis. Apoptotic fluorescence was greatest in the eyes and skin following 24 and 48 h co-exposure to oil and UV, indicating photo-induced toxicity. Consistent with these phenotypic responses, pathways associated with phototransduction, eye development, and dermatological disease were among the top predicted pathways impacted. The present study is the first to provide global transcriptomic analysis of UV and oil co-exposure in an ELS fish.

State of science: models and methods for understanding and enhancing teams and teamwork in complex sociotechnical systems.

This state of the science review brings together the disparate literature of effective strategies for enhancing and accelerating team performance. The review evaluates and synthesises models and proposes recommended avenues for future research. The two major models of the Input-Mediator-Output-Input (IMOI) framework and the Big Five dimensions of teamwork were reviewed and both will need significant development for application to future teams comprising non-human agents. Research suggests that a multi-method approach is appropriate for team measurements, such as the integration of methods from self-report, observer ratings, event-based measurement and automated recordings. Simulations are recommended as the most effective team-based training interventions. The impact of new technology and autonomous agents is discussed with respect to the changing nature of teamwork. In particular, whether existing teamwork models and measures are suitable to support the design, operation and evaluation of human-nonhuman teams of the future. Practitioner summary: This review recommends a multi-method approach to the measurement and evaluation of teamwork. Team models will need to be adapted to describe interaction with non-human agents, which is what the future is most likely to hold. The most effective team training interventions use simulation-based approaches.

Exposure of zebrafish larvae to water accommodated fractions of weathered crude oil alters steroid hormone concentrations with minimal effect on cholesterol.

Crude oil has multiple toxic effects in fish, particularly during their early life stages. Recent transcriptomics studies have highlighted a potential effect on cholesterol homeostasis and biosynthesis, but have not investigated effects on steroid hormones, which are biosynthetically downstream metabolites of cholesterol. We exposed zebrafish (Danio rerio) embryos and larvae to 3 concentrations of a high energy water accommodated fraction (HEWAF) of crude oil and measured effects on cholesterol and steroid hormones at 48 and 96 h post fertilization (hpf). HEWAF exposure caused a small decrease in cholesterol at 96 hpf but not 48 hpf. HEWAF-exposed larvae had higher levels of androstenedione, testosterone, estradiol, cortisol, corticosterone, and progesterone at 96 hpf compared to controls, while effects at 48 hpf were more modest or not present. 2-Methoxyestradiol was lower following HEWAF exposure at both time points. Dihydrotestosterone was elevated in one HEWAF concentration at 48 hpf only. Our results suggest that hormone imbalance may be an important toxic effect of oil HEWAF exposure despite no major effect on their biosynthetic precursor cholesterol.

The quest for the ring: a case study of a new submarine control room configuration.

Submarine control room layouts have remained similar across decades of operation, despite the introduction of new technologies that allow for the co-location of the sound and control room. Operation of an inwards, rather than outwards, facing ring control room configuration was examined in three scenarios: Return to Periscope Depth, Inshore Operations, and Dived Tracking. A case study design employed a serving team of qualified submariners participated in all three scenarios with high and low demand. Communications and activities in the control room were recorded and analysed using the Event Analysis of Systemic Teamwork (EAST) method. EAST models collaborative teamwork through social, information, and task networks. The results from the ring configuration were compared to an outward facing baseline of the contemporary control room layout with a separate sound and control room. The ring control room configuration increased communications between operators, leading to a more information exchanged, and more tasks completed. Practitioner summary: Control room design on submarines, and other domains, has traditionally been outward facing with supervisory staff looking over the shoulders of their subordinates. In this paper, and inward looking control room design was explored, with subordinate staff facing their supervisors. This design resulted in more information exchange and productive work.

A review of the toxicology of oil in vertebrates: what we have learned following the Deepwater Horizon oil spill.

