High Genetic Similarity Among Salmonella Heidelberg Isolated from Poultry Farms, Wild Animals, Beef, Poultry and Pork Meat, and Humans in Brazil

Salmonellosis is an important gastrointestinal infection in humans and cause of foodborne outbreaks in the world. In this context, molecular characterization is essential to understand how the strains circulate. The aim of this study was to evaluate the genotypic distribution of S. Heidelberg according to the source of isolation. The genetic relatedness of the S. Heidelberg isolates was determined by pulsed-field gel electrophoresis (PFGE). The most prevalent pulsotypes of cluster A were BRJF6X01.006 (27/95 = 28,42%) related between 1995 and 2011 in broilers, poultry meat and poultry farms, meat product and human, and BRJF6X01.001 (21/95 = 22,10%) related between 2011 and 2017 in wild animals, broilers, poultry meat, poultry farms, meat product, animal feed, and pork meat. The pulsotype BRJF6X01.001 shows a high distribution in the environmental and productive chain. The degree of similarity between pulsotypes BRJF6X01.006 and BRJF6X01.001 is 88%. To ensure the safety of human and animal health, holistic approaches, including surveillance of Salmonella throughout the environment and in the production chain, together with control measures, are critical. As transmission of Salmonella from food producing animals to wildlife and to the environment is considered potential public health problem, information on the survival and persistence of Salmonella in the environment and in potential reservoirs is of considerable importance.(AU)

Comparison of cell invasion, macrophage survival and inflammatory cytokines profiles between Salmonella enterica serovars Enteritidis and Dublin from Brazil.

AIMS: This study compared the capacity of strains of Salmonella enterica serovars Enteritidis and Dublin isolated in Brazil to invade epithelial cells, to be internalized by and survive within macrophages, and to stimulate cytokine release in vitro. METHODS AND RESULTS: Both serovars infected 75 and 73% Caco-2 (human) and MDBK (bovine) epithelial cells respectively. Salmonella Dublin and S. Enteritidis (i) were internalized at the respective rates of 79·6 and 65·0% (P ≤ 0·05) by U937 (human) macrophages, and 70·4 and 66·9% by HD11 (chicken) macrophages; and (ii) multiplied at the respective rates of 3·2- and 2·7-fold within U937 cells, and 1·9- and 1·1-fold (P ≤ 0·05) within HD11 cells respectively. Seventy per cent of 10 S. Dublin strains stimulated IL-8 production, while 70% of S. Enteritidis strains enhanced production of IL-1ß, IL-6, IL-8, IL-10, IL-12p70 and TNF in Caco-2 cells. CONCLUSIONS: Compared with S. Enteritidis, S. Dublin had stronger ability to survive within macrophages and induced weak cytokine production, which may explain the higher incidence of invasive diseases caused by S. Dublin in humans. SIGNIFICANCE AND IMPACT OF THE STUDY: This study compared S. enterica serovars Enteritidis and Dublin to provide comparative data about the profile of the two serovars in cells from humans, the common host and their respective natural animal hosts and vice versa in order to check the differences between these two phylogenetically closely related serovars that share antigenic properties but present different phenotypic behaviours.

High Prevalence of Multidrug-Resistant Nontyphoidal Salmonella Recovered from Broiler Chickens and Chicken Carcasses in Brazil

