Effects of Submerged Macrophytes on Demography and Filtration Rates of Daphnia and Simocephalus (Crustacea: Cladocera).

Macrophytes and cladocerans represent the main antagonistic groups that regulate phytoplankton biomass; however, the mechanism behind this interaction is unclear. In laboratory conditions, we separately evaluated the effects of three submerged macrophytes (Ceratophyllum demersum, Myriophyllum aquaticum, and Stuckenia pectinata), as well as their exudates, and plant-associated microbiota (POM < 25 µm) + exudates on the population growth of Daphnia cf. pulex and Simocephalus cf. mixtus. Living Ceratophyllum, exudates, and POM < 25 µm + exudates exhibited the most robust positive effects on Simocephalus density and the rate of population increase (r). Subsequently, we examined the effects of Ceratophyllum on the filtration and feeding rates of Simocephalus and Daphnia, revealing significant (p < 0.001) promotion of filtration and feeding in Simocephalus but not in Daphnia. To elucidate the specific effects of this macrophyte on Simocephalus demography, we assessed selected life table variables across the same treatments. The treatments involving exudates and living Ceratophyllum resulted in approximately 40% longer survivorship and significantly (p < 0.01) enhanced fecundity. Our findings indicate that exudates from submerged macrophytes positively influence Simocephalus demography by increasing filtration rates, survivorship, and fecundity. This synergy suggests a substantial impact on phytoplankton abundance.

Screening of Natural Products Inhibitors of SARS-CoV-2 Entry.

The COVID-19 pandemic has led to the search for new molecules with antiviral activity against SARS-CoV-2. The entry of the virus into the cell is one of the main targets for inhibiting SARS-CoV-2 infection. Natural products are an important source of new therapeutic alternatives against diseases. Pseudotyped viruses allow the study of SARS-CoV-2 viral entry inhibitors, and due to their simplicity, they allow the screening of a large number of antiviral candidates in Biosafety Level 2 facilities. We used pseudotyped HIV-1 with the D614G SARS-CoV-2 spike glycoprotein to test its ability to infect ACE2-expressing HEK 293T cells in the presence of diverse natural products, including 21 plant extracts, 7 essential oils, and 13 compounds from plants and fungi. The 50% cytotoxic concentration (CC50) was evaluated using the resazurin method. From these analyses, we determined the inhibitory activity of the extract of Stachytarpheta cayennensis, which had a half-maximal inhibitory concentration (IC50) of 91.65 µg/mL, a CC50 of 693.5 µg/mL, and a selectivity index (SI) of 7.57, indicating its potential use as an inhibitor of SARS-CoV-2 entry. Moreover, our work indicates the usefulness of the pseudotyped-virus system in the screening of SARS-CoV-2 entry inhibitors.

The right ventricle of transplanted hearts at 2,640 meters above sea level. A latin-american experience. / El ventrículo derecho de los corazones trasplantados a 2,640 metros sobre el nivel del mar. Una experiencia latinoamericana

BACKGROUND: High-altitude cardiovascular adaptations increase lung pressure. This effect on the right ventricle (RV) of transplanted hearts at altitudes above 2,500 meters above sea level (masl) has not been described. OBJECTIVE: The objective of the study was to describe echocardiography RV behavior in the immediate post-operative period (Days 1-7 post-Heart transplant [HTx]), 3, 6, 12, and 24 months after HTx in patients at 2640 masl. METHODS: Historical cohort of HTx patients in the period between 2005 and 2019, in a hospital located in Bogotá, Colombia. Socio-demographic, clinical, and echocardiographic evaluation data of the RV at 5 follow-up moments were analyzed. RESULTS: 91 patients underwent HTx, 64% remained at a height > 2500 masl in the post-operative period. Transthoracic echo was available in 37 patients (40.6%). Right ventricular dysfunction was found in 95% of patients, which was predominantly moderate (43%), with improvement 3 months after transplant. The immediate post-operative Tricuspid annular plane systolic excursion was 8.9 ± 4.9 mm, with recovery from the 3rd post-operative month (15.1 ± 3.6 mm) and without significant changes in month 24 (15.8 ± 4.9 mm). Immediate post-operative systolic pulmonary artery pressure (sPAP) was 39.2 ± 8.2 mmHg, showing a decrease at 24 post-operative months (31.0 ± 5.0 mmHg). The 5-year survival was 78% Confidence Interval 95% 60-85. CONCLUSION: After HTx, most patients present right ventricular dysfunction, improving at the 3rd month of transplant. There were no significant differences between patients living at more than 2500 masl and < 2500 masl.

