Tuning the Chemical and Electrochemical Properties of Paper-Based Carbon Electrodes by Pyrolysis of Polydopamine.

Electrochemical paper-based analytical devices represent an important platform for portable, low-cost, affordable, and decentralized diagnostics. For this kind of application, chemical functionalization plays a pivotal role to ensure high clinical performance by tuning surface properties and the area of electrodes. However, controlling different surface properties of electrodes by using a single functionalization route is still challenging. In this work, we attempted to tune the wettability, chemical composition, and electroactive area of carbon-paper-based devices by thermally treating polydopamine (PDA) at different temperatures. PDA films were deposited onto pyrolyzed paper (PP) electrodes and thermally treated in the range of 300-1000 °C. After deposition of PDA, the surface is rich in nitrogen and oxygen, it is superhydrophilic, and it has a high electroactive area. As the temperature increases, the surface becomes hydrophobic, and the electroactive area decreases. The surface modifications were followed by Raman, X-ray photoelectron microscopy (XPS), laser scanning confocal microscopy (LSCM), contact angle, scanning electron microscopy (SEM-EDS), electrical measurements, transmission electron microscopy (TEM), and electrochemical experiments. In addition, the chemical composition of nitrogen species can be tuned on the surface. As a proof of concept, we employed PDA-treated surfaces to anchor [AuCl4]- ions. After electrochemical reduction, we observed that it is possible to control the size of the nanoparticles on the surface. Our route opens a new avenue to add versatility to electrochemical interfaces in the field of paper-based electrochemical biosensors.

Scalable and green formation of graphitic nanolayers produces highly conductive pyrolyzed paper toward sensitive electrochemical sensors.

While pyrolyzed paper (PP) is a green and abundant material that can provide functionalized electrodes with wide detection windows for a plethora of targets, it poses long-standing challenges against sensing assays such as poor electrical conductivity, with resistivities generally higher than 200.0 mΩ cm (e.g., gold and silver show resistivities 1000-fold lower, ∼0.2 mΩ cm). In this regard, the fundamental hypothesis that drives this work is whether a scalable, cost-effective, and eco-friendly strategy is capable of significantly reducing the resistivity of PP electrodes toward the development of sensitive electrochemical sensors, whether faradaic or capacitive. We address this hypothesis by simply annealing PP under an isopropanol atmosphere for 1 h, reaching resistivities as low as 7 mΩ cm. Specifically, the annealing of PP at 800 or 1000 °C under isopropanol vapor leads to the formation of a highly graphitic nanolayer (∼15 nm) on the PP surface, boosting conductivity as the delocalization of π electrons stemming from carbon sp2 is favored. The reduction of carbonyl groups and the deposition of dehydrated isopropanol during the annealing process are hypothesized herein as the dominant PP graphitization mechanisms. Electrochemical analyses demonstrated the capability of the annealed PP to increase the charge-transfer kinetics, with the optimum heterogeneous standard rate constant being roughly 3.6 × 10-3 cm s-1. This value is larger than the constants reported for other carbon electrodes and indium tin oxide. Furthermore, freestanding fingers of the annealed PP were prototyped using a knife plotter to fabricate impedimetric on-leaf electrodes. These wearable sensors ensured the real-time and in situ monitoring of the loss of water content from soy leaves, outperforming non-annealed electrodes in terms of reproducibility and sensitivity. Such an application is of pivotal importance for precision agriculture and development of agricultural inputs. This work addresses the foundations for the achievement of conductive PP in a scalable, low-cost, simple, and eco-friendly way, i.e. without producing any liquid chemical waste, providing new opportunities to translate PP-based sensitive electrochemical devices into practical use.

Recent progress and future perspectives of polydopamine nanofilms toward functional electrochemical sensors.

Since its discovery in 2007, polydopamine nanofilms have been widely used in many areas for surface functionalization. The simple and low-cost preparation method of the nanofilms with tunable thickness can incorporate amine and oxygen-rich chemical groups in virtually any interface. The remarkable advantages of this route have been successfully used in the field of electrochemical sensors. The self-adhesive properties of polydopamine are used to attach nanomaterials onto the electrode's surface and add chemical groups that can be explored to immobilize recognizing species for the development of biosensors. Thus, the combination of 2D materials, nanoparticles, and other materials with polydopamine has been successfully demonstrated to improve the selectivity and sensitivity of electrochemical sensors. In this review, we highlight some interesting properties of polydopamine and some applications where polydopamine plays an important role in the field of electrochemical sensors.