The extensive use of antimicrobial agents has contributed to the emergence of antimicrobial resistance and multidrug resistance (MDR) in Salmonella, an important zoonotic pathogen that causes outbreaks and sporadic cases of gastroenteritis in humans. The study aimed to investigate the antimicrobial resistance profile of Salmonella strains isolated from poultry in Brazil. A total of 230 Salmonella strains, isolated from cloacal swabs (n=56) and broiler carcasses swabs (n=174) before and after chilling from slaughterhouses under Federal Inspection Service within the period 2012-2017, were analyzed. Serotyping and antibiotic susceptibility testing were performed on all the isolates. Serotyping results showed that 41% of the strains were Salmonella Heidelberg, 29% S. Minnesota, 12% S. Saintpaul, 6.5% S. Enteritidis, 3.9% S. Anatum, 2.2% S. Cerro, 2.2% S. Senftenberg, 1.7% S. Newport, 0.4% S. Ealing, 0.4% S. O:4,5 and 0.4% S. O:9,12. MDR rates of the isolates were 67.4%. S. Heidelberg 89.5%, S. Minnesota 51.5%, S. Saintpaul 82.1%, S. Anatum 66.7%, S. Cerro 60%, S. Senftenberg 40%. Out of the 230 strains, 41.3% presented resistance to Penicillins + beta-lactamase inhibitor, Penicillin, 1st and 2nd Generation Cephalosporin, 3rd and 4th Generation Cephalosporin, Tetracycline and Sulfonamide. Salmonella Heidelberg, S. Saintpaul, S. Anatum, S. Cerro, S. Senftenberg and S. Minnesota were isolated after chilling tank highlighting a food safety concern for the industry of poultry and poultry products indicating a risk to collective health. The high prevalence of MDR nontyphoidal Salmonella obtained in this study limit the options available to treat infectious disease in humans and animals.

Qualitative and Quantitative Analysis of Salmonella spp. in Broilers Technological Processing and Determination of a Performance Objective (PO) for Frozen Chicken Breast

The present study investigated the frequency, level of contamination and serotyping of Salmonella strains isolated from broiler flocks in different processing sites and the fulfillment of a Performance Objective (PO) in frozen chicken breasts, as a risk assessment to measure the efficacy of prevention and control programs applied to reduce the risk of Salmonella spp. in raw poultry meat that contribute to reach food safety and public health goals. From 1,800 samples of cloacal swabs, carcasses before and after immersion chilling and frozen breasts derived from 20 broiler flocks slaughtered at two processing plants located in the mid-west and southern regions of Brazil, 278 samples were positive for Salmonella spp. by polymerase chain reaction (PCR) automated BAX System (DUPONT QUALICOM, USA), and 118 were enumerated by miniaturized most probable number technique. 122 Salmonella spp. strains were serotyped at the National Reference Laboratory of Cholera and Enteric Diseases of Oswaldo Cruz Institute Foundation (FIOCRUZ), showing a dominance of Salmonella Minnesota in every processing steps of the slaughterhouse located in the Brazilian mid-west region. Only 1 lot failed to reach the expected result for the Performance Objective (PO), using a maximum of 10% positivity acceptance for Salmonella spp. in frozen chicken breasts. Qualitative and quantitative results combined may be considered an effective tool to evaluate the effect of prevention and control programs for Salmonella spp. on the safety of the final product.

Qualitative and Quantitative Analysis of Salmonella spp. in Broilers Technological Processing and Determination of a Performance Objective (PO) for Frozen Chicken Breast

The present study investigated the frequency, level of contamination and serotyping of Salmonella strains isolated from broiler flocks in different processing sites and the fulfillment of a Performance Objective (PO) in frozen chicken breasts, as a risk assessment to measure the efficacy of prevention and control programs applied to reduce the risk of Salmonella spp. in raw poultry meat that contribute to reach food safety and public health goals. From 1,800 samples of cloacal swabs, carcasses before and after immersion chilling and frozen breasts derived from 20 broiler flocks slaughtered at two processing plants located in the mid-west and southern regions of Brazil, 278 samples were positive for Salmonella spp. by polymerase chain reaction (PCR) automated BAX System (DUPONT QUALICOM, USA), and 118 were enumerated by miniaturized most probable number technique. 122 Salmonella spp. strains were serotyped at the National Reference Laboratory of Cholera and Enteric Diseases of Oswaldo Cruz Institute Foundation (FIOCRUZ), showing a dominance of Salmonella Minnesota in every processing steps of the slaughterhouse located in the Brazilian mid-west region. Only 1 lot failed to reach the expected result for the Performance Objective (PO), using a maximum of 10% positivity acceptance for Salmonella spp. in frozen chicken breasts. Qualitative and quantitative results combined may be considered an effective tool to evaluate the effect of prevention and control programs for Salmonella spp. on the safety of the final product.(AU)