ANTECEDENTES: Las adaptaciones cardiovasculares en la altitud aumentan la presión pulmonar; el efecto de estos cambios sobre el ventrículo derecho de corazones trasplantados a altitudes superiores a 2,500 msnm no ha sido descrito. OBJETIVO: Describir el comportamiento por ecocardiografía transtorácica del ventrículo derecho en el postoperatorio inmediato (días 1­7 post-HTx), 3, 6, 12 y 24 meses después del trasplante cardíaco en pacientes intervenidos a 2,640 ms nm. MÉTODOS: Cohorte histórica de pacientes trasplantados de corazón en un hospital de Bogotá, Colombia, entre 2005 y 2019. Los datos sociodemográficos, clínicos y ecocardiográficos del ventrículo derecho fueron analizados en 5 momentos del seguimiento postoperatorio. RESULTADOS: 91 pacientes fueron sometidos a trasplante cardíaco, el 64% residía a más de 2,500 msnm en el postoperatorio. El ecocardiograma transtorácico estuvo disponibles en 37 pacientes (40.6%). En el 95% de los pacientes se documentó disfunción del ventrículo derecho la cual fue predominantemente moderada (43%), con mejoría al 3 mes del trasplante. El TAPSE en el postoperatorio inmediato fue de 8.9±4.9 mm, con recuperación a partir del tercer mes postoperatorio (15.1±3.6 mm); la mejoría se mantuvo hasta el mes 24 (15.8±4.9 mm). La PsAP postoperatoria inmediata fue de 39.2±8.2 mmHg y disminuyó a los 24 meses (31.0±5.0 mmHg). La supervivencia a los 5 años fue del 78% IC95% 60-85. CONCLUSIONES: Posterior al trasplante cardíaco, la mayoría de los pacientes presentó disfunción ventricular derecha, mejorando al tercer mes del trasplante. No hubo diferencias significativas entre los pacientes que vivían a mas de 2,500 msnm y menos de 2,500 msnm.

Queratoconjuntivitis por fenilefrina confirmada con pruebas alérgicas tras una exploración ordinaria / Phenylephrine keratoconjunctivitis confirmed by allergy testing after routine examination

Las reacciones alérgicas tras el uso de colirios oftálmicos son comunes y pueden manifestarse con queratoconjuntivitis y dermatitis periorbitaria. La introducción reciente de nuevos fármacos tópicos facilita la sospecha clínica, pero en ocasiones se trata de un uso simultáneo de varios medicamentos y la identificación del alérgeno específico se convierte en un reto. Debido a ello, en muchas ocasiones el diagnóstico es de exclusión, ya que no se llega a confirmar con estudio alergológico el agente desencadenante de la reacción. Tal es el caso de la alergia ocular a fenilefrina, dado que son pocos los cuadros clínicos descritos en la literatura donde hay una clara causa inmunológica. Presentamos un caso poco frecuente, una forma grave de queratoconjuntivitis alérgica tras un examen oftalmológico ordinario confirmada tras estudio alergológico completo

Allergic reactions after using ophthalmic eye drops are very common and frequently manifested by ketaconjuntivitis and periorbital dermatitis. Previous introduction of new topical drugs makes the clinical suspicion easier but sometimes the patient uses multiple medications simultaneously and the specific allergen identification become a challenge. That is the reason why in many occasions the diagnosis is of exclusion since the agent triggering the reaction is not confirmed with an allergologic study. Such is the case of ocular allergy to phenylephrine: there are few clinical cases where there is a clear immunologic cause. We report an uncommon case of a patient who develops a severe form of allergic keratoconjuntivitis after routine ophthalmological examination confirmed by complete allergologic study

Proximal retroflexion versus second forward view of the right colon during screening colonoscopy: A multicentre randomized controlled trial.

