Mineral and potentially toxic element profiles in the soil-feed-animal continuum: Implications for public, environmental, and livestock health in three pasture-based sheep farming systems.

Grazing livestock derive most of their mineral requirements from foraging. The presence of toxic elements in soils has become a significant concern for food safety and ecosystem services. Understanding the mineral content profiles in soil and forage is crucial for assessing animal health, predicting potential transfers of minerals or heavy metals into the food-chain, and assessing threats to the environment and human health. In this study, Na, Mg, P, K, Ca, Mn, Fe, Co, Cu, Zn, Se, As, Cd, Hg, and Pb were measured to determine the mineral status of three different pasture-based farming systems (with grazing sheep livestock) in a Spanish region of significant economic importance. A risk assessment evaluation of animal, environmental, and human health was performed on soil, forage, feed, serum, milk, and wool. Notably, traces of Pb, and As were identified in pastures in all farms. Our calculation of pollution indices revealed moderate levels of contamination by various elements, including Co, Cu, Zn, Se, As, Cd, Hg, and Pb. The two farms with more intense agrosystem practices showed a significant potential ecological risk, characterized by high soil levels of Hg and Cd. Animals from these farms also had high concentrations of these metals in wool. Although the target Hazard Quotient derived from milk consumption suggests that dairy products from this area are safe for consumption for adults, only milk from a dehesa farm (mix of woodland and pastureland) was free of potential health concerns related to Pb exposure. Our assessment of mineral profiles reveals a cohesive relationship between soil quality and derived animal products, particularly of the Merino sheep breeding and farming system. The results reveal the importance of adopting and reinforcing strategies to preserve dehesas as a sustainable and environmentally friendly agrosystem in the western Mediterranean region.

Metal and metalloid concentrations in wild mammals from SW Europe: European hedgehog (Erinaceus europaeus) and badger (Meles meles).

In recent years, there have been increasing ecological and global concerns associated to Potentially Toxic Elements (PTEs). Thus, the relevance of wild mammals as biomonitors has been globally recognised. In the present study, Cd, Pb, Hg, Zn and As concentrations were quantified in European hedgehog and badger inhabiting SW Europe, and cumulative trends in relation to age and sex were evaluated. Liver and kidney samples were collected, mineralised and PTE content was determined by ICP-MS. Zn was the most abundant element quantified in both organs (239 and 89.8 mg kg-1 for hedgehogs and 179 and 164 mg kg-1 dw for badgers). In hedgehogs, very high Hg concentration were quantified (4.35 and 15.5 mg kg-1 dw in liver and kidney), and Cd was the most abundant for badgers (4.70 and 7.61 mg kg-1 dw in liver and kidney). Positive correlations were observed for the concentrations of PTE in the organs of both species. Age-dependence increased only Cd concentration, with levels in adult kidneys being significantly higher. In this study, European hedgehog and badger were used as biomonitors for the determination of PTEs to provide current reference values in relatively non-polluted areas of SW Europe, and to enhance the use of these species for future ecotoxicological studies.

Prevalence of burnout syndrome among veterinarians in Spain.

OBJECTIVE: Burnout is a syndrome of emotional distress common in health-care professions. The aim of this study was to assess the potential levels of burnout syndrome among veterinarians in Spain as well as different factors involved in its prevalence. SAMPLE: 282 Spanish veterinarians (57.4% women and 42.6% men). PROCEDURES: All data were obtained through an online survey. The instrument utilized for this purpose was the Maslach Burnout Inventory Human Services Survey (adapted to a Spanish-speaking population), with analyses performed using statistical software. Moreover, a sociodemographic questionnaire was utilized. RESULTS: Within the considered population, 23% suffered from burnout syndrome and the average scores were 29 points for emotional exhaustion, 14 points for depersonalization, and 35 points for personal accomplishment. A higher prevalence of burnout was associated with small animal clinicians (75.3%) and women (66.1%), in comparison to large animal clinicians (24.6%) and men (33.8%). CLINICAL RELEVANCE: This study showed for the first time that burnout syndrome is a current and highly relevant risk for Spanish veterinarians. The results obtained could help to raise awareness of this reality and the factors involved in the development or exacerbation of this syndrome.

Effects of carbofuran and deltamethrin on acetylcholinesterase activity in brain and muscle of the common carp.

This work investigated the effect from exposure to insecticides carbofuran and deltamethrin on acetylcholinesterase (AChE) activity in the brain and muscle of common carp (Cyprinus carpio). Both pesticides were evaluated through two separate experiments, and carp were exposed in a semi-static system to three different concentrations of carbofuran (10, 50, and 100 µg/L) and deltamethrin (0.08, 0.4, and 0.8 µg/L) during a month with sampling times at 0, 4, 15, and 30 days (n = 7 from each aquarium). AChE activity was significantly inhibited in both organs of carps exposed to carbofuran at all sampling times depending on dose and time, reaching inhibition values of 73.5 and 67.1%, in brain and muscle tissues respectively, after 30 days with the highest concentration. On the contrary, AChE activity was not significantly affected after deltamethrin exposure at all concentrations and times of the assay. This study shows that the measurement of brain and muscle AChE activity in Cyprinus carpio is a useful biomarker of carbamates exposure and/or effects, but has no application with pyrethroids.

Body feathers as a potential new biomonitoring tool in raptors: a study on organohalogenated contaminants in different feather types and preen oil of West Greenland white-tailed eagles (Haliaeetus albicilla).

We investigated the variation in concentrations and profiles of various classes of organohalogenated compounds (OHCs) in different feather types, muscle tissue and preen oil from 15 white-tailed eagle (Haliaeetus albicilla) carcasses from Greenland. The influence of moult patterns and potential external contamination onto the feather surface was examined, while the present study is also the first to investigate the use of body feathers for OHC monitoring. Concentrations of sum polychlorinated biphenyls (PCBs) in feathers from white tailed eagles ranged from 2.3 ng/g in a primary wing feather to 4200 ng/g in body feathers. Using 300 mg of body feathers, almost 50 different OHCs could be quantified and median concentrations in body feathers were 10 fold higher than concentrations in tail feathers (rectrices) or primary wing feathers. Body feathers could be very useful for biomonitoring taking into account their easy sampling, short preparation time and high levels of OHCs. In addition, the effects of confounding variables such as feather size, moult and age are also minimised using body feathers. Correlations with concentrations in muscle tissue and preen oil were high and significant for all feather types (r ranging from 0.81 to 0.87 for sum PCBs). Significant differences in concentrations and profiles of OHCs were found between different primary feathers, indicating that the accumulation of OHCs in feathers varies over the moulting period (maximum three years). Washing of feathers with an organic solvent (acetone) resulted in a significant decrease in the measured concentrations of OHCs in feathers. However, our results indicated that preen oil is probably not the only contributor to the external contamination that can be removed by washing with acetone. Possibly dust and other particles may be of importance and may be sticking to the preened feathers. Rectrices washed only with water showed high and significant correlations with concentrations in muscle and preen oil as well. Washing with acetone therefore does not seem to be of great influence when relating to internal tissue concentrations. We recommend washing feathers only with distilled water in order to remove dirt and dust particles before analysis.