High Prevalence of Multidrug-Resistant Nontyphoidal Salmonella Recovered from Broiler Chickens and Chicken Carcasses in Brazil

The extensive use of antimicrobial agents has contributed to the emergence of antimicrobial resistance and multidrug resistance (MDR) in Salmonella, an important zoonotic pathogen that causes outbreaks and sporadic cases of gastroenteritis in humans. The study aimed to investigate the antimicrobial resistance profile of Salmonella strains isolated from poultry in Brazil. A total of 230 Salmonella strains, isolated from cloacal swabs (n=56) and broiler carcasses swabs (n=174) before and after chilling from slaughterhouses under Federal Inspection Service within the period 2012-2017, were analyzed. Serotyping and antibiotic susceptibility testing were performed on all the isolates. Serotyping results showed that 41% of the strains were Salmonella Heidelberg, 29% S. Minnesota, 12% S. Saintpaul, 6.5% S. Enteritidis, 3.9% S. Anatum, 2.2% S. Cerro, 2.2% S. Senftenberg, 1.7% S. Newport, 0.4% S. Ealing, 0.4% S. O:4,5 and 0.4% S. O:9,12. MDR rates of the isolates were 67.4%. S. Heidelberg 89.5%, S. Minnesota 51.5%, S. Saintpaul 82.1%, S. Anatum 66.7%, S. Cerro 60%, S. Senftenberg 40%. Out of the 230 strains, 41.3% presented resistance to Penicillins + beta-lactamase inhibitor, Penicillin, 1st and 2nd Generation Cephalosporin, 3rd and 4th Generation Cephalosporin, Tetracycline and Sulfonamide. Salmonella Heidelberg, S. Saintpaul, S. Anatum, S. Cerro, S. Senftenberg and S. Minnesota were isolated after chilling tank highlighting a food safety concern for the industry of poultry and poultry products indicating a risk to collective health. The high prevalence of MDR nontyphoidal Salmonella obtained in this study limit the options available to treat infectious disease in humans and animals.(AU)

Imagens do ato de amamentar como cuidado em saúde: a percepção das próprias nutrizes / Images of the act of breastfeeding as health care: the perception of the mothers themselves

Objetivo: analisar a percepção das nutrizes sobre o ato de aleitar, a partir da própria imagem fotográfica. Método: estudo qualitativo do tipo descritivo-exploratório, realizado com 10 puérperas numa maternidade de risco habitual. Coleta de dados ocorreu após apresentação das fotografias das próprias nutrizes, amamentando na sala de parto e puerpério, com entrevistas semiestruturadas, realizadas no domicílio das participantes e, posteriormente, estas foram submetidas à análise de conteúdo temática. Resultados: as imagens tridimensionais dessas mulheres vivenciando a lactação, refletiu a rede de cuidados à amamentação no pré-natal, parto e puerpério presente na atuação dos profissionais de saúde, tendo em vista o alcance do sucesso da amamentação. Conclusão: a percepção da nutriz acerca da própria imagem corporal durante o aleitamento, foi considerada a expressão de um momento singular resultante do processo profissional de cuidado em saúde para o sucesso da vivência da amamentação.

Objective: to analyze the mothers' perception of the act of breastfeeding, from the photographic image itself. Method: qualitative study of the descriptive-exploratory type, carried out with 10 puerperal in a maternity unit at usual risk. Data collection occurred after presenting the photographs of the mothers themselves, during breastfeeding in the delivery room and puerperium, using a semi-structured interview conducted in their homes and later, these were submitted to thematic content analysis. Results: the three-dimensional images of these women experiencing lactation reflected the network of breastfeeding care in the prenatal, delivery and puerperium present in the health professionals’ performance, in view of the success of breastfeeding. Conclusion: the nurturers’ perception about her own body image during breastfeeding was considered the expression of a singular moment resulting from the professional process of health care for the success of breastfeeding experience.