BACKGROUND: Colonoscopy is the gold standard investigation for the detection of colorectal cancer, but the right colon is more difficult to examine than the left colon. A second examination of the proximal colon has the potential to reduce rates of missed pathology. OBJECTIVE: To determine whether proximal retroflexion improves the adenoma detection rate or other outcomes in the right colon compared with the forward view. METHODS: We performed a multicentre randomized controlled trial of patients from the colorectal cancer screening programme with a positive faecal immunochemical test. Patients were randomized to a second right colon examination using proximal retroflexion or forward view. RESULTS: A total of 692 patients were included. A second examination of the right colon, with an average additional procedure time of 1.62 min, increased the adenoma detection rate by 11%, regardless of the method used (9% proximal retroflexion vs. 12% second forward view, p = 0.21). The adenoma miss rate was 19% (17% proximal retroflexion vs. 20% forward view, p = 0.28) The success rate of retroflexion was 83%, without secondary complications. In the 15.6% of patients in whom lesions were detected during the second pass, endoscopic follow-up was modified by reducing the time of the next colonoscopy. CONCLUSIONS: A second examination of the right colon, either from retroflexion or second forward view, can increase adenoma detection rate and shorten surveillance intervals in patients undergoing screening colonoscopy. This should be emphasized during colonoscopy training and integrated into diagnostic colonoscopy practice.

Cuando tomarse un gin-tonic se convierte en una mala experiencia: exantema fijo medicamentoso por quinina / When a Gin Tonic Can Mean Trouble: Fixed Drug Eruption Due to Quinine

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Reflexiones en una consulta de Atención Primaria en tiempos de pandemia / Reflections in a Primary Care consultation in pandemic times

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Prevalencia del síndrome metabólico en niños de Comodoro Rivadavia / Prevalence of metabolic syndrome in children of Comodoro Rivadavia

INTRODUCCIÓN: la salud es esencial para el crecimiento social. En los últimos años, el énfasis está focalizado en la prevención primaria. En este contexto, el síndrome metabólico (SM) no es ajeno a esta situación, si se tienen en cuenta las consecuencias cardiovasculares, circulatorias o metabólicas que se pueden originar. La infancia constituye el momento oportuno para poder intervenir, ya que se pueden establecer pautas y medidas correctivas. OBJETIVO: el objetivo del presente trabajo fue obtener la prevalencia de SM en niños de Comodoro Rivadavia (Argentina), de tal manera que se pueda aportar información que sirva para establecer estrategias preventivas. MATERIALES Y MÉTODOS: se estudiaron 317 niños (174 mujeres y 143 varones), de edades entre 6 y 11 años, que concurrían habitualmente a centros barriales y a tres escuelas de Comodoro Rivadavia. Con consentimiento informado de los padres, fueron pesados y medidos, se recabaron datos de tensión arterial y circunferencia de cintura y se les extrajo una muestra de sangre para evaluar, triglicéridos, colesterol-HDL y glucemia. RESULTADOS: teniendo en cuenta los criterios del Adult Treatment Panel III (ATP III) modificados por Cook, la prevalencia de SM en la muestra estudiada fue del 3,50% para los varones y del 3,40% para las mujeres, no encontrándose una diferencia estadísticamente significativa de acuerdo con el sexo. Se encontraron valores de circunferencia de cintura aumentada, hipertrigliceridemia e hipertensión arterial y valores disminuidos de HDL. CONCLUSIONES: estos resultados preliminares indican la necesidad de profundizar los estudios para detectar precozmente este síndrome en la edad pediátrica y así evitar el desarrollo de enfermedades crónicas en el futuro

