Citrus Mealybug Performance and Plant Strata Preference on Different Coffee Varieties.

The optimal defense theory stipulates that a plant prioritizes the defense of young tissue against herbivory, which may affect the spatial distribution of the attacking insect and its impact on plant performance. In this study, we evaluated the feeding and oviposition site preferences of the citrus mealybug, Planococcus citri (Risso, 1813) (Hemiptera: Pseudococcidae), when comparing different parts of the canopy of two coffee varieties as well as its fertility and life history parameters. We evaluated the feeding preference, oviposition site choice, and the distribution of different development stages of P. citri on different strata (apical, median, base, and trunk regions) of two coffee varieties, Coffea arabica and Coffea canephora, as well as the associated variations in life history parameters and fertility. The citrus mealybug preferred to feed and oviposit on young leaves despite the presence of high levels of defense compounds. On average, more than 38% of P. citri preferred the apical leaves of cv. Coffea arabica compared to the other parts of the plant. However, in cv. C. canephora, the proportion that preferred the apical leaves was greater than 55%. The net reproduction rate (R0) and the intrinsic rate of increase (rm) of P. citri reared on C. canephora were significantly higher than those seen on C. arabica. The generation time (T) of P. citri reared on C. arabica was significantly longer than that observed on C. canephora. The estimated population growth rate of mealybugs on C. canephora varieties over 15 generations was more than twice that of the population on C. arabica. The dispersion ability and choice of the most nutritious plant strata by nymphs and adults on coffee plants are fundamental to the fitness of these mealybugs. Mealybugs showed a distinct preference for C. canephora.

Biological performance and oviposition preference of tomato pinworm Tuta absoluta when offered a range of Solanaceous host plants.

The tomato pinworm Tuta absoluta (Lepidoptera: Gelechuidae) is native to South America and has now become the main tomato pest in Europe, Africa and Asia. The wide range of host plants attacked by this pest has been reported as one of the main reasons for the success of this important insect species. However, the information currently available on the biological performance of T. absoluta on Solanaceae has been obtained from a limited number of host species. The Solanaceae family is composed of thousands of species, many of which are potential hosts for T. absoluta. Our results showed that the highest oviposition rates occurred on cultivated tomato plants, potato and wild tomato. The lowest rates occurred on "gilo", "jurubeba", green pepper and pepper. The highest survival rates of the immature stages occurred on potato and the lowest on pepper, green pepper and "jurubeba". Female fertility, following infestation of the different plant species, was highest for insects that developed on tomato or potato and the lowest rates were seen on American black nightshade. The net reproductive rate and the intrinsic growth rate were highest on potato and tomato. Cluster analysis grouped tomato and potato as highly susceptible to attack, American black nightshade, juá, eggplant, gilo and wild tomato as moderately susceptible, whilst pepper, green pepper and jurubeba were categorized as resistant to T. absoluta. These results clearly demonstrate that the choice of solanaceous host plant species has a direct impact on the fitness parameters of the tomato pinworm as well as survival potential, dispersion and establishment at new sites. These results are important for the planning of integrated pest management strategies.

Detection of Colletotrichum gloeosporioides in native cashew species in Brazil.

The savanna cashew (Anacardium humile A. St. Hil.) nut is a native shrub species distributed in the savanna biome of Central Western Brazil. This species is similar to the cashew (Anacardium occidentale L.) nut, and its edible pseudo-fruits have been used for various purposes. However, A. humile is at risk of extinction. One reason for this is the phytosanitary issues. Therefore, phytosanitary studies of the serious plant diseases caused by fungal pathogens are necessary. The aim of this study was to survey the savanna cashew nut (Anacardium humile) in Central Western Brazil from August 2013 to October 2014. Serious damage caused by anthracnose to the plant's leaves and fruits was consistently detected. The pathogen fungal species was Colletotrichum gloeosporioides. It was identified by morphological characteristics and molecular analyses. Additional experiments showed evidence that C. gloeosporioides can be transmitted by cashew seeds. We observed anthracnose symptoms on the surfaces of the floral peduncle, leaves, and peduncle of the A. humile cashew nuts. The genomic DNA sequences of the selected strains for molecular characterization have had 99% identity with the analogous sequences of C. gloeosporioides. Naturally occurring C. gloeosporioides infection of the leaves of A. humile was low (20.3%) compared with that of the nuts (79.7%). Our results can be used to design strategies to prevent the introduction and establishment of C. gloeosporioides in new areas. They can also be helpful in monitoring programs in areas with a current occurrence of C. gloeosporioides. Finally, these results can be used in future research plans of C. gloeosporioides infection management.

Negative cross-resistance between structurally different Bacillus thuringiensis toxins may favor resistance management of soybean looper in transgenic Bt cultivars.