Staphylococcus spp. isolated from wild birds apprehended in the local illegal trade in Rio de Janeiro, Brazil, and relevance in public health.

This work aimed to investigate the prevalence of Staphylococcus in wild birds seized in illegal trade and their antimicrobial resistance patterns. Cloacal samples were obtained from 109 wild birds apprehended in the street markets in Rio de Janeiro, Brazil. Staphylococcus spp. were phenotypically and genotypically identified, and resistance profile was evaluated according to Clinical and Laboratory Standards Institute guidelines and by polymerase chain reaction of mecA and blaZ genes. Staphylococcus was detected in 45·9% (50/109) of the cloacal swab samples, and 39 (78·0%) isolates were resistant to one or more of the nine antimicrobials tested and were also positive to mecA (12/39) or blaZ genes (14/39). High percentage of resistance was detected to ampicillin, oxacillin, cefoxitin, clindamycin and tetracycline, with the absence of resistance to vancomycin. Wild birds captured and submitted to captive stress conditions of illegal trade market of Brazil may have an important role as reservoirs of Staphylococcus spp. and its antimicrobial resistance mechanisms. The significance of this study is revealed by the zoonotic and pathogenic potential of staphylococci and that impact to public health and requires monitoring polices of wild birds health in tropical areas. SIGNIFICANCE AND IMPACT OF THE STUDY: The isolation of Staphylococcus species that are not commonly isolated from wild bird faeces, the relatively high proportion of strains showing degrees of resistance to ß-lactamics, lincosamides and tetracycline, and also the presence of mecA and blaZ genes that has been associated with multidrug phenotype reveal its public health relevance and zoonotic potential. Consequently, this represents an important route to transmission of this pathogen and its antimicrobial resistance mechanisms throughout national and international frontiers fostered by the illegal trade of wild animals and close contact with humans.

Comparative genome analysis and characterization of the Salmonella Typhimurium strain CCRJ_26 isolated from swine carcasses using whole-genome sequencing approach.

Salmonella pathogenicity relies on virulence factors many of which are clustered within the Salmonella pathogenicity islands. Salmonella also harbours mobile genetic elements such as virulence plasmids, prophage-like elements and antimicrobial resistance genes which can contribute to increase its pathogenicity. Here, we have genetically characterized a selected S. Typhimurium strain (CCRJ_26) from our previous study with Multiple Drugs Resistant profile and high-frequency PFGE clonal profile which apparently persists in the pork production centre of Rio de Janeiro State, Brazil. By whole-genome sequencing, we described the strain's genome virulent content and characterized the repertoire of bacterial plasmids, antibiotic resistance genes and prophage-like elements. Here, we have shown evidence that strain CCRJ_26 genome possible represent a virulence-associated phenotype which may be potentially virulent in human infection. SIGNIFICANCE AND IMPACT OF THE STUDY: Whole-genome sequencing technologies are still costly and remain underexplored for applied microbiology in Brazil. Hence, this genomic description of S. Typhimurium strain CCRJ_26 will provide help in future molecular epidemiological studies. The analysis described here reveals a quick and useful pipeline for bacterial virulence characterization using whole-genome sequencing approach.

A comprehensive survey of Aeromonas sp. and Vibrio sp. in seabirds from southeastern Brazil: outcomes for public health.

AIMS: To perform a microbiological survey regarding the presence, prevalence and characterization of Aeromonas sp. and Vibrio sp. in debilitated wrecked marine birds recovered from the centre-north coast of the state of Rio de Janeiro, Brazil. METHODS AND RESULTS: Swabs obtained from 116 alive and debilitated wrecked marine birds, comprising 19 species, from the study area were evaluated by biochemical methods. Antimicrobial susceptibility tests and pathogenicity gene screening were performed for bacterial strains of public health importance. Vibrio sp. and Aeromonas sp. were identified, as well as certain pathogenic genes and resistance to selected antimicrobials. CONCLUSIONS: This study demonstrates that the identified bacteria, mainly Vibrio sp., are fairly prevalent and widespread among several species of seabirds and highlights the importance of migratory birds in bacterial dispersion. In addition, it demonstrates the importance of the bacterial strains regarding their pathogenic potential. Therefore, seabirds can act as bacterial reservoirs, and their monitoring is of the utmost importance in a public health context. SIGNIFICANCE AND IMPACT OF THE STUDY: The study comprehensively evaluates the importance of seabirds as bacteria of public health importance reservoirs, since birds comprising several pathogenic bacterial species were evaluated.