In the wake of the Deepwater Horizon (DWH) oil spill, a number of government agencies, academic institutions, consultants, and nonprofit organizations conducted lab- and field-based research to understand the toxic effects of the oil. Lab testing was performed with a variety of fish, birds, turtles, and vertebrate cell lines (as well as invertebrates); field biologists conducted observations on fish, birds, turtles, and marine mammals; and epidemiologists carried out observational studies in humans. Eight years after the spill, scientists and resource managers held a workshop to summarize the similarities and differences in the effects of DWH oil on vertebrate taxa and to identify remaining gaps in our understanding of oil toxicity in wildlife and humans, building upon the cross-taxonomic synthesis initiated during the Natural Resource Damage Assessment. Across the studies, consistency was found in the types of toxic response observed in the different organisms. Impairment of stress responses and adrenal gland function, cardiotoxicity, immune system dysfunction, disruption of blood cells and their function, effects on locomotion, and oxidative damage were observed across taxa. This consistency suggests conservation in the mechanisms of action and disease pathogenesis. From a toxicological perspective, a logical progression of impacts was noted: from molecular and cellular effects that manifest as organ dysfunction, to systemic effects that compromise fitness, growth, reproductive potential, and survival. From a clinical perspective, adverse health effects from DWH oil spill exposure formed a suite of signs/symptomatic responses that at the highest doses/concentrations resulted in multi-organ system failure.

To utilize automation or not to utilize automation, that is the question: An evaluation of how drills and procedures impact optronics mast usage from a sociotechnical systems perspective.

The delegation of tasks to a non-human agent in a sociotechnical system can extend human capabilities and performance. Effective performance is, however, reliant on a successful relationship between human operators and automation. Optronics is a partially automated system which has replaced periscope on board some modern submarine platforms, operating modes permit the completion of tasks either manually or utilizing automation. A reluctance to utilize automated functionality within the optronics system has been due to operator familiarity with legacy manual procedures based upon the use of a physical periscope. This highlights the gap that is prevalent between innovation, design, training and governance of automation utilization. The current work examined current (Control group) utilization of optronics technology using an expert population in a high fidelity simulator. Findings were utilized to guide the development of novel optronics specific standard operating procedures (Intervention group). Results indicate that automaton disuse was greatly reduced, which had had a positive overall impact on overall system performance with regard to productivity and accuracy. The current work highlights the importance of incorporating governance of use and training as part of an automation design and implementation program is critical to help 'maximize what you have'.

It's a circular argument: Examining how a novel configuration impacts information flow in submarine control rooms.

The continuing advancement of technology means that sociotechnical systems are primed for revolutionary changes to ways of working that can increase capability. It is critical to consider the unintended impact technology can have on human operators particularly regarding information flow and interactions within teams. Previous research revealed that the co-location of operator's dependent on each other for task relevant information can optimise information flow previously constrained by engineering considerations. The current work compared a novel circular configuration to that of a contemporary submarine control room. In the circular configuration, consoles faced inwards, permitting eye contact between operators, and three large screen displays were introduced to provide all operators with the same information. Ten teams participated in low and high demand dived tracking scenarios in a simulated submarine control room. All communications between operators were recorded in order to generate social, information, and task networks. These were statistically compared to networks generated from a baseline study of contemporary operation. Overall, the volume of verbal communications significantly reduced, information exchange was more structured, and the volume of tasks completed by operators significantly increased when operating in an inward facing circle configuration. The current work provides support for a data driven evidence-based approach to design that is information centric but endorsed by the end user to optimise performance and increase productivity. Implications of the work and future research ideas are discussed.

Effects of Dissolved Organic Carbon, Ultraviolet Light and their Co-Exposure on Deepwater Horizon crude oil acute toxicity to larval red drum (Sciaenops ocellatus).

In the aquatic environment, ubiquitous natural factors such as ultraviolet light (UV) and dissolved organic carbon (DOC) are likely to influence crude oil toxicity. The present study examined the interactive effects of DOC, UV, and DOC-UV co-exposure on the acute toxicity of Deepwater Horizon crude oil in larval red drum (Sciaenops ocellatus). Although DOC alone did not influence crude oil toxicity, it mildly reduced UV photo-enhanced toxicity. Environ Toxicol Chem 2020;39:2509-2515. © 2020 SETAC.

Planktonic Fiddler Crab (Uca longisignalis) Are Susceptible to Photoinduced Toxicity Following in ovo Exposure in Oiled Mesocosms.

