ABSTRACT
North American martens are forest dependent, influenced by human activity, and climate vulnerable. They have long been managed and harvested throughout their range as the American marten (Martes americana). Recent work has expanded evidence for the original description of two species in North America - M. americana and the Pacific Coast marten, M. caurina - but the geographic boundary between these groups has not been described in detail. From 2010 to 2016 we deployed 734 multi-taxa winter bait stations across a 53,474 km2 study area spanning seven mountain ranges within the anticipated contact zone along the border of Canada and the United States. We collected marten hair samples and developed genotypes for 15 polymorphic microsatellite loci for 235 individuals, and 493 base-pair sequences of the mtDNA gene COI for 175 of those individuals. Both nuclear and mitochondrial genetic structure identified a sharp break across the Clark Fork Valley, United States with M. americana and M. caurina occurring north and south of the break, respectively. We estimated global effective population size (N e ) for each mountain range, clinal genetic neighborhood sizes (NS), calculated observed (H o ) and expected (H e ) heterozygosity, fixation index (F ST ), and clinal measures of allelic richness (Ar), H o , and inbreeding coefficient (F IS ). Despite substantial genetic structure, we detected hybridization along the fracture zone with both contemporary (nuclear DNA) and historic (mtDNA) gene flow. Marten populations in our study area are highly structured and the break across the fracture zone being the largest documented in North America (F ST range 0.21-0.34, mean = 0.27). With the exception of the Coeur d'Alene Mountains, marten were well distributed across higher elevation portions of our sampling area. Clinal NS values were variable suggesting substantial heterogeneity in marten density and movement. For both M. americana and M. caurina, elevationaly dependent gene flow and high genetic population structure suggest that connectivity corridors will be important to ensuring long-term population persistence. Our study is an example of how a combination of global and clinal molecular data analyses can provide important information for natural resource management.
ABSTRACT
Winter bait stations are becoming a commonly used technique for multispecies inventory and monitoring but a technical evaluation of their effectiveness is lacking. Bait stations have three components: carcass attractant, remote camera, and hair snare. Our 22,975 km2 mountainous study area was stratified with a 5 × 5 km sampling grid centered on northern Idaho and including portions of Washington, Montana, and British Columbia. From 2010-14, we conducted 563 sampling sessions at 497 bait stations in 453 5 × 5 km cells. We evaluated the effectiveness of cameras and hair snare DNA collection at stations to detect species and individual animals, factors affecting DNA viability, the effectiveness of re-visiting stations, and the influence of elevation, seasonality, and latency on species detections. Cameras were more effective at detecting multiple species than DNA hair snaring. Length of deployment time and elevation increased genetic species ID success but individual ID success rates were increased only by collecting hairs earlier in the season. Re-visiting stations did not change camera or genetic species detection results but did increase the number of individual genotypes identified. Marten and fisher were detected quickly while bobcat and coyote showed longer latency to detection. Seasonality significantly affected coyote and bobcat detections but not marten, fisher, or weasel. Multispecies bait station study design should incorporate mixed elevation sites with stratified seasonality. Priority should be given to including cameras as components of bait stations over hair snares, unless there is a specific genetic goal to the study. A hair snare component should be added, however, if individual ID or genetic data are necessary. Winter stations should be deployed a minimum of 45-60 days to allow for detection of low density species and species with long latency to detection times. Hair samples should be collected prior to DNA-degrading late season rain events. Re-visiting stations does not change which species are detected at stations; therefore, studies with objectives to delineate species presence or distribution will be more effective if they focus on deploying more stations across a broader landscape in lieu of surveying the same site multiple times.
ABSTRACT
BACKGROUND: Preliminary research with the Categorization Program (CP) indicated that this therapeutic modality is beneficial in improving cognitive abilities in survivors of moderate-to-severe traumatic brain injury (TBI). This study provides additional evidence for the use of the CP in postacute TBI cognitive rehabilitation. METHODS: Twenty-one participants in the experimental group received the CP training, and 14 participants in the control group received the conventional treatment used at their rehabilitation center. Following neuropsychological testing, participants began their therapy program. RESULTS: There was no significant difference in the baseline performance of the 2 TBI groups on any of the measures. CP-dependent measures correlated significantly with several neuropsychological tests. Both groups improved in their neuropsychological test performance and on functional outcomes tests. However, subjects in the experimental group improved on more tests than participants in the control group. Posttest performance of subjects in the TBI control group was significantly lower on the CP Test 1 and CP Test 2 as compared with the experimental group. Furthermore, the performance of participants in the CP group improved across the 3 probe tasks demonstrating generalizability to new tasks; the performance of participants in the control group did not improve. CONCLUSIONS: The CP is an effective therapy method to reduce categorization impairment and improve cognitive performance of survivors of TBI who are enrolled in postacute rehabilitation. This study contributes to the growing body of evidence supporting cognitive rehabilitation efforts after TBI.
