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Comparison of test specificities of commercial antigen-based assays and in-house PCR methods for detection of rotavirus in stool specimens.

Ye, S; Lambert, S B; Grimwood, K; Roczo-Farkas, S; Nimmo, G R; Sloots, T P; Kirkwood, C D; Whiley, D M.

J Clin Microbiol ; 53(1): 295-7, 2015 Jan.

Article in English | MEDLINE | ID: mdl-25339400

ABSTRACT

Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection.

Subject(s)

Chromatography, Affinity/methods , Feces/virology , Polymerase Chain Reaction/methods , Rotavirus Infections/diagnosis , Rotavirus Infections/virology , Rotavirus/genetics , Rotavirus/isolation & purification , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Middle Aged , Reproducibility of Results , Sensitivity and Specificity , Young Adult

Evidence of false-positive results in a commercially available rotavirus assay in the vaccine era, Australia, 2011 to 2012.

Ye, S; Roczo-Farkas, S; Whiley, Dm; Lambert, Sb; Robson, J; Heney, C; Nimmo, G; Grimwood, K; Sloots, Tp; Kirkwood, Cd.

Euro Surveill ; 18(21)2013 May 23.

Article in English | MEDLINE | ID: mdl-23725979

Subject(s)

Chromatography, Affinity/standards , Product Surveillance, Postmarketing , Rotavirus Infections/diagnosis , Australia , False Positive Reactions , Humans , Rotavirus Vaccines , Sensitivity and Specificity

ABSTRACT

Subject(s)

Subject(s)

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