Drivers of plant diversity, community composition, functional traits, and soil processes along an alpine gradient in the central Chilean Andes.

High alpine regions are threatened but understudied ecosystems that harbor diverse endemic species, making them an important biome for testing the role of environmental factors in driving functional trait-mediated community assembly processes. We tested the hypothesis that plant community assembly along a climatic and elevation gradient is influenced by shifts in habitat suitability, which drive plant functional, phylogenetic, and spectral diversity. In a high mountain system (2400-3500 m) Región Metropolitana in the central Chilean Andes (33°S, 70°W). We surveyed vegetation and spectroscopic reflectance (400-2400 nm) to quantify taxonomic, phylogenetic, functional, and spectral diversity at five sites from 2400 to 3500 m elevation. We characterized soil attributes and processes by measuring water content, carbon and nitrogen, and net nitrogen mineralization rates. At high elevation, colder temperatures reduced available soil nitrogen, while at warmer, lower elevations, soil moisture was lower. Metrics of taxonomic, functional, and spectral alpha diversity peaked at mid-elevations, while phylogenetic species richness was highest at low elevation. Leaf nitrogen increased with elevation at the community level and within individual species, consistent with global patterns of increasing leaf nitrogen with colder temperatures. The increase in leaf nitrogen, coupled with shifts in taxonomic and functional diversity associated with turnover in lineages, indicate that the ability to acquire and retain nitrogen in colder temperatures may be important in plant community assembly in this range. Such environmental filters have important implications for forecasting shifts in alpine plant communities under a warming climate.

Flowering Phenology Adjustment and Flower Longevity in a South American Alpine Species.

Delayed flowering due to later snowmelt and colder temperatures at higher elevations in the alpine are expected to lead to flowering phenological adjustment to prevent decoupling of peak flowering from the warmest time of the year, thereby favoring pollination. However, even if flowering is brought forward in the season at higher elevations, an elevational temperature gap is likely to remain between the high- and low-elevation populations of a species at the time these reach peak flowering on account of the atmospheric reduction in temperature with increasing elevation. The negative effect of this temperature gap on pollination could be compensated by plastically-prolonged flower life spans at higher elevations, increasing the probability of pollination. In a tightly temperature-controlled study, the flowering phenology adjustment and flower longevity compensation hypotheses were investigated in an alpine species in the Andes of central Chile. The snow free period varied from 7 to 8.2 months over 810 m elevation. Temperatures were suitable for growth on 82-98% of the snow free days. Flowering onset was temporally displaced at the rate of 4.6 d per 100 m increase in elevation and flowering was more synchronous at higher elevations. Flowering phenology was adjusted over elevation. The latter was manifest in thermal sums tending to decrease with elevation for population flowering onset, 50% flowering, and peak flowering when the lower thermal limit for growth (TBASE) was held constant over elevation. For TBASE graded over elevation so as to reflect the growing season temperature decline, thermal sums did not vary with elevation, opening the door to a possible elevational decline in the thermal temperature threshold for growth. Potential flower longevity was reduced by passive warming and was more prolonged in natural populations when temperatures were lower, indicating a plastic trait. Pollination rates, as evaluated with the Relative Pollination Rate index (RPR), when weighted for differences in floral abundance over the flowering season, declined with elevation as did fruit set. Contrary to expectation, the life-spans of flowers at higher elevations were not more prolonged and failed to compensate for the elevational decrease in pollination rates. Although strong evidence for phenological adjustment was forthcoming, flower longevity compensation did not occur over Oxalis squamata´s elevational range. Thus, flower longevity compensation is not applicable in all alpine species. Comparison with work conducted several decades ago on the same species in the same area provides valuable clues regarding the effects of climate change on flowering phenology and fitness in the central Chilean alpine where temperatures have been increasing and winter snow accumulation has been declining.

Extreme Drought Affects Visitation and Seed Set in a Plant Species in the Central Chilean Andes Heavily Dependent on Hummingbird Pollination.

Rising temperatures and increasing drought in Mediterranean-type climate areas are expected to affect plant-pollinator interactions, especially in plant species with specialised pollination. Central Chile experienced a mega drought between 2010 and 2020 which reached an extreme in the austral summer of 2019-2020. Based on intensive pollinator sampling and floral studies we show that the subalpine form of Mutisia subulata (Asteraceae) is a specialised hummingbird-pollinated species. In a two-year study which included the severest drought year, we quantified visitation frequency, flower-head density, flower-head visitation rates, two measures of floral longevity, nectar characteristics and seed set and monitored climatic variables to detect direct and indirect climate-related effects on pollinator visitation. Flower-head density, nectar standing crop and seed set were significantly reduced in the severest drought year while nectar concentration increased. The best model to explain visitation frequency included flower-head density, relative humidity, temperature, and nectar standing crop with highly significant effects of the first three variables. Results for flower-head density suggest hummingbirds were able to associate visual signals with reduced resource availability and/or were less abundant. The negative effect of lower relative humidity suggests the birds were able to perceive differences in nectar concentration. Reduced seed set per flower-head together with the availability of far fewer ovules in the 2019-2020 austral summer would have resulted in a major reduction in seed set. Longer and more intense droughts in this century could threaten local population persistence in M. subulata.