INTRODUCTION: health is essential for social growth. In recent years, there has been an emphasis on primary prevention. In this context, metabolic syndrome (MS) is a relevant issue, given its potential impact on cardiovascular, circulatory and metabolic health. Childhood is the most appropriate time for intervention, as corrective measures and strategies can be implemented in this period. OBJECTIVE: the aim of our study was to establish the prevalence of MS in the children of Comodoro Rivadavia (Argentina) to contribute information that may be useful in the development of preventive strategies. MATERIAL AND METHODS: the study included 317 children (174 girls and 143 boys) aged 6 to 11 years that regularly attended neighbourhood centres and the 3 schools of Comodoro Rivadavia. Having obtained parental informed consent, we measured their weight, height, waist circumference collected and blood pressure and obtained a blood sample to measure serum levels of triglycerides, HDL cholesterol and glucose. RESULTS: applying the Adult Treatment Panel III (ATP III) criteria modified by Cook, we found a prevalence of MS of 3.50% in boys and 3.40% in girls, without a statistically significant difference between the sexes. We found waist circumference values above normal, elevation of triglycerides in blood, raised blood pressure and low levels of HDL cholesterol. CONCLUSIONS: these preliminary results highlight the need of performing more thorough investigations for the early detection of MB in the paediatric age group with the aim of preventing the development of chronic diseases in the future

Implementation of a Tool for the Assessment of the Frail Elderly in a Robotic Agent.

The concern for aging, chronic illness, and dependence is relevant in today's society. The Nursing discipline is responsible for its approach to care. Furthermore, this profession must develop technologies to assist. The main objective is the development of a robotic system for assessment of fall risk and adherence to pharmacological treatment. The magnitude of this prototype design and its validation by experts warrant the attention to support care for the vulnerable and fragile with technology.

Alteraciones retinocoroideas y cáncer pulmonar: primer reporte en población mexicana / Retinochoroidal findings and lung cancer: first report in mexican population

Introducción: El cáncer pulmonar (CP) es el tumor con mayor frecuencia y mortalidad a nivel mundial. Casos de metástasis coroideas y retinopatía asociada a cáncer han sido publicados en CP, sin embargo no existen estudios en población mexicana que describan las posibles alteraciones retinocoroideas y su relación con el estadio de CP. Objetivo: Evaluar a pacientes con CP para determinar la presencia de alteraciones en el segmento posterior y su relación con el estadio del mismo. Materiales y métodos: Estudio transversal y descriptivo de 50 pacientes (100 ojos) con CP. Datos demográficos: edad, sexo, tipo histológico, tiempo de evolución, estadio, tratamiento y comorbilidades. Variables de medición: agudeza visual (LogMAR), biomicroscopía del segmento anterior, registro fotográfico de retina, fluorangiografía retiniana, tomografía de coherencia óptica y electrorretinograma. Cada paciente fue evaluado por dos oftalmólogos. Resultados: Un total de 26 hombres y 24 mujeres fueron evaluados, el promedio de edad fue de 65 años, el tiempo medio del diagnóstico de CP fue de 6 meses siendo el adenocarcinoma el principal tipo histológico (70%), al momento de la evaluación 50% presentaban estadio II y 30% estadio IV. Las alteraciones del segmento posterior encontradas fueron: metástasis coroideas (16%), metástasis retinianas (10%), retinopatía asociada a cáncer (6%) y oclusiones vasculares (4%). La mayoría de los pacientes con alteraciones retinocoroideas se encontraban en estadio IV. Conclusiones: En el CP pueden encontrarse oclusiones vasculares, retinopatía asociada a cáncer y metástasis a coroides y retina con una incidencia mayor a la publicada en la literatura, siendo más frecuentes en estadios avanzados de la enfermedad aunque pueden encontrarse desde el estadio II en pacientes asintomáticos