Benthic organisms may be exposed to polycyclic aromatic hydrocarbons (PAHs) in marine sediments as the result of oil spills. PAH photoinduced toxicity, which has been documented in a wide range of early life stage (ELS) aquatic biota, is a phenomenon by which ultraviolet (UV) radiation potentiates the toxicity of photodynamic PAHs (often leading to mortality). Fiddler crabs (Uca longisignalis) are important ecosystem engineers that influence biogeochemical cycles via burrowing. As gravid females burrow, their eggs may bioaccumulate PAHs from contaminated sediments, leading to in ovo exposure. Consequently, free-swimming larvae exposed to intense UV may be at risk for photoinduced toxicity. In the present study, mature fiddler crabs were bred on oiled sediments contaminated via simulated tidal flux. Gravid females were transferred to clean water after 10 days, and larvae were collected at hatch. While in ovo exposures to oil alone did not affect survival, offspring that were subsequently exposed to full spectrum sunlight in clean water experienced significant mortality that corresponded with in ovo exposures to sediments containing ≥1455 µg/kg tPAH50. Results presented here provide evidence for the potential of photoinduced toxicity to occur in benthic organisms with free-swimming early life stages.

Exposure to Crude Oil Induces Retinal Apoptosis and Impairs Visual Function in Fish.

Polycyclic aromatic hydrocarbons (PAHs) present in crude oil are known to impair visual development in fish. However, the underlying mechanism of PAH-induced toxicity to the visual system of fish is not understood. Embryonic zebrafish (Danio rerio) at 4 h post fertilization were exposed to weathered crude oil and assessed for visual function using an optokinetic response, with subsequent samples taken for immunohistochemistry and gene expression analysis. Cardiotoxicity was also assessed by measuring the heart rate, stroke volume, and cardiac output, as cardiac performance has been proposed to be a contributing factor to eye-associated malformations following oil exposure. Larvae exposed to the highest concentrations of crude oil (89.8 µg/L) exhibited an increased occurrence of bradycardia, though no changes in stroke volume or cardiac output were observed. However, genes important in eye development and phototransduction were downregulated in oil-exposed larvae, with an increased occurrence of cellular apoptosis, reduced neuronal connection, and reduced optokinetic behavioral response in zebrafish larvae.

Gill filament permeabilization: A novel approach to assess mitochondrial function in sheepshead minnows (Cyprinodon variegatus) following anthraquinone exposure.

Anthracene is a highly toxic polycyclic aromatic hydrocarbon (PAH), and its toxicity is increased 8-fold after compounding exposure to UV radiation. Exposure to either the parent or photo-modified compound has been shown to cause increases in reactive oxygen species (ROS) production and lipid peroxidation. Since the majority of ROS production occurs within mitochondria, we investigated simultaneous mitochondrial respiration and ROS production in the gills of sheepshead minnows (Cyprinodon variegatus) acutely (48 h) exposed to anthraquinone (40 µg l-1). Anthraquinone exposure caused a 25% increase in oxidative phosphorylation with electrons donated to Complex I (OXPHOSCI) and a 33% increase in Leak respiration with oligomycin (Leak-OmyCI). ROS production was slightly increased (33.3%) in Leak state with oligomyocin respiring on Complex I substrates (Leak-OmyCI) after anthraquinone exposure, but this value remained unchanged in all other respiratory states. When ROS production was normalized to mitochondrial oxygen consumption, we found that ROS production was decreased in all respiratory states, but most noticeably in the Leak state. We speculate that differences in the antioxidant defense system may have played a role in decreased ROS production. Overall, in this paper we present a novel technique to measure mitochondrial function in the gill filaments of teleost fish exposed to xenobiotic molecules, and we show anthraquinone exposure alters aspects of oxidative phosphorylation and ROS production.

Better together? Investigating new control room configurations and reduced crew size in submarine command and control.

