ABSTRACT
BACKGROUND: Thyroid gland is highly dependent on dietary intake of iodine for normal function, so it is particularly subjected to "endocrine disruptor" action. The human sodium/iodide symporter (hNIS) is an integral plasma membrane glycoprotein mediating the active transport of iodide into thyroid follicular cells, a crucial step for thyroid hormone biosynthesis. Beyond to perchlorate and thyocianate ions a few other inhibitors of iodide uptake have been described. AIM: The aim of this study was to investigate if 10 substances usually used as drugs in clinical practice were able to inhibit NIS-mediated iodide uptake in vitro. MATERIALS AND METHODS: A CHO cell line stably expressing hNIS was used to test any inhibition of NIS-mediated iodide uptake exerted by drugs. Perchlorate and thyocianate ions were used as positive controls. RESULTS: None of the analyzed substances was able to significantly inhibit iodide uptake in our system. As we expected, perchlorate and thyocianate ions were able to inhibit iodide uptake in a dose-dependent manner. CONCLUSIONS: In conclusion, we carried out an in vitro assay to evaluate the potential inhibitory effect of common drugs on NISmediated iodide uptake by using CHO-hNIS cells. None of the analyzed substances was able to inhibit iodide uptake; only perchlorate and thyocianate were able to inhibit iodide uptake in a dose-dependent manner.
Subject(s)
CHO Cells/metabolism , Iodides/metabolism , Symporters/metabolism , Thyroid Gland/metabolism , 14-alpha Demethylase Inhibitors/chemistry , 14-alpha Demethylase Inhibitors/pharmacology , Amphotericin B/chemistry , Amphotericin B/pharmacology , Animals , Anti-Allergic Agents/chemistry , Anti-Allergic Agents/pharmacology , Anti-Infective Agents/chemistry , Anti-Infective Agents/pharmacology , Anti-Inflammatory Agents/chemistry , Anti-Inflammatory Agents/pharmacology , Atropine/chemistry , Atropine/pharmacology , Biotin/chemistry , Biotin/pharmacology , Buspirone/chemistry , Buspirone/pharmacology , CHO Cells/drug effects , Cricetinae , Cricetulus , Econazole/chemistry , Econazole/pharmacology , Humans , Hydrocortisone/chemistry , Hydrocortisone/pharmacology , Metronidazole/chemistry , Metronidazole/pharmacology , Muscarinic Antagonists/chemistry , Muscarinic Antagonists/pharmacology , Papaverine/chemistry , Papaverine/pharmacology , Perchlorates/pharmacology , Phosphodiesterase Inhibitors/chemistry , Phosphodiesterase Inhibitors/pharmacology , Promethazine/chemistry , Promethazine/pharmacology , Serotonin Receptor Agonists/chemistry , Serotonin Receptor Agonists/pharmacology , Sulfadiazine/chemistry , Sulfadiazine/pharmacology , Symporters/genetics , Thiocyanates/pharmacology , Thyroid Gland/drug effectsABSTRACT
Se revisaron 101 pacientes diagnosticados de fractura de la extremidad proximal del fémur, ocurridas entre los años 1986-1996. Se escogieron aleatoriamente, a partir de un listado de todos 108 Pacientes intervenidos mediante osteosíntesis con clavos de Ender (748), aquellos pacientes que llevasen un postoperatorio mayor de un año y sus historias clínicas fueron solicitadas del archivo central del hospital sin ninguna instrucción al personal administrativo. Se tuvo en cuenta: edad, sexo, lado, estado previo del paciente (autonomía) en la valoración de los resultados. Todos los pacientes recuperaron su estado previo a la fractura al final del periodo de observación, salvo cuatro casos de exitus en el postoperatorio inmediato por complicaciones de su patología previa. (AU)