Introduction: Lung cancer (LC) is the most common tumour, and the leading cause of cancer-related death worldwide. Although cases of choroidal metastasis and cancer-associated retinopathy have been reported in LC, no studies have been conducted on the Mexican population to describe retinochoroidal findings during the course of LC, and the relationship with its stage. Objective: To evaluate patients with a diagnosis of LC, and to describe the posterior segment findings in relationship to the stage of LC. Materials and methods: A cross-sectional and descriptive study was conducted on 50 patients with LC (100 eyes). The demographic data included age, gender, histological type, evolution time, stage, treatment, and comorbidities. The measurement variables included visual acuity (LogMAR), anterior segment biomicroscopy, retinal photography, fluorescein retinal angiography, optical coherence tomography, and electroretinogram. All patients were evaluated by two ophthalmologists. Results: The study included a total of 26 men and 24 women, with a mean age of 65 years, and a mean time from LC diagnosis of 6 months. The principal histological type was adenocarcinoma (70%), and most (50%) were in stage II at the time of evaluation, with 15 (30%) patients having a metastasis (stage IV). The changes in the posterior segment included choroidal metastasis (16%), retinal metastasis (10%), cancer-associated retinopathy (6%), and vascular occlusions (4%). The majority of patients who presented with posterior segment alterations were in stage IV. Conclusions: Vascular occlusions, cancer-associated retinopathy, choroidal and retinal metastases may be found in LC, with an incidence higher than that reported in the literature, especially in advanced stages of LC, although they can be found from stage II in asymptomatic patients

Glaucoma agudo de ángulo cerrado inducido por topiramato con miopización aguda y estrías maculares: a propósito de un caso / Acute narrow-angle glaucoma induced by topiramate with acute myopia and macular striae: A case report

Presentamos el caso clínico de una mujer epiléptica de 29 años en tratamiento con topiramato 25 mg/día desde 9 días previos a la presentación del cuadro, que acude a urgencias por disminución de la agudeza visual (AV) tras incrementar la dosis a 50mg/día 2 días antes. En la exploración presenta un glaucoma agudo de ángulo cerrado (GAAC) bilateral y estrías maculares en ambos ojos (AO) objetivadas mediante retinografía y tomografía de coherencia óptica (OCT). El GAAC es un efecto secundario conocido y ampliamente descrito del topiramato, sin embargo, las estrías maculares que ocasionalmente acompañan al GAAC, aunque se han descrito anteriormente en otros casos, muy pocos las documentan mediante imágenes de retinografía y OCT. Es importante diferenciar un caso de GAAC inducido por topiramato de un caso de GAAC primario ya que difieren en su presentación clínica, mecanismo de acción y tratamiento. Su manejo inadecuado puede tener consecuencias potencialmente graves

We report the case of a 29-year-old epileptic woman who had been on treatment with topiramate 25 mg/day for 9 days. She was referred to the Emergency Department due to reduction in far visual acuity (VA) after increasing the dose to 50mg/day two days before. The ocular examination showed bilateral acute angle closure glaucoma (AACG) and macular striae in both eyes (AO) observed by Retinography and Optical Coherence Tomography (OCT). The AACG is a well-known side effect of topiramate, but the macular striae rarely accompanies it. Although macular striae have been previously described in other cases, very few document those using retinography and OCT images. Therefore, it is important to differentiate a case of AACG induced by topiramate from a case of primary AACG, since they differ in their clinical presentation, mechanism of action, and treatment. Mismanagement can have potentially serious consequences

Oclusión mixta de arteria y vena central de la retina; primera manifestación de lupus en paciente pediátrico / Combined central retinal artery and vein occlusion; first manifestation of lupus in a pediatric patient