Genetic diversity, anti-microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil.

Salmonella Dublin is strongly adapted to cattle causing enteritis and/or systemic disease with high rates of mortality. However, it can be sporadically isolated from humans, usually causing serious disease, especially in patients with underlying chronic diseases. The aim of this study was to molecularly type S. Dublin strains isolated from humans and animals in Brazil to verify the diversity of these strains as well as to ascertain possible differences between strains isolated from humans and animals. Moreover, the presence of the capsular antigen Vi and the plasmid profile was characterized in addition to the anti-microbial resistance against 15 drugs. For this reason, 113 S. Dublin strains isolated between 1983 and 2016 from humans (83) and animals (30) in Brazil were typed by PFGE and MLVA. The presence of the capsular antigen Vi was verified by PCR, and the phenotypic expression of the capsular antigen was determined serologically. Also, a plasmid analysis for each strain was carried out. The strains studied were divided into 35 different PFGE types and 89 MLVA-types with a similarity of ≥80% and ≥17.5%, respectively. The plasmid sizes found ranged from 2 to >150 kb and none of the strains studied presented the capsular antigen Vi. Resistance or intermediate resistance was found in 23 strains (20.3%) that were resistant to ampicillin, ciprofloxacin, chloramphenicol, imipenem, nalidixic acid, piperacillin, streptomycin and/or tetracycline. The majority of the S. Dublin strains studied and isolated over a 33-year period may descend from a common subtype that has been contaminating humans and animals in Brazil and able to cause invasive disease even in the absence of the capsular antigen. The higher diversity of resistance phenotypes in human isolates, as compared with animal strains, may be a reflection of the different anti-microbial treatments used to control S. Dublin infections in humans in Brazil.

Prevalence and identification of Salmonella spp. in water buffaloes from São Paulo State, Brazil / Prevalência e identificação de Salmonella spp. em búfalos do Estado de São Paulo, Brasil

O objetivo do estudo foi investigar a prevalência de Salmonella spp. em amostras de fezes de búfalos do estado de São Paulo, Brasil, e identificar os sorotipos isolados. Foram examinadas 116 amostras de suabes retais de búfalos das raças Jafarabadi e Murrah, coletadas em triplicata, em seis propriedades rurais localizadas nas regiões Central, Centro-Oeste e Nordeste do estado de São Paulo, Brasil. Para avaliar a presença de Salmonella spp., foram utilizados três diferentes caldos de enriquecimento (caldo selenito cistina, caldo tetrationado Muller-Kauffmann e caldo Rappaport-Vassiliadis) e dois diferentes meios de cultura (ágar verde brilhante modificado e ágar XLT4). Das 116 amostras de suabes retais examinadas, oito amostras (6,90%; 8/116) foram positivas para Salmonella spp., incluindo quatro sorotipos: S. Panama (50%; 4/8), S. Agona (25%; 2/8) , S. Newport (12,5%; 1/8) e S. Saintpaul (12,5%; 1/8), todos isolados de búfalos sem sinais clínicos de salmonelose, indicando a importância dos animais assintomáticos como fonte de infecção para outros animais e seres humanos. Das seis propriedades rurais avaliadas, apenas em duas fazendas (33,3%; 2/6) não foi detectada Salmonella spp. O uso de mais de um caldo de enriquecimento seletivo e de mais de um meio de cultura é indicado para o isolamento de Salmonella.(AU)

Prevalence and identification of Salmonella spp. in water buffaloes from São Paulo State, Brazil / Prevalência e identificação de Salmonella spp. em búfalos do Estado de São Paulo, Brasil

