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1.
Artif Cells Nanomed Biotechnol ; 50(1): 158-167, 2022 Dec.
Article in English | MEDLINE | ID: mdl-35713365

ABSTRACT

The aim of this study was to evaluate the effect of multilamellar vesicles (MLVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in co-culture with in vitro-produced bovine embryos (IVPEs). The stability of five concentrations of MLVs (1.0, 1.25, 1.5, 1.75, and 2.0 mM) produced using ultrapure water or embryonic culture medium with 24 or 48 h of incubation at 38.5 °C with 5% CO2 was assessed. In addition, the toxicity of MLVs and their modulation of the lipid profile of the plasma membrane of IVPEs were evaluated after 48 h of co-culture. Both media allowed the production of MLVs. Incubation (24 and 48 h) did not impair the MLV structure but affected the average diameter. The rate of blastocyst production was not reduced, demonstrating the nontoxicity of the MLVs even at 2.0 mmol/L. The lipid profile of the embryos was different depending on the MLV concentration. In comparison with control embryos, embryos cultured with MLVs at 2.0 mmol/L had a higher relative abundance of six lipid ions (m/z 720.6, 754.9, 759.0, 779.1, 781.2, and 797.3). This study sheds light on a new culture system in which the MLV concentration could change the lipid profile of the embryonic cell membrane in a dose-dependent manner.


Subject(s)
Blastocyst , Lipid Bilayers , Animals , Cattle , Cell Membrane , Lipid Bilayers/chemistry
2.
Reprod Fertil Dev ; 30(10): 1314-1328, 2018 Oct.
Article in English | MEDLINE | ID: mdl-29681258

ABSTRACT

Mammalian oocytes resume meiosis spontaneously after removal from the ovarian follicle. We tested the effects of a 2-h prematuration treatment (Pre-IVM) with forskolin (FSK) and 3-isobutyl-1-methylxanthine (IBMX) in bovine cumulus-oocyte complexes (COCs) on the lipid content of oocytes and blastocysts, on the membrane lipid composition of blastocysts and on the transcriptional profiling of cumulus cells and blastocysts in a high-throughput platform. Embryonic development rates to the morula (mean 56.1%) or blastocyst (mean 26.3%) stages were unaffected by treatment. Lipid content was not affected after Pre-IVM, but was increased after IVM in treated oocytes. Conversely, the lipid content was reduced in Pre-IVM blastocysts. Pre-IVM COCs generated blastocysts containing blastomeres with more unsaturated lipids in their membranes. Pre-IVM also altered the relative abundance of 31 gene transcripts after 2h and 16 transcripts after 24h in cumulus cells, while seven transcripts were altered in blastocysts. Our results suggest that the Pre-IVM treatment affected the lipid composition and transcriptional profiles of COCs and blastocysts. Therefore, Pre-IVM with FSK and IBMX could be used either to prevent spontaneous meiotic resumption during IVM or to modulate lipid composition in the membrane and cytoplasm of blastocysts, potentially improving bovine embryos.


Subject(s)
Blastocyst/drug effects , Blastocyst/metabolism , Cumulus Cells/drug effects , Cumulus Cells/metabolism , Cyclic AMP/agonists , Cyclic AMP/metabolism , Lipid Metabolism/drug effects , Oocytes/drug effects , Oocytes/metabolism , 1-Methyl-3-isobutylxanthine/pharmacology , Animals , Blastocyst/cytology , Cattle , Colforsin/pharmacology , Cumulus Cells/cytology , Embryonic Development/drug effects , Embryonic Development/genetics , Female , In Vitro Oocyte Maturation Techniques , Oocytes/growth & development , Pregnancy , RNA, Messenger/genetics , RNA, Messenger/metabolism , Transcriptome/drug effects
3.
Anal Bioanal Chem ; 409(29): 6803-6812, 2017 Nov.
Article in English | MEDLINE | ID: mdl-28948318