La oclusión combinada de arteria y vena central de la retina es poco común en adultos y aún más rara en pacientes jóvenes, siendo las vasculitis y los trastornos tromboembólicos las principales etiologías identificadas. La mayoría de los pacientes tiene un pronóstico sombrío debido al inevitable desarrollo de pérdida visual irreversible y glaucoma neovascular (GNV). Presentamos el caso de un paciente de 14 años de edad que acudió aquejando pérdida visual súbita e indolora en el ojo izquierdo. La mejor agudeza visual corregida fue de percepción de luz, mostrando una exploración oftalmológica y fluorangiográfica característica de oclusión mixta de arteria y vena central de la retina del ojo izquierdo. Las alteraciones encontradas en la exploración física y en los estudios de laboratorio permitieron el diagnóstico de lupus eritematoso sistémico (LES) y síndrome antifosfolípidos. El paciente recibió tratamiento con esteroides y anticoagulante por vía oral, presentando hemorragia vítrea 7 días después debido a tiempos de coagulación prolongados. Para prevenir la aparición de GNV, se inició tratamiento con ranibizumab intravítreo y posteriormente se realizó vitrectomía y endofotocoagulación retiniana. De acuerdo con nuestro conocimiento, este es el primer caso de un paciente masculino pediátrico con LES y oclusión mixta publicado en la literatura, y a su vez el primer caso tratado con antiangiogénicos que no ha desarrollado GNV a 12 meses de seguimiento

Combined central retinal artery and vein occlusion is uncommon in adults and even more so in young people. The main origins are vasculitis and thromboembolic disorders. The prognosis is poor due to irreversible visual loss and the development of neovascular glaucoma (NVG). A 14 year-old male arrived at the clinic complaining of sudden and painless visual loss in the left eye. Best corrected visual acuity was light perception with clinical and fluoro-angiographic findings characteristic of combined central retinal artery and vein occlusion in his left eye. The findings in the systemic and laboratory studies led to a diagnosis of systemic lupus erythematosus (SLE) and antiphospholipid syndrome. The patient received treatment with steroids and oral anticoagulant. Seven days later, due to prolonged coagulation time, he presented with a vitreous haemorrhage. He was then treated with intravitreal ranibizumab in order to prevent NVG. Afterwards, vitrectomy and retinal endophotocoagulation were performed. To the best of our knowledge, this is the first presentation of a male, paediatric with SLE and combined occlusion to be published in the literature, and is also the first case treated with antiangiogenic agents that has not developed NVG at 12 months of follow-up

Quality control project of NGS HLA genotyping for the 17th International HLA and Immunogenetics Workshop.

The 17th International HLA and Immunogenetics Workshop (IHIW) organizers conducted a Pilot Study (PS) in which 13 laboratories (15 groups) participated to assess the performance of the various sequencing library preparation protocols, NGS platforms and software in use prior to the workshop. The organizers sent 50 cell lines to each of the 15 groups, scored the 15 independently generated sets of NGS HLA genotyping data, and generated "consensus" HLA genotypes for each of the 50 cell lines. Proficiency Testing (PT) was subsequently organized using four sets of 24 cell lines, selected from 48 of 50 PS cell lines, to validate the quality of NGS HLA typing data from the 34 participating IHIW laboratories. Completion of the PT program with a minimum score of 95% concordance at the HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1 loci satisfied the requirements to submit NGS HLA typing data for the 17th IHIW projects. Together, these PS and PT efforts constituted the 17th IHIW Quality Control project. Overall PT concordance rates for HLA-A, HLA-B, HLA-C, HLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRB1, HLA-DRB3, HLA-DRB4 and HLA-DRB5 were 98.1%, 97.0% and 98.1%, 99.0%, 98.6%, 98.8%, 97.6%, 96.0%, 99.1%, 90.0% and 91.7%, respectively. Across all loci, the majority of the discordance was due to allele dropout. The high cost of NGS HLA genotyping per experiment likely prevented the retyping of initially failed HLA loci. Despite the high HLA genotype concordance rates of the software, there remains room for improvement in the assembly of more accurate consensus DNA sequences by NGS HLA genotyping software.

Out-of-pocket expenditures and care time for children with Down Syndrome: A single-hospital study in Mexico City.

