ABSTRACT
The immunohistological findings using antibodies to different intermediate filaments (glial fibrillary acidic protein, vimentin and two types of cytokeratin) and epithelial membrane antigen are described in 89 gliomas, 19 meningiomas and 8 choroid plexus papillomas (CPPs) from adult patients. All the patients had total or subtotal surgical excision of their tumours with clinical follow up for between 3 and 7 years. The immunohistological results were correlated with the histological features and patient survival. Tumours other than low grade astrocytomas, oligodendrogliomas and anaplastic ependymomas expressed one or more epithelial markers. This immunohistological evidence of epithelial differentiation in the absence of histological epithelial features in gliomas confirms that the two are not necessarily correlated. It is concluded that the expression of epithelial markers in some intradural tumours may reflect aberrant differentiation related to the degree of anaplasia in poorly differentiated astrocytomas and glioblastomas. All the patients with anaplastic epithelial marker-positive gliomas died within 1 year, whereas only 68% of patients with marker-negative tumours died within the follow-up period. In ependymomas and meningiomas, the expression of epithelial markers may reflect their histogenesis, while in malignant CPPs such expression could denote either their aberrant differentiation or histogenetic derivation.
Subject(s)
Biomarkers, Tumor/analysis , Brain Neoplasms/pathology , Choroid Plexus Neoplasms/pathology , Glioma/pathology , Meningioma/pathology , Neoplasm Proteins/analysis , Nerve Tissue Proteins/analysis , Papilloma/pathology , Adolescent , Adult , Aged , Brain Neoplasms/chemistry , Brain Neoplasms/mortality , Cell Differentiation , Choroid Plexus Neoplasms/chemistry , Choroid Plexus Neoplasms/mortality , Ependymoma/chemistry , Ependymoma/mortality , Ependymoma/pathology , Epithelium/chemistry , Epithelium/pathology , Female , Glioma/chemistry , Glioma/mortality , Humans , Intermediate Filament Proteins/analysis , Male , Meningioma/chemistry , Meningioma/mortality , Middle Aged , Papilloma/chemistry , Papilloma/mortalityABSTRACT
25 neuronal tumours with a panel of antibodies were studied and it was found that vimentin was present in 15