ABSTRACT

Banana is one of the most popular fruits in the world but has been substantially impaired by Panama disease in the last years. Fusarium oxysporum f. sp. cubense (Foc) is the causal agent and colonizes banana cultivars from many subgroups with different aggressiveness levels, often leading to plant death while compromising new crops in infested areas. This study has evaluated the ability of MALDI-MS protein and lipid fingerprinting to provide intraspecies classification of Foc isolates and to screen biomolecules related to host-pathogen relationship. The MS data, when inspected via partial least square discriminant analysis (PLS-DA), distinguished the isolates by aggressiveness as well as by specific location and host. Although both lipids and proteins show discriminating tendencies, these differences were more clearly perceived via the protein profiles. Considering that Cavendish cultivar is the more resistant option to endure Foc presence in the field, the lipids and proteins related to this subgroup might have an important role in pathogen adaptation. This study reports a new application of MALDI-MS for the analysis of a banana pathogen with intraspecies classification ability. Graphical abstract MALDI-MS classified Foc isolates by aggressiveness level on banana revealing the additional influence of location and host cultivar on the expression of lipids and proteins.


Subject(s)
Fungal Proteins/chemistry , Fusarium/chemistry , Fusarium/classification , Lipids/chemistry , Peptide Mapping , Plant Diseases/microbiology , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
4.
Mol Biosyst ; 13(11): 2401-2406, 2017 Oct 24.
Article in English | MEDLINE | ID: mdl-28960008

ABSTRACT

Particular lipid profiles have been found in two different protozoa of the Leishmania genus. Leishmania infantum, a visceral leishmaniasis causative agent and Leishmania amazonensis, a cutaneous leishmaniasis, reveal distinctive lipid contents of phosphatidylethanolamine and phosphatidylserine plasmalogens, sphingolipids, phosphatidylinositols, phosphatidylcholine, and phosphatidylethanolamine, which have been shown to be related to species, life-cycle of the parasite, and macrophage infection. L. infantum displayed a higher content of phosphatidylethanolamine plasmalogens than L. amazonensis, which may help to differentiate their unique clinical manifestations. Phosphatidylserines plasmalogens are also found to be an important lipid class for the intracellular form of the parasite. Our findings also reveal lipid classes that may be involved in visceralization pathways and parasite differentiation.


Subject(s)
Leishmania infantum , Leishmania , Lipid Metabolism , Macrophages/metabolism , Macrophages/parasitology , Animals , Cells, Cultured , Leishmania/immunology , Leishmania infantum/immunology , Leishmaniasis/parasitology , Lipids , Macrophages/immunology , Macrophages/pathology , Mice
5.
Sci Rep ; 3: 3156, 2013 Nov 06.
Article in English | MEDLINE | ID: mdl-24193576

ABSTRACT

Arthropods produce a great variety of natural compounds, many of which have unexplored biosynthesis. Among the armored harvestmen (Arachnida: Opiliones) of the suborder Laniatores, the defensive gland exudates contain vinyl ketones and other constituents of supposed polyketide origin. We have studied the biosynthesis of 1-hepten-3-one in the Neotropical harvestman Iporangaia pustulosa by feeding individuals with ¹³C-labeled precursors, demonstrating its mixed acetate/propionate origin. ¹³C NMR spectroscopy showed an unusual labeling pattern suggesting different propionate sources for starting and extender units. Our analysis also indicates the presence of methylmalonyl-CoA mutase, converting acetate into propionyl-CoA via succinyl-CoA, together with other C3 unit routes. This is the first biosynthetic study of alkyl vinyl ketones in arthropods. Our results shed light on the origin and diversification of chemical compounds in a major arthropod group.


Subject(s)
Alkenes/metabolism , Arachnida/metabolism , Ketones/metabolism , Acyl Coenzyme A/metabolism , Animals , Arachnida/enzymology , Carbon Isotopes/chemistry , Ketones/chemistry , Magnetic Resonance Spectroscopy , Methylmalonyl-CoA Mutase/metabolism , Propionates/chemistry , Propionates/metabolism
6.
Molecules ; 18(9): 11429-51, 2013 Sep 16.
Article in English | MEDLINE | ID: mdl-24043140

ABSTRACT

Benzoquinones are usually present in arthropod defence exudates. Here, we describe the chemical profiles of 12 harvestman species belonging to the neotropical family Gonyleptidae. Nine of the studied species produced benzoquinones, while three produced alkyl phenols. Two benzoquinones and one phenol exhibited biological activity against bacteria and fungi. We also studied the biosynthesis of 2-ethyl-1,4-benzoquinone by feeding Magnispina neptunus individuals with ¹³C-labelled precursors; the benzoquinones were biosynthesised through a polyketide pathway using acetate and propionate building blocks.


