ABSTRACT
Two strains of an unknown Gram-positive, catalase-negative, facultatively anaerobic coccus originating from the reproductive tract of horses were characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the two strains constitute a new subline within the lactic-acid group of bacteria, close to, but distinct from, Abiotrophia defectiva, Globicatella sanguinis and close relatives. The unknown bacterium was readily distinguished from other described Gram-positive, catalase-negative cocci by biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Eremococcus coleocola gen. nov., sp. nov. The type strain of Eremococcus coleocola is CCUG 38207T.
Subject(s)
Gram-Positive Bacterial Infections/veterinary , Gram-Positive Cocci/classification , Gram-Positive Cocci/genetics , Horse Diseases/microbiology , Vaginal Discharge/veterinary , Animals , Bacterial Typing Techniques , Catalase/metabolism , Female , Genes, rRNA , Gram-Positive Bacterial Infections/microbiology , Gram-Positive Cocci/isolation & purification , Horses , Molecular Sequence Data , Phylogeny , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Vaginal Discharge/microbiologyABSTRACT
Phenotypic and phylogenetic studies were performed on a hitherto undescribed Gram-positive, catalase-negative, chain-forming coccus isolated from human blood. Comparative 16S rRNA gene sequencing studies demonstrated that the unknown organism constitutes a new phylogenetic line, close to, but distinct from, Facklamia and Globicatella. The unknown bacterium was readily distinguished from currently recognized Facklamia species and Globicatella sanguinis by biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Dolosicoccus paucivorans gen. nov., sp. nov. The type strain of Dolosicoccus paucivorans is CCUG 39307T.
Subject(s)
Blood/microbiology , Gram-Positive Bacterial Infections/microbiology , Gram-Positive Cocci/classification , Gram-Positive Cocci/genetics , Bacteremia/microbiology , Bacterial Typing Techniques , Catalase/metabolism , Genes, rRNA , Gram-Positive Cocci/isolation & purification , Gram-Positive Cocci/physiology , Humans , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction/methods , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNAABSTRACT
A hitherto unknown Gram-positive, catalase-negative, facultatively anaerobic coccus isolated from a vesicle on the gum of a dog was characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the isolate represents a new subline within the genus Gemella. The unknown bacterium was readily distinguished from all currently described members of this genus, Gemella haemolysans, Gemella bergeri, Gemella morbillorum and Gemella sanguinis, by biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Gemella palaticanis sp. nov. The type strain of Gemella palaticanis is CCUG 39489T.
Subject(s)
Dog Diseases/microbiology , Gram-Positive Bacterial Infections/veterinary , Gram-Positive Cocci/classification , Mouth/microbiology , Animals , Bacterial Typing Techniques , Dogs , Genes, rRNA , Gram-Positive Bacterial Infections/microbiology , Gram-Positive Cocci/isolation & purification , Gram-Positive Cocci/physiology , Male , Molecular Sequence Data , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNAABSTRACT
Phenotypic and phylogenetic studies were performed on a hitherto undescribed micro-organism isolated from the human vagina. Comparative 16S rRNA gene sequencing studies demonstrated that the unknown strain constituted a new subline within the genus Atopobium. The unknown bacterium was readily distinguished from other Atopobium species by biochemical tests and electrophoretic analysis of whole-cell proteins. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Atopobium vaginae sp. nov. The type strain of Atopobium vaginae is CCUG 38953T.
Subject(s)
Actinobacteria/classification , Actinobacteria/genetics , Vagina/microbiology , Actinobacteria/isolation & purification , Actinobacteria/physiology , Bacterial Typing Techniques , Female , Genes, rRNA , Humans , Molecular Sequence Data , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNAABSTRACT
The gene organization and nucleotide sequence of the type A and B BoNT-gene clusters in Clostridium botulinum strain NCTC 2916 were studied. The aim was to clarify the organization of genes within C. botulinum type A strains possessing an unexpressed BoNT/B gene. The BoNT/A-gene cluster includes genes encoding BoNT, NTNH and a part of P-47 (the gene for this protein was reported in strains of C. botulinum types E and F). Clustered with the silent BoNT/B gene were genes encoding NTNH, P-21 and HA-33. Sequencing analysis of the NTNHs revealed the presence of 471 amino acids identical in the type B and A gene clusters. This gene organization contrasts markedly with the purported organization in strain NCTC 2916 described by Henderson et al. (FEMS Microbiol. Lett. 140, 151-158). In type A(B) strain NCTC 2916, the neurotoxin gene is of type BoNT/A1 within a gene cluster that has identical organization to that found in BoNT/A2 type strains; these observations may be significant in establishing the origin of the BoNT-gene cluster.
