ABSTRACT
Nanomaterials have become increasingly important in medicine, manufacturing, and consumer products. A fundamental understanding of the effects of nanoparticles (NPs) and their interactions with biomolecules and organismal systems has yet to be achieved. In this chapter, we firstly provide a brief review of the interactions between nanoparticles and biological systems. We then provide an example by describing a novel method to assess the effects of NPs on biological systems, using insects as a model. Nanoparticles were injected into the central nervous system of the discoid cockroach (Blaberus discoidalis). It was found that insects became hyperactive compared to negative control (water injections). Our method could provide a generic method of assessing nanoparticles toxicity.
Subject(s)
Cockroaches/physiology , Motor Activity/drug effects , Nanoparticles/toxicity , Animals , Central Nervous System/chemistry , Central Nervous System/drug effects , Central Nervous System/physiology , Cockroaches/drug effects , Female , Male , Models, Animal , Nanoparticles/administration & dosageABSTRACT
Nanomaterials have become increasingly important in medicine, manufacturing, and consumer products. The fundamental understanding in effects of nanoparticles (NPs) on and their interactions with biomolecules and organismal systems have yet to be achieved. In this chapter, we firstly provide a brief review of the interactions between nanoparticles and biological systems. We will then provide an example by describing a novel method to assess the effects of NPs on biological systems, using insects as a model. Nanoparticles were injected into the central nervous system of the discoid cockroach (Blaberus discoidalis). It was found that insects became hyperactive compared to negative control (water injections). Our method could provide a generic method of assessing nanoparticles toxicity.
Subject(s)
Cockroaches , Magnetite Nanoparticles/toxicity , Toxicity Tests/methods , Animals , Central Nervous System/drug effects , Female , Magnetite Nanoparticles/administration & dosage , Magnetite Nanoparticles/chemistry , Male , Models, BiologicalABSTRACT
BACKGROUND: Nanoparticles (NPs) are widely studied for biomedical applications. Understanding interactions between NPs and biomolecules or cells has yet to be achieved. Here we present a novel in vivo method to study interactions between NPs and the nervous system of the discoid or false dead-head roach, Blaberus discoidalis. The aims of this study were to present a new and effective method to observe NPs in vivo that opens the door to new methods of study to observe the interactions between NPs and biological systems and to present an inexpensive and easy-to-handle biological system. RESULTS: Negatively charged gold nanoparticles (nAuNPs) of 50 nm in diameter were injected into the central nervous system (CNS) of the insect. By using such a cost effective method, we were able to characterize nAuNPs and to analyze their interactions with a biological system. It showed that the charged particles affected the insect's locomotion. The nAuNPs affected the insect's behavior but had no major impacts on the life expectancy of the cockroach after two months of observation. This was apparently due to the encapsulation of nAuNPs inside the insect's brain. Based on cockroach's daily activity, we believed that the encapsulation occurred in the first 17 days. CONCLUSIONS: The method proposed here is an inexpensive and reliable way of observing the response of biological systems to nanoparticles in-vivo. It opens new windows to further understand how nanoparticles affect neural communication by monitoring insect activity and locomotion.
