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J Clin Microbiol ; 54(7): 1896-1898, 2016 07.
Article in English | MEDLINE | ID: mdl-27076665

ABSTRACT

In this study, we evaluated the influence of distinct bacterial growth media on detection of carbapenemase hydrolysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. False-negative results were observed for OXA-25-, OXA-26-, and OXA-72-producing Acinetobacter baumannii isolates grown on MacConkey agar medium. The other culture media showed 100% sensitivity and 100% specificity for detecting carbapenemase.


Subject(s)
Acinetobacter baumannii/enzymology , Bacterial Proteins/analysis , Carbapenems/metabolism , Culture Media/chemistry , Hydrolysis , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , beta-Lactamases/analysis , False Negative Reactions , Sensitivity and Specificity
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