ABSTRACT
We studied the antibiotic sensitivity of injured coliforms isolated from drinking water of La Plata, Argentina. The antibiotic sensitivity test by the agar diffusion method were proved in: Klebsiella oxytoca (14 strains), Enterobacter aerogenes (4 strains) and Enterobacter cloacae genomic group 3 (14 strains). We found that while these impaired total coliforms were sensitive to piperacillin-tazobactam (TAZ), netilmicin (NTL), ofloxacin (OFLX), and norfloxacin (NFLX) (100%), they had resistant to aminopenicillin-sulbactam (AMS) and nitrofurantoin (NIT) (100%). The resistance to antibiotics demonstrated in these strains would point to the need to promote a rational and judicious use of antimicrobial agents while at the same time implementing a program of active vigilance aimed at ensuring the highest quality of drinking water throughout the system.
Subject(s)
Anti-Bacterial Agents/pharmacology , Enterobacteriaceae/drug effects , Fresh Water/microbiology , Argentina , Drug Resistance, Bacterial , Enterobacteriaceae/isolation & purification , Microbial Sensitivity Tests , Water Microbiology , Water SupplyABSTRACT
We screened the La Plata drinking water distribution network for fecal and total coliform bacterial indicator by purification procedures, cultivating 66 membrane-filtered samples from the two networks on m-T7 agar. Subterranean and river-derived water yielded 13 and 18 confirmed gram-negative bacillus isolates, with 54% and 72% representing total coliforms, respectively. Those from the former source were Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter aerogenes and from the latter Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter cloacae, genomic group 3. Since 58% of the samples were positive using m-T7 medium it is suggested that the inclusion in standard quality control protocols should be implemented.
Subject(s)
Enterobacteriaceae/isolation & purification , Water Microbiology , Water Pollution/analysis , Water Supply/analysis , Algorithms , Argentina , Bacterial Typing Techniques , Bacteriological Techniques , Culture Media/pharmacology , Enterobacter/classification , Enterobacter/isolation & purification , Enterobacteriaceae/drug effects , Enterobacteriaceae/growth & development , Klebsiella/isolation & purification , Urban Health , Water Purification/methodsABSTRACT
We screened the La Plata drinking water distribution network for fecal and total coliform bacterial indicator by purification procedures, cultivating 66 membrane-filtered samples from the two networks on m-T7 agar. Subterranean and river-derived water yielded 13 and 18 confirmed gram-negative bacillus isolates, with 54 and 72 representing total coliforms, respectively. Those from the former source were Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter aerogenes and from the latter Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter cloacae, genomic group 3. Since 58 of the samples were positive using m-T7 medium it is suggested that the inclusion in standard quality control protocols should be implemented.
Subject(s)
Water Supply/analysis , Enterobacteriaceae , Water Pollution/analysis , Water Microbiology , Algorithms , Argentina , Bacterial Typing Techniques , Bacteriological Techniques , Culture Media , Enterobacter , Enterobacteriaceae , Klebsiella , Water Purification/methods , Urban HealthABSTRACT
We screened the La Plata drinking water distribution network for fecal and total coliform bacterial indicator by purification procedures, cultivating 66 membrane-filtered samples from the two networks on m-T7 agar. Subterranean and river-derived water yielded 13 and 18 confirmed gram-negative bacillus isolates, with 54 and 72 representing total coliforms, respectively. Those from the former source were Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter aerogenes and from the latter Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter cloacae, genomic group 3. Since 58 of the samples were positive using m-T7 medium it is suggested that the inclusion in standard quality control protocols should be implemented.(AU)
Subject(s)
Comparative Study , Enterobacteriaceae/isolation & purification , Water Microbiology , Water Pollution/analysis , Water Supply/analysis , Algorithms , Argentina , Bacterial Typing Techniques , Bacteriological Techniques , Culture Media/pharmacology , Enterobacter/classification , Enterobacter/isolation & purification , Enterobacteriaceae/drug effects , Enterobacteriaceae/growth & development , Klebsiella/isolation & purification , Urban Health , Water Purification/methodsABSTRACT
We screened the La Plata drinking water distribution network for fecal and total coliform bacterial indicator by purification procedures, cultivating 66 membrane-filtered samples from the two networks on m-T7 agar. Subterranean and river-derived water yielded 13 and 18 confirmed gram-negative bacillus isolates, with 54
and 72
representing total coliforms, respectively. Those from the former source were Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter aerogenes and from the latter Klebsiella oxytoca, Enterobacter agglomerans, and Enterobacter cloacae, genomic group 3. Since 58
of the samples were positive using m-T7 medium it is suggested that the inclusion in standard quality control protocols should be implemented.