O objetivo do estudo foi investigar a prevalência de Salmonella spp. em amostras de fezes de búfalos do estado de São Paulo, Brasil, e identificar os sorotipos isolados. Foram examinadas 116 amostras de suabes retais de búfalos das raças Jafarabadi e Murrah, coletadas em triplicata, em seis propriedades rurais localizadas nas regiões Central, Centro-Oeste e Nordeste do estado de São Paulo, Brasil. Para avaliar a presença de Salmonella spp., foram utilizados três diferentes caldos de enriquecimento (caldo selenito cistina, caldo tetrationado Muller-Kauffmann e caldo Rappaport-Vassiliadis) e dois diferentes meios de cultura (ágar verde brilhante modificado e ágar XLT4). Das 116 amostras de suabes retais examinadas, oito amostras (6,90%; 8/116) foram positivas para Salmonella spp., incluindo quatro sorotipos: S. Panama (50%; 4/8), S. Agona (25%; 2/8) , S. Newport (12,5%; 1/8) e S. Saintpaul (12,5%; 1/8), todos isolados de búfalos sem sinais clínicos de salmonelose, indicando a importância dos animais assintomáticos como fonte de infecção para outros animais e seres humanos. Das seis propriedades rurais avaliadas, apenas em duas fazendas (33,3%; 2/6) não foi detectada Salmonella spp. O uso de mais de um caldo de enriquecimento seletivo e de mais de um meio de cultura é indicado para o isolamento de Salmonella.(AU)

Serotyping and genotyping of salmonella strains isolated from broilers, chicken carcasses before and after chilling, and frozen chicken breasts produced in the states of Mato Grosso do Sul and Santa Catarina, Brazil

The present study investigated the effectiveness of a single Salmonella prevention and control program applied in two different processing plants, located in the states of Mato Grosso do Sul (plant A) and Santa Catarina (Plant B), belonging to the same company, and identified Salmonella strain subtypes isolated from broilers, carcasses before and after chilling, and frozen chicken breasts. The Salmonella prevention and control program was 90% effective in plant A and 100% in plant B, considering a level of 10% positive samples per frozen chicken breast batch acceptable. A total of 128 strains were serotyped, being 10 from drag swabs, 31 from cloacal swabs, 83 from carcasses, and 4 from frozen chicken breasts. After serotyping analysis, 30 strains isolated at different processing steps and drag swabs, and three Salmonella Minnesota strains isolated in 2012 in plant A, were genotyped by PFGE. In plant A, the most frequently strain isolated was Salmonella Minnesota (90.35%), followed by Salmonella Newport (8.77%), and in Plant B, Salmonella Senftenberg (80%). Salmonella Minnesota strains were differentiated by PFGE into 19 pulsotypes distributed in three clusters. The phenotypic identification by serotyping of four strains diverged from their PFGE genotypic results. Most Salmonella Minnesota strains genotyped in plant A and the strains isolated from environmental samples in 2012 in the same broiler processing plant belong to a single cluster, confirming the dominance and persistence of this clone over time.

Serotyping and genotyping of salmonella strains isolated from broilers, chicken carcasses before and after chilling, and frozen chicken breasts produced in the states of Mato Grosso do Sul and Santa Catarina, Brazil

The present study investigated the effectiveness of a single Salmonella prevention and control program applied in two different processing plants, located in the states of Mato Grosso do Sul (plant A) and Santa Catarina (Plant B), belonging to the same company, and identified Salmonella strain subtypes isolated from broilers, carcasses before and after chilling, and frozen chicken breasts. The Salmonella prevention and control program was 90% effective in plant A and 100% in plant B, considering a level of 10% positive samples per frozen chicken breast batch acceptable. A total of 128 strains were serotyped, being 10 from drag swabs, 31 from cloacal swabs, 83 from carcasses, and 4 from frozen chicken breasts. After serotyping analysis, 30 strains isolated at different processing steps and drag swabs, and three Salmonella Minnesota strains isolated in 2012 in plant A, were genotyped by PFGE. In plant A, the most frequently strain isolated was Salmonella Minnesota (90.35%), followed by Salmonella Newport (8.77%), and in Plant B, Salmonella Senftenberg (80%). Salmonella Minnesota strains were differentiated by PFGE into 19 pulsotypes distributed in three clusters. The phenotypic identification by serotyping of four strains diverged from their PFGE genotypic results. Most Salmonella Minnesota strains genotyped in plant A and the strains isolated from environmental samples in 2012 in the same broiler processing plant belong to a single cluster, confirming the dominance and persistence of this clone over time.(AU)