AIM: To examine the burden of out-of-pocket household expenditures and time spent on care by families responsible for children with Down Syndrome (DS). METHODS: A cross-sectional analysis was performed after surveying families of children with DS. The children all received medical care at the Hospital Infantil de México Federico Gomez (HIMFG), a National Institute of Health. Data were collected on out-of-pocket household expenditures for the medical care of these children. The percentage of such expenditure was calculated in relation to available household expenditure (after subtracting the cost of food/housing), and the percentage of households with catastrophic expenditure. Finally, the time spent on the care of the child was assessed. RESULTS: The socioeconomic analysis showed that 67% of the households with children with DS who received medical care in the HIMFG were within the lower four deciles (I-IV) of expenses, indicating a limited ability to pay for medical services. Yearly out-of-pocket expenditures for a child with DS represented 27% of the available household expenditure, which is equivalent to $464 for the United States dollars (USD). On average, 33% of families with DS children had catastrophic expenses, and 46% of the families had to borrow money to pay for medical expenses. The percentage of catastrophic expenditure was greater for a household with children aged five or older compared with households with younger children. The regression analysis revealed that the age of the child is the most significant factor determining the time spent on care. CONCLUSIONS: Some Mexican families of children with DS incur substantial out-of-pocket expenditures, which constitute an economic burden for families of children who received medical care at the HIMFG.

Implicaciones obstétricas de los tratamientos escisionales de cérvix por lesiones premalignas / Excisional treatment for cervical dysplasia and obstetrics outcomes

Objetivo: Analizar los resultados obstétricos en mujeres con antecedente de tratamiento escisional de cérvix y si existen diferencias en los mismos según la técnica de conización empleada: LEEP (procedimiento de escisión cervical con asa de diatermia) o LLETZ (escisión de la zona de transformación [TZ] con asa de diatermia). Material y métodos: Estudio observacional retrospectivo en el que se han incluido las conizaciones realizadas entre 2007 y 2014 y las siguientes variables: edad materna, paridad, tabaquismo, parto, aborto, intervalo entre la conización y el parto, tipo de parto, rotura prematura de membranas pretérmino (RPMP), edad gestacional y peso del recién nacido. Grupo control: 100 pacientes que habían quedado gestantes en el mismo periodo de tiempo. Resultados: Cincuenta y tres (7,9%) pacientes quedaron gestantes tras la conización, de estas 4(7,5%) abortaron y 49(92,5%) tuvieron un parto. Se observó mayor tasa de prematuridad (18 vs. 8%, p=0,049) y de RPMP (22,4 vs. 3%, p=0,001) en el grupo de conización. En relación a la técnica empleada, hubo más casos de prematuridad en el grupo de LEEP frente al de LLETZ (25 vs. 10,5%), RR=1,7 (IC 95%:1,1-2,9), no encontrando diferencias en cuanto a la RPMP y el bajo peso. Por otro lado, también se observó mayor proporción de cesárea en el grupo de conización frente al control (38,8 vs. 20%) (57%-LEEP vs. 5,3%-LLETZ, p=0,013). Discusión: Es importante hacer un manejo conservador en mujeres con deseos genésicos y lesiones cervicales premalignas y seleccionar correctamente a las pacientes candidatas a conización. Cuando se utilizó LEEP los resultados obstétricos (en términos de prematuridad y RPMP) son peores frente a las gestantes en las que se realizó LLETZ

Objective: The aim of our study was to analyse the obstetric outcomes in women with a history of excisional treatment for cervical intraepithelial neoplasia and whether there were differences according to the methods of treatment used (loop electrosurgical excision procedure [LEEP] vs. large loop excision of the transformation zone [LLETZ]). Material and methods: A retrospective cohort study was conducted on patients who underwent conization between 2007 and 2014. Outcome measures included maternal age, parity, smoking, childbirth, abortion, interval between conization and delivery, mode of delivery, preterm prelabour rupture of the membranes (PPROM), gestational age and birth weight. A group of 100 patients who had been pregnant during the same period was used as a control group. Results: 53 (7.9%) patients became pregnant after conization, resulting in 4 (7.5%) miscarriages and 49 (92.5%) deliveries. Increased rate of preterm deliveries (18 vs. 8%, p=.048) and PPROM (22.4 vs. 3%, p=.001) was observed in the conization group. According to technique used, there were more cases of prematurity in the LEEP group against LLETZ (25 vs. 10.5%), RR=1.7 (95% CI: 1.1-2.9), no differences were found in PPROM and low weight. On the other hand, a higher proportion of caesareans was also observed in the conization group versus the control group (38.8 vs. 20%) (57%: LEEP vs. 5.3%: LLETZ, p=.013). Discussion: Conservative management and appropriate selection of candidates for conization are important in women with premalignant cervical lesions who wish to become pregnant. Worse results were observed in LEEP group (preterm delivery and PPROM) compared to women who underwent LLETZ

