Rice vinasse treatment by immobilized Synechococcus pevalekii and its effect on Dunaliella salina cultivation.

The development of new strategies in microalgal studies represents an outstanding opportunity to mitigate environmental problems coupled with biomass production at a reduced cost. Here we present a combined bioprocess for the treatment of rice vinasse using immobilized cyanobacteria Synechococcus pevalekii in alginate beads followed by the use of the treated vinasse as a culture medium for Dunaliella salina biomass production. Cyanobacterial-alginate beads showed a chlorophyll a production of 0.68 × 10-3 mg bead-1 and a total carotenoid production of 0.64 × 10-3 mg bead-1. The first step showed a decrease in nitrate (91%), total solids (29%), and ions. Addition of treated vinasse into D. salina cultivation resulted in a significant increase in cell replication of about 175% (optimized cultivation). The use of natural seawater drastically reduced the medium cost to US$4.75 per m3 and the addition of treated vinasse has the potential to reduce it even more (up to 69%). This study not only provides an insight on the use of cyanobacteria for rice vinasse treatment but also demonstrates a promising lower-cost medium for marine microalgal biomass production with biotechnological purposes.

Modified soybean meal polysaccharide with high adhesion capacity to Salmonella.

Carbohydrates are known to act as analog receptors for bacteria and therefore are promising alternatives for the control and prevention of bacterial infections. The present study evaluated the chemical structure of modified soybean meal polysaccharides and their capacity to adhere enterobacteria (Salmonella Typhimurium) and to interfere with the bacteria adhesion to the known analogue receptors, using in vitro assays. For this, soybean meal suspensions were subjected to a thermochemical extraction process and structural analyses showed that the fraction with higher adhesion and adhesion-inhibition potential, SAP, was constituted by two types of polysaccharides: a partially depolymerized pectin, of high molar mass, composed of xylogalacturonan and rhamnogalacturonan regions (SAP1, 545.5 kDa), and a (1 → 4)-linked-ß-D-galactan of low molar mass (SAP2, 8.7 kDa). The results showed a high affinity of Salmonella for galactans, while high molar mass pectins showed no adhesion capacity. The chemical compositions of the fractions suggested that galactose could be responsible for the recognition process in the adhesion process. Other factors, such as structure and degree of polymerization of the polymers, may also be influencing the adhesion process. Modified soybean meal polysaccharides appear to be a promising alternative agent to antibiotics for the control and prevention of foodborne diseases.