Sorovares e perfil de suscetibilidade a antimicrobianos em Salmonella spp. isoladas de produtos de origem suína / Serovars and antimicrobial susceptibility of Salmonella spp. product isolated from swine

Foi analisado um total de 1824 cepas de Salmonella, isoladas de alimentos de origem suína, no período de janeiro/2005 a junho/2010. As cepas, provenientes de diferentes regiões do país, foram recebidas pelo Labent/IOC/FIocruz para caracterização antigênica conclusiva. Foram identificados 41 sorovares, destacando-se: Typhimurium, Derby, Enteritidis, Panama, Infantis e Anatum. Aspectos bacteriológicos e epidemiológicos relacionados a esses sorovares foram discutidos. O teste de suscetibilidade aos antimicrobianos foi realizado em 357 amostras, 257 (72%) foram resistentes a uma ou mais drogas, e destas, 31,9% mostraram-se multirresistentes. A variedade de sorovares observada neste estudo confirma o papel dos suínos na cadeia alimentar como importantes reservatórios de Salmonella, agravado ainda pelo elevado percentual de cepas resistentes a um ou mais antimicrobianos, alertando para uma condição de risco à saúde pública.

We analyzed a total of 1824 strains of Salmonella isolated from swine-origin foods from January/2005 to June/2010. The strains from different regions of the country were received by Labent/IOC/FIOCRUZ for conclusive antigenic characterization. We identified 41 serovars, of which these stood out: Typhimurium, Derby, Enteritidis, Panama, Infantis and Anatum. Bacteriological and epidemiological aspects related to these serovars were discussed. The antimicrobial susceptibility test was performed on 357 samples, 257 (72%) were resistant to one or more of these drugs and 31,9% were multiresistant. A variety of serovars were identified reinforcing the swine as an important reservoir of Salmonella in the food chain. The high rates of antimicrobial resistance obtained in this evaluation may represent a risk condition to human health.

Sorovares e perfil de suscetibilidade a antimicrobianos em Salmonella spp. isoladas de produtos de origem suína / Serovars and antimicrobial susceptibility of Salmonella spp. product isolated from swine

Foi analisado um total de 1824 cepas de Salmonella, isoladas de alimentos de origem suína, no período de janeiro/2005 a junho/2010. As cepas, provenientes de diferentes regiões do país, foram recebidas pelo Labent/IOC/FIocruz para caracterização antigênica conclusiva. Foram identificados 41 sorovares, destacando-se: Typhimurium, Derby, Enteritidis, Panama, Infantis e Anatum. Aspectos bacteriológicos e epidemiológicos relacionados a esses sorovares foram discutidos. O teste de suscetibilidade aos antimicrobianos foi realizado em 357 amostras, 257 (72%) foram resistentes a uma ou mais drogas, e destas, 31,9% mostraram-se multirresistentes. A variedade de sorovares observada neste estudo confirma o papel dos suínos na cadeia alimentar como importantes reservatórios de Salmonella, agravado ainda pelo elevado percentual de cepas resistentes a um ou mais antimicrobianos, alertando para uma condição de risco à saúde pública.(AU)

We analyzed a total of 1824 strains of Salmonella isolated from swine-origin foods from January/2005 to June/2010. The strains from different regions of the country were received by Labent/IOC/FIOCRUZ for conclusive antigenic characterization. We identified 41 serovars, of which these stood out: Typhimurium, Derby, Enteritidis, Panama, Infantis and Anatum. Bacteriological and epidemiological aspects related to these serovars were discussed. The antimicrobial susceptibility test was performed on 357 samples, 257 (72%) were resistant to one or more of these drugs and 31,9% were multiresistant. A variety of serovars were identified reinforcing the swine as an important reservoir of Salmonella in the food chain. The high rates of antimicrobial resistance obtained in this evaluation may represent a risk condition to human health.(AU)

Chicken feet bacteriological quality at 4 steps of technological processing.