Biocompatible Wearable Electrodes on Leaves toward the On-Site Monitoring of Water Loss from Plants.

Impedimetric wearable sensors are a promising strategy for determining the loss of water content (LWC) from leaves because they can afford on-site and nondestructive quantification of cellular water from a single measurement. Because the water content is a key marker of leaf health, monitoring of the LWC can lend key insights into daily practice in precision agriculture, toxicity studies, and the development of agricultural inputs. Ongoing challenges with this monitoring are the on-leaf adhesion, compatibility, scalability, and reproducibility of the electrodes, especially when subjected to long-term measurements. This paper introduces a set of sensing material, technological, and data processing solutions that overwhelm such obstacles. Mass-production-suitable electrodes consisting of stand-alone Ni films obtained by well-established microfabrication methods or ecofriendly pyrolyzed paper enabled reproducible determination of the LWC from soy leaves with optimized sensibilities of 27.0 (Ni) and 17.5 kΩ %-1 (paper). The freestanding design of the Ni electrodes was further key to delivering high on-leaf adhesion and long-term compatibility. Their impedances remained unchanged under the action of wind at velocities of up to 2.00 m s-1, whereas X-ray nanoprobe fluorescence assays allowed us to confirm the Ni sensor compatibility by the monitoring of the soy leaf health in an electrode-exposed area. Both electrodes operated through direct transfer of the conductive materials on hairy soy leaves using an ordinary adhesive tape. We used a hand-held and low-power potentiostat with wireless connection to a smartphone to determine the LWC over 24 h. Impressively, a machine-learning model was able to convert the sensing responses into a simple mathematical equation that gauged the impairments on the water content at two temperatures (30 and 20 °C) with reduced root-mean-square errors (0.1% up to 0.3%). These data suggest broad applicability of the platform by enabling direct determination of the LWC from leaves even at variable temperatures. Overall, our findings may help to pave the way for translating "sense-act" technologies into practice toward the on-site and remote investigation of plant drought stress. These platforms can provide key information for aiding efficient data-driven management and guiding decision-making steps.

Graphene oxide fibers by microfluidics assembly: a strategy for structural and dimensional control.

Graphene oxide (GO) microfibers with controlled and homogeneous shapes and tunable diameters were fabricated using the 3 dimensional (3D) hydrodynamic focusing concept on a microfluidic device. Thermal and microwave treatments are used to obtain reduced graphene oxide (rGO) microfibers with outstanding electrical properties, thus enabling the development of ionic liquid-gate field-effect transistors (FET) based on graphene derivative microfibers.

Treinamento de manipuladores de alimentos: merendeiras / Training of food manipulators: snack makers

Um dos fatores determinantes da saúde é a alimentação, que depende da qualidade sanitária dos alimentos. No ambiente escolar, a prática de higiene deve ser empregada em todas as etapas do preparo de refeições. Com o objetivo de capacitar os manipuladores de alimentos, em escolas, proporcionando-lhes qualificação, foi realizado um treinamento das merendeiras da rede municipal de ensino de Guiricema-MG, em fevereiro de 2003, perfazendo um total de 8 horas. Foram abordados aspectos relacionados à prática de higiene na produção de alimentos, tendo como metodologia dinâmica a aplicação de questionários e a utilização de cartazes ilustrados, que serviram como técnicas de instrução para facilitar e favorecer uma melhor integração entre os membros do grupo e os instrutores. Conseguiu-se uma participação satisfatória de 100 por cento das merendeiras, que mostraram-se interessadas e atentas, procurando a todo o momento esclarecer as dúvidas existentes, bem como relatar experiências vivenciadas. Com relação à dinâmica realizada, todas foram unânimes na escolha da candidata, que apresentava características adequadas para o desempenho de sua função no ambiente escolar. Observou-se que 92,3 por cento das participantes acertaram os questionamentos iniciais, havendo dúvidas em relação a patogenicidade dos microrganismos e à correta utilização dos panos de prato. Constatou-se que as merendeiras detinham a maior parte do conhecimento, porém apresentavam resistência em segui-lo. A realização de treinamentos periódicos, visando a correta adoção e manutenção das boas práticas no ambiente de trabalho, torna-se necessária, como forma de reciclagem constante para que as mesmas possam desempenhar suas funções no ambiente escolar e domiciliar.