Subject(s)
Arachnida/metabolism , Benzoquinones/chemistry , Phenols/chemistry , Animals , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/metabolism , Anti-Bacterial Agents/pharmacology , Antifungal Agents/chemistry , Antifungal Agents/metabolism , Antifungal Agents/pharmacology , Arachnida/chemistry , Bacillus/drug effects , Benzoquinones/metabolism , Benzoquinones/pharmacology , Biosynthetic Pathways , Candida albicans/drug effects , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Micrococcus luteus/drug effects , Molecular Structure , Phenols/metabolism , Phenols/pharmacology , Pseudomonas aeruginosa/drug effects
7.
J Nat Prod ; 76(9): 1559-64, 2013 Sep 27.
Article in English | MEDLINE | ID: mdl-23978079

ABSTRACT

Four species of gonyleptid harvestmen, Acanthogonyleptes pulcher, Gonyleptes saprophilus (Gonyleptinae), Sodreana barbiellini, and Sodreana leprevosti (Sodreaninae), were examined by GC-MS and ¹³H NMR. All of these species release vinyl ketones, and three of them produce the corresponding pyranyl ketones, which are presumed hetero-Diels-Alder (HDA) dimers. The vinyl ketones 5-methyl-1-hexen-3-one, rac-4-methyl-1-hexen-3-one, and (S)-4-methyl-1-hexen-3-one were synthesized. Natural 4-methyl-1-hexen-3-one is present as a single stereoisomer and has the R-configuration. Vinyl ketone dimers (HDA dimers) were also observed in the scent gland exudate and characterized by HRMS, ¹³C NMR, and ¹H NMR chemical shifts of the pyranyl moiety.


Subject(s)
Arachnida/chemistry , Ketones/chemistry , Pyrans/chemistry , Scent Glands/chemistry , Vinyl Compounds/chemistry , Animals , Arachnida/classification , Arachnida/genetics , Gas Chromatography-Mass Spectrometry , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Scent Glands/metabolism , Stereoisomerism
8.
J Nat Prod ; 74(4): 658-63, 2011 Apr 25.
Article in English | MEDLINE | ID: mdl-21361365

ABSTRACT

The defensive secretions of five neotropical species of harvestmen (Opiliones: Gonyleptidae) from the Brazilian Atlantic Forest were analyzed and chemically characterized by GC-MS and NMR methods. Three of the species, Cobania picea, Roweria virescens, and Serracutisoma proximum, secrete a mixture of 2,3-dimethyl-1,4-benzoquinone and 2-ethyl-3-methyl-1,4-benzoquinone. The secretions produced by the other two species, Iporangaia pustulosa and Neosadocus maximus, contain 1-hepten-3-one, 5-methyl-1-hexen-3-one, and 1-(6-butyl-3,4-dihydro-2H-pyran-2-yl)pentanone (1) as major components, as well as 2,3-dimethyl-1,4-benzoquinone and 2-ethyl-3-methyl-1,4-benzoquinone as minor constituents. The dihydropyran 1-(6-butyl-3,4-dihydro-2H-pyran-2-yl)pentanone (1) is a new natural product, composed of two 1-hepten-3-one subunits formally linked in a hetero-Diels-Alder reaction. The natural product was proven to be racemic, and its biogenetic origin is discussed.


Subject(s)
Arachnida/chemistry , Pyrans/isolation & purification , Animals , Brazil , Gas Chromatography-Mass Spectrometry , Male , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Pentanones , Pyrans/chemistry , Pyrans/pharmacology , Scent Glands/metabolism , Stereoisomerism
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