The production of chicken feet is primarily intended for foreign markets, and there is still no specific legislation in Brazil that determines the quality standard of these products. The bacteriological quality of chicken feet was evaluated as a product for human consumption at different steps of the technological processes. Eighty broiler feet from 20 lots at 4 steps of processing were collected for quantitative analysis, total count of aerobic mesophilic bacteria, and determining the most probable number of coliforms and fecal coliforms. Thirty-eight pools of 15 broiler feet each from 19 lots were used for qualitative analysis and the isolation of Salmonella enterica spp. and Escherichia coli O157:H7. Escherichia coli O157:H7 was not found in any of the samples. Salmonella spp. were isolated in 68% (13/19) of the lots. The Salmonella Schwarzengrund serotype was found in 12 of the 13 lots of positive samples and the Salmonella Anatum and Salmonella Corvallis serotypes were identified in the remaining lot. Processing is effective in reducing contamination by mesophilic bacteria, coliforms, and Salmonella spp. in these products. This work constitutes the first study in Brazil on microbiological quality of chicken feet.

Incidence and antimicrobial resistance of enteropathogens isolated from an integrated aquaculture system.

AIMS: To evaluate an integrated aquaculture system, microbiological analyses of water used in this system were carried out and the incidence and antimicrobial resistance of enteropathogens were determined in the related ecosystem. METHODS AND RESULTS: Microbiological analysis was undertaken for Salmonella sp., Shigella sp., Vibrio sp. and Aeromonas sp. The disc-diffusion method was performed according to the Clinical and Laboratory Standards Institute. Water samples tested had 32.9% of faecal coliform rates (≤1600 per 100 ml) in accordance with WHO for pisciculture in wastewater. Salmonella spp. were detected in 14.5% of the samples. From a total of 33 strains, 15.1% were resistant to one or two antimicrobial drugs tested and multidrug resistance was not observed. Aeromonas spp. were identified in 91.6% of the samples. From a total of 416 strains, resistance to one antimicrobial class was observed in 66.3% and multidrug resistance in 37.7%. CONCLUSIONS: This system reflects the community profile, drawing attention to the circulation of pathogens, because the genes coding for resistance to classical antibiotics and broad spectrum are a public health problem. SIGNIFICANCE AND IMPACT OF THE STUDY: The reuse of water resources requires continuous monitoring as the system is subject to treatment failure, which can result in the spread of bacterial pathogens.

Antimicrobial resistance and subtyping of Salmonella enterica subspecies enterica serovar Enteritidis isolated from human outbreaks and poultry in southern Brazil.

To investigate antimicrobial resistance, 96 Salmonella enterica subspecies enterica serovar Enteritidis strains isolated from salmonellosis outbreaks and poultry-related products obtained in southern Brazil were analyzed. Macrorestriction patterns, obtained by pulsed-field gel electrophoresis and phage types, were assessed. Although 43.75% of samples were sensitive to all drugs tested, resistance to sulfonamide (34.37%), trimethoprim-sulfamethoxazole (25.00%), nalidixic acid (14.58%), streptomycin (2.08%), gentamicin, and tetracycline (1.04%) was identified. Furthermore, 89.60% of strains belonged to phage type 4, and a predominant pulsed-field gel electrophoresis genotype represented by 82.29% of the strains was identified, suggesting that a clonal group was distributed in poultry, food, and human isolates. Although it was not possible to associate strains from different sources, the occurrence of antimicrobial-resistant Salmonella Enteritidis strains supports the need to establish monitoring programs to identify the emergence of potential resistance patterns and to direct policies for use of these drugs in food-producing animals.