Eficacia de una intervención educativa para mejorar el conocimiento de las/os enfermeras/os sobre el sondaje gástrico en pediatría / Effectiveness of an educational intervention to improve nurses' knowledge on pediatric nasogastric intubation

Objetivo. Eficacia de una intervención educativa para mejorar el conocimiento de las/os enfermeras/os sobre el sondaje gástrico en pediatría. Métodos. Estudio multicéntrico cuasi experimental pre- y postest mediante intervención educativa teórica basada en la evidencia bibliográfica y la evaluación de su eficacia a los 4 meses. Resultados. Cuestionarios entregados: 1019. Válidos: 557 (54,66 %) pretest, 246 (24,14 %) postest. Las respuestas pre- y postest fueron que el riesgo implícito que conllevaba siempre el procedimiento había sido percibido por el 53,2 % y aumentó al 70,7 % (p < 0,001). El 4,3 % elegía el tamaño de la sonda gástrica (SG) mediante tablas y se elevó al 24,6 % (p < 0,001). La longitud de la SG por introducir medida mediante el método nariz-oreja-distancia media xifoides-ombligo (nose-ear-mid-umbilicus; NEMU, por sus siglas en inglés) ascendió del 34,2 % al 81,3 % (p < 0,001). La comprobación de la ubicación de la SG previa a su uso pasó del 73,1 % al 86,5 % (p < 0,001). La comprobación de los cinco correctos (paciente, medicamento, dosis, vía y hora) previa a la utilización de la SG aumentó del 85,6 % al 91 %. Como métodos de comprobación, mejoró la percepción de inseguridad de la auscultación del 11,7 % al 31,1 % (p < 0,001), y disminuyó su uso del 95,1 % al 81,6 %. Creció la percepción de seguridad de la medición del pH gástrico del 71,3 % al 91,1 % (p < 0,001), y aumentó su uso del 7,6 % al 54,3 % (p < 0,001). Conclusiones. La intervención educativa resultó eficaz para incrementar el conocimiento de enfermería sobre el sondaje gástrico pediátrico.

Objective. To establish the effectiveness of an educational intervention to improve nurses' knowledge on pediatric nasogastric intubation. Methods. Multicenter, quasi-experimental, pre- and post-test study using a theoretical educational intervention based on bibliographic evidence and assessment of its effectiveness after 4 months. Results. Delivered questionnaires: 1019. Valid questionnaires: 557 (54.66 %) pre-test and 246 (24.14 %) post-test. Pre- and post-test answers indicated that the implied risk always entailed by the procedure had been perceived by 53.2 % and then increased to 70.7 % (p < 0.001). Nasogastric (NG) tube size was chosen using tables by 4.3 % of participants, and increased to 24.6 % (p < 0.001). The length of NG tube to be inserted as measured by the nose-ear-midumbilicus distance (NEMU) method increased from 34.2 % to 81.3 % (p < 0.001). Confirmation of NG tube placement prior to use increased from 73.1 % to 86.5 % (p < 0.001). Confirmation of the five rights (patient, drug, dosage, route, and timing) prior to NG tube use increased from 85.6 % to 91 %. In relation to confirmation methods, the perception that auscultation was unsafe improved from 11.7 % to 31.1 % (p < 0.001), and its use reduced from 95.1 % to 81.6 %. The perception that the measurement of gastric pH was safe increased from 71.3 % to 91.1 % (p < 0.001), and its use rose from 7.6 % to 54.3 % (p < 0.001). Conclusions. The educational intervention was effective to increase nurses' knowledge on pediatric nasogastric intubation.