High adoption rates of single-gene Bacillus thuringiensis (Bt) Cry1Ac soybean impose selection pressure for resistance in the soybean looper, Chrysodeixis includens, a major defoliator in soybean and cotton crops. To anticipate and characterize resistance profiles that can evolve, soybean looper larvae collected from field crops in Brazil in 2013 were selected for resistance to Cry1Ac. Using two methods of selection viz., chronic exposure to Cry1Ac cotton leaves and the seven-day larval exposure to purified Cry1Ac on the artificial diet, 31 and 127-fold resistance was obtained in 11 and 6 generations of selection, respectively. The resistance trait had realized heritability of 0.66 and 0.72, respectively, indicating that most of the phenotypic variation in Cry1Ac susceptibility of the soybean looper larvae was due to additive genetic variation. The Cry1Ac-selected populations showed positive cross-resistance to Cry1Ab (6.7-8.7 fold), likely because these Bt toxins have a very similar molecular structure. Importantly, the Cry1Ac-selected populations became more susceptible to Cry2Aa and Cry1Fa, showing negative cross-resistance (up to 6-fold, P < 0.05). These results indicate that Cry1Ac, Cry1Fa, and Cry2A are compatible in a multi-toxin approach to minimize the risk of rapid adaptation of the soybean looper to Bt toxins.

Spatial distribution of beetle attack and its association with mango sudden decline: an investigation using geostatistical tools.

BACKGROUND: Ceratocystis fimbriata recognized among the species that induce mango sudden decline (MSD), causes plant death within a short period. The beetles Hypocryphalus mangiferae and Xyleborus affinis (Curculionidae: Scolytinae) are the vectors of MSD. Thorough understanding of the spatial distribution of the pest is crucial to designing control techniques and drawing up sampling plans. This study aimed to identify the beetles and their dispersal pattern in mango trees in MSD-infected commercial orchards, and the association with the severity of the C. fimbriata infestation. RESULTS: Beetle attacks were observed to be maximal on mango tree trunks revealing severe infestation. From the geostatistical analysis, an aggregated pattern was evident as galleries in the trunks and branches of mango trees. CONCLUSION: This is the first study to employ geostatic tools on a plant scale in MSD-infested mango orchards and to study the incidence of beetle attack. The results may prove a highly effective tool for mango growers, with respect to the management of beetles and MSD, as this will facilitate the monitoring of specific sites where the frequency of beetles and MSD is high. © 2018 Society of Chemical Industry.

Yield Losses in Transgenic Cry1Ab and Non-Bt Corn as Assessed Using a Crop-Life-Table Approach.

In this study, we constructed crop life tables for Bacillus thuringiensis Berliner (Bt) Cry1Ab and non-Bt corn hybrids, in which yield-loss factors and abundance of predaceous arthropods were recorded during 2 yr at two locations. Corn kernel/grain was the yield component that had the heaviest losses and that determined the overall yield loss in the corn hybrids across years and locations. Yield losses in both corn hybrids were primarily caused by kernel-destroying insects. Helicoverpa zea (Boddie) and Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) were the key loss factors at one location, while at the other, the key loss factor was the silk fly larvae, Euxesta spp. (Diptera: Ulidiidae). Although the realized yield of corn grains was not different (P > 0.05) between Cry1Ab and non-Bt corn hybrids, the Bt corn hybrid reduced (P < 0.05) the damage by H. zea and S. frugiperda in three of the four field trials, particularly at the location where Lepidoptera were the key loss factors. As expected, no reduction in the abundance of predaceous arthropods was observed in Cry1Ab corn fields. Various species of natural enemies were recorded, particularly the earwig Doru luteipes (Scudder) (Dermaptera: Forficulidae), which was the most abundant and frequent predaceous insect. These results indicate that integration of pest management practices should be pursued to effectively minimize losses by kernel-destroying insects during corn reproductive stages when growing non-Bt or certain low-dose Bt corn cultivars for fall armyworm and corn earworm, such as those producing Cry1Ab or other Cry toxins.

Sampling Plans for the Thrips Frankliniella schultzei (Thysanoptera: Thripidae) in Three Lettuce Varieties.

The common blossom thrips, Frankliniella schultzei Trybom (Thysanoptera: Thripidae), is an important lettuce pest worldwide. Conventional sampling plans are the first step in implementing decision-making systems into integrated pest management programs. However, this tool is not available for F. schultzei infesting lettuce crops. Thus, the objective of this work was to develop a conventional sampling plan for F. schultzei in lettuce crops. Two sampling techniques (direct counting and leaf beating on a white plastic tray) were compared in crisphead, looseleaf, and Boston lettuce varieties before and during head formation. The frequency distributions of F. schultzei densities in lettuce crops were assessed, and the number of samples required to compose the sampling plan was determined. Leaf beating on a white plastic tray was the best sampling technique. F. schultzei densities obtained with this technique were fitted to the negative binomial distribution with a common aggregation parameter (common K = 0.3143). The developed sampling plan is composed of 91 samples per field and presents low errors in its estimates (up to 20%), fast execution time (up to 47 min), and low cost (up to US $1.67 per sampling area). This sampling plan can be used as a tool for integrated pest management in lettuce crops, assisting with reliable decision making in different lettuce varieties before and during head formation.