ABSTRACT
Immunosensors are promising alternatives as detection platforms for the current gold standards methods. Electrochemical immunosensors have already proven their capability for the sensitive, selective, detection of target biomarkers specific to COVID-19, varying cancers or Alzheimer's disease, etc. Among the electrochemical techniques, electrochemical impedance spectroscopy (EIS) is a highly sensitive technique which examines the impedance of an electrochemical cell over a range of frequencies. There are several important critical requirements for the construction of successful impedimetric immunosensor. The applied surface chemistry and immobilisation protocol have impact on the electroanalytical performance of the developed immunosensors. In this Review, we summarise the building blocks of immunosensors based on EIS, including self-assembly monolayers, nanomaterials, polymers, immobilisation protocols and antibody orientation.
ABSTRACT
Bovine antibodies, such as immunoglobulin G (IgG), cannot pass the placental barrier and as such are not transferred from the mother to the foetus, in utero. Instead a calf must absorb antibodies following ingestion of colostrum postpartum. Failure of Passive Transfer (FPT) is a condition that predisposes calves to development of disease and increases the risk of mortality. Thus, continuous early monitoring of IgG absorption in a calf, within the first 6 to 12 hours of life, is imperative to allow faster treatment and prevent FPT. In this paper, we present the development of a label-free impedimetric immunosensor device for bovine IgG in serum and demonstrate its suitability to determine early FPT in new-born calves. The developed sensors were challenged to discriminate between new born calf sera, both pre- and post-colostrum feeding, and demonstrated efficient detection of IgG in under 15 minutes. Such a device could enable rapid determination of FPT, thereby improving calves' vitality and survival rate.
