ABSTRACT
The present study aimed to compare the adhesion and proliferation of human periodontal ligament fibroblasts (hPDL) in transverse sections of the teeth sealed with two different obturation techniques, BioRoot RCS/hydraulic obturation (HO) and AH-Plus/continuous-wave condensation (CWC). The techniques were tested using an in vitro model to simulate the interaction between periodontal tissues and the materials. The root canals were instrumented and sterilized. A total of 15 samples were obturated with BioRoot RCS/HO and 15 samples with AH-Plus/CWC. Then, roots were sectioned to obtain obturated teeth slices, and hPDL cells were seeded onto the root slices. The results were obtained at intervals of 4 and 24h for cell adhesion; and at 3,7,14, and 21 days for cell proliferation. Empty cell culture plates were use as controls. The cell adhesion was increased at 4 and 24h for both groups, with an increased response observed in the BioRoot RCS/HO group (p<0.05). The difference in cell proliferation was also found between experimental groups. After 14 days of culture, BioRoot RCS/HO group showed an increase response than control and AH-Plus/CWC groups (p<0.05), and after 21 days both groups behaved better than control group, with an increased response observed in the BioRoot RCS/HO group. This study demonstrated that both root canal sealers allow the attach and growth of periodontal ligament fibroblasts, with an increased biological response in the BioRoot RCS/HO group.
El presente estudio se enfocó en comparar la adhesión y proliferación de fibroblastos de ligamento periodontal humano (hPDL) en secciones transversales de raíces previamente obturadas con dos técnicas de obturación diferentes: obturación hidráulica empleando cono único de gutapercha y BioRoot RCS como sellador (HO), y obturación de condensación de onda continua y AH-Plus como sellador (CWC). Los selladores se usaron en un modelo in vitro que simula la interacción entre los tejidos periodontales y los materiales de obturación. Los conductos radiculares fueron instrumentados, esterilizados y obturados. La muestra se compuso de un total de 15 raíces con la técnica BioRoot RCS/HO y 15 raíces con la técnica AH-Plus/CWC. Las células de hPDL fueron sembradas en condiciones estándar de cultivo sobre las raíces seccionadas. Los resultados fueron obtenidos a intervalos de 4 y 24h para adhesión celular, y a los 3,5,7,14 y 21 días de cultivo para proliferación celular. La adhesión celular a las 4 y 24 horas mostró ser diferente para ambas técnicas en comparación con el grupo control, siendo más importante en el grupo BioRoot RCS/HO. La diferencia en la proliferación entre grupos se observó a los 14 días de cultivo, únicamente para el grupo BioRoot RCS/HO; Sin embargo para el día 21 ambas técnicas mostraron mayor proliferación celular que el grupo control, con mejor respuesta para el grupo BioRoot RCS/HO. Este estudio ha demostrado que ambos selladores de conductos permiten la adhesión y crecimiento de fibroblastos de ligamento periodontal, siendo el grupo BioRoot RCS/HO el que mostró mayor biocompatibilidad.
Subject(s)
Humans , Pit and Fissure Sealants/analysis , Materials Testing , Periodontal Ligament , Receptors, Aryl HydrocarbonABSTRACT
The ectopic calcifications of non-mineralized tissues can occur in several forms throughout life, such as pulpal calcification. The presence of pulp stones is a challenge in endodontic treatment because they partially or fully obliterate the pulp chamber hindering access to root canals and their subsequent shaping. This study aimed to determine their crystallographic properties and evaluate the capacity of citric acid (CA) and ethylenediaminetetraacetic acid (EDTA) to promote the demineralization of pulp calcifications. The samples were obtained from patients with indications of endodontic treatment, and the radiographic examination was suggestive of pulp stone in at least one permanent tooth. The samples were isolated and analyzed by scanning electron microscopy/energy-dispersive x-ray spectroscopy (SEM/EDX). The Fourier Transform by high resolution-transmission electron microscopy, Raman microscopy, and X-ray diffraction (XRD) were used to identify the mineral phase and crystallographic characteristics. To evaluate the effect of CA and EDTA on the crystallinity of calcifications, they were submerged into these two individual solutions and the changes were assessed in situ by Raman spectroscopy. The SEM images obtained from calcifications demonstrated irregular morphologies. EDX of sample surfaces shows a high presence of oxygen, carbon, calcium, and phosphorous, however, other elements such as sodium, magnesium, nitrogen, chlorine, potassium, sulfur, and zinc were identified in less quantity. According to Raman, XRD, and high-resolution transmission electron microscopy, the predominant mineral phase identified in the pulpal calcification was a poor crystallinity apatite. According to in situ analyses, the effect of CA and EDTA was observed on the signals of PO4 3- and CH2 groups corresponding to inorganic and organic components. The changes with CA were evident at 7 min while the effect of EDTA was observed until 15 min of treatment. All results indicate that pulp stones have a heterogeneous composition principally composed of apatite with low crystallinity. The solubility of these pathological minerals is adequate using solutions such as EDTA or CA; however, the effectivity depends on the mineralization grade of calcifications, time, and concentration of exposition to this chemical.
Subject(s)
Calcinosis , Dental Pulp Calcification , Humans , Edetic Acid/pharmacology , Citric Acid , Microscopy, Electron, Scanning , Minerals/analysis , ApatitesABSTRACT
OBJETIVO: Comparar, en un estudio experimental in vitro, el porcentaje de áreas no obturadas entre la técnica de compactación lateral en frío (TCL) y la técnica de compactación lateral con ultrasonido (TCLU). MATERIALES Y MÉTODOS: El estudio fue realizado en una muestra de 68 dientes humanos extraídos. Se asignaron de manera aleatoria en dos grupos de 34 dientes cada uno. Todos los dientes se cortaron a 16±1 mm de longitud desde el ápice hacia la corona, se instrumentaron con el sistema rotatorio Protaper Next (Dentsply) y se realizó protocolo de irrigación final con NaOCl al 2.5% y EDTA al 17% neutralizados con suero fisiológico entre ellos. El Grupo I se obturó con TCL y el Grupo II con TCLU. En ambos grupos se utilizó Sealapex como cemento. Los dientes fueron seccionados horizontalmente a 2, 4, 6 y 8 mm desde el ápice; cada corte se observó con un microscopio estereoscópico (56X) y se tomaron fotografías. Se calculó el porcentaje de área obturada con gutapercha, cemento y de áreas no obturadas, utilizando el programa ImageJ. El análisis de los resultados se llevó acabo mediante la suma de rangos de Wilcoxon. RESULTADOS: El grupo TCLU presentó un mayor porcentaje de gutapercha en el conducto radicular en comparación con el grupo TCL (94.4% y 87.4% respectivamente), menor porcentaje de áreas no obturadas (1.3% y 4.2%), y menor porcentaje de áreas ocupadas con cemento (3.8% y 7.2%). Las diferencias fueron estadísticamente significativas (P<0.05).CONCLUSIÓN: Los resultados de este estudio muestran que la modificación de la técnica de compactación lateral con ultrasonido descrita en esta investigación presenta resultados in vitro superiores a TCL
AIM: To compare, in an Experimental in vitro study, the percentage of non-filled areas between cold lateral compaction technique (CLT) and lateral compaction with ultrasonic technique (LUT). METHODS: The study was made on in a sample of 68 extracted human teeth. They were randomized into two groups of 34 teeth each. All teeth were cut 16±1 mm from the apex and instrumented with the Protaper Next rotary system (Dentsply) and a final irrigation protocol was performed with NaOCl 2.5% and EDTA 17% neutralized with normal saline solution between them. Group I was filled with CLT and Group II with LUT. In both groups Sealapex was used as a cement. The teeth were sectioned horizontally at 2, 4, 6 and 8 mm from the apex; slices were the viewed through a stereomicroscope (56X) and pictures were taken. The percentage of gutta-percha filled area, percentage of non-filled area and percentage of sealer filled area were calculated for each section using the ImageJ program. Statistical analysis was performed using the Wilcoxon Rank-Sum test. RESULTS: LUT had a higher percentage of gutta-percha in the root canal compared to CLT (94.4% and 87.4% respectively), lower percentage of non-filled area (1.3% and 4.2% respectively), and lower percentage of area occupied by sealer (3.8 % and 7.2% respectively). The differences were statistically significant(P <0.05).CONCLUSION: The results of this study showed that the modification of lateral compaction technique with ultrasonic described in this investigation presents in vitro results superior to CLT
Subject(s)
Humans , Root Canal Obturation/methods , Ultrasonic Therapy/methods , Gutta-Percha/therapeutic use , Dental Cements/therapeutic use , Materials Testing , Reproducibility of Results , Calcium Hydroxide/therapeutic use , Salicylates/therapeutic useABSTRACT
RESUMEN En la práctica odontológica, en la toma de radiografías no es común la colocación de dosímetros en los pacientes, sin embargo, por medio de la dosimetría podemos mantener un mejor control de la cantidad de radiación que es emitida a los pacientes, de la dosis de radiación y de la sensibilidad del tejido frente a la radiación, para así prevenir en algún momento los posibles efectos nocivos de la radiación ionizante; puesto que en odontología se encuentran por debajo las dosis umbral requeridas para producir reacciones tisulares (efectos deterministas), sin embargo, los efectos estocásticos pueden desarrollarse y presentarse con cualquier dosis de radiación. En este estudio nos enfocaremos en las ortopantomografías que son auxiliares de diagnóstico previo a un tratamiento odontológico.
ABSTRACT In dentistry, the use of dosimeters is not frequently to evaluate the patient radiation; however, through dosimetry, we can maintain a better control of the amount of radiation emitted to patients, radiation doses and the tissue radiosensitivity, to prevent at any time the posible harmful effects of ionizing radiation (deterministic effects), however the stochastic effects can be developed and presented with any doce of radiation. In this study we will focus on orthopantomographies that are diagnostic auxiliaries prior to dental treatment.
ABSTRACT
Se presenta en la Clínica de Endodoncia de la División de Estudios de Postgrado e Investigación (DEPeI) de la Facultad de Odontología (UNAM) una paciente diagnosticada con mucormicosis rinocerebral; en este artículo se presentan las variaciones en el manejo clínico de esta paciente y la importancia de mantener el mayor número de órganos dentales en boca para una mejor estabilidad del obturador palatino, tanto para las funciones de fonación como de masticación adecuadas.
A patient previously diagnosed with cerebral mucormycosis attended the Endodontics Clinic of the Graduate and Research School, National School of Dentistry, National University of Mexico (UNAM). This article presents variations in the clinical handling of the patient; stress is made on the importance of preserving the greatest number of teeth in the mouth to thus achieve better stability of the palatal obturator and establish suitable phonation and mastication functions.