The separation of the sound and control rooms in Royal Navy submarines seems to be artefactually reducing the effectiveness of information transition and the overall productivity of the team. A proposed integrated sound and control room was tested in three scenarios: Return to Periscope Depth (RTPD), Inshore Operations (INSO) and Dived Tracking (DT). The activities and communications of a team of serving submariners were recorded in a control room, in a single case study design, comparing co-location and reduced crewing with a baseline of the separate sound and control room configurations that is representative of current submarines. The Event Analysis of Systemic Teamwork (EAST) method was used to examine changes in social, information and task networks. In general terms, the co-location of the submariner team led to more efficient communication and completion of tasks. Reducing the crew was more challenging in the higher demand scenarios. Practitioner Summary: There are constraints acting on control rooms, both in terms of physical space and crew size. This study compared conventional control room with co-location and reduced crew in turn. Teamwork improved in the collocated control room but the reduced crew struggled most under conditions of high demand. Abbreviations: DT: dived tracking; EAST: event analysis for systemic teamwork; H: high; INSO: inshore operations; L: low; OOW: office of the watch; OpsO: operations officer; Peri: periscope operator; RTPD: return to periscope depth; RN: royal navy; SoC: sonar controller; SoP: sonar operator; TMA: time-motion analyst.

The effects of team co-location and reduced crewing on team communication characteristics.

The manner in which control rooms are configured can impact the flow of information between command teams. Previous research revealed bottlenecks of communications between the Sonar Controller (SOC) and the Operations Officer (OPSO) in submarine control rooms. One way to relieve such bottlenecks is to co-locate operators reliant on one another for task relevant information. The aim of the current studies was to use multiple command teams to empirically examine a novel submarine control room configuration and a reduced crew size in comparison to a baseline of contemporary operations to see if such bottlenecks could be removed. Ten teams performed high and low demand Dived Tracking (DT) scenarios in a simulated submarine control room. Activities and communications of the teams were recorded and quantified using the Event Analysis of Systemic Teamwork (EAST) method affording statistical comparisons with a baseline condition of contemporary operations. The findings showed that the co-location of operators relieved the bottleneck of communications between the SOC and the OPSO. Although overall communications increased, this was more balanced across the team and was more adaptive to scenario demand. This was coupled with a significant increase in task completion, even with a reduced crew size, suggesting greater efficiency and productivity. Future research should seek to validate the changes observed with objective measures of task performance.

Alterations to the vision-associated transcriptome of zebrafish (Danio rerio) following developmental norethindrone exposure.

Synthetic sex steroids, like the synthetic progestin norethindrone (NET), can affect a wide variety of biological processes via highly conserved mechanisms. NET is prevalent in surface waters, yet the sub-lethal effects of NET exposure are not are net yet well characterized in aquatic biota. A few targeted gene expression and behavioral studies have concluded that NET affects the vision of adult fish; however, early life stage (ELS) fish are often more sensitive to contaminants. Furthermore, many species of fish rely heavily on visual perception for survival during development. The goal of the present study was to characterize the effects of developmental exposure to environmentally relevant concentrations of NET on the visual system of ELS zebrafish, using transcriptomics and histological methods. Results indicate that exposure to relatively low levels of NET in aquatic systems may be sufficient to affect the visual function of developing fish.

Correction to: Mercury accumulation, speciation, and temporal trends in Atlantic Stingrays (Hypanus sabinus).

The corrections are: In the 2nd sentence of the Statistical Analyses section, the current sentence lacks important statistical assumption wording.

Mahi-mahi (Coryphaena hippurus) life development: morphological, physiological, behavioral and molecular phenotypes.

BACKGROUND: Mahi-mahi (Coryphaena hippurus) is a commercially and ecologically important fish species that is widely distributed in tropical and subtropical waters. Biological attributes and reproductive capacities of mahi-mahi make it a tractable model for experimental studies. In this study, life development of cultured mahi-mahi from the zygote stage to adult has been described. RESULTS: A comprehensive developmental table has been created reporting development as primarily detailed observations of morphology. Additionally, physiological, behavioral, and molecular landmarks have been described to significantly contribute in the understanding of mahi life development. CONCLUSION: Remarkably, despite the vast difference in adult size, many developmental landmarks of mahi map quite closely onto the development and growth of Zebrafish and other warm-water, active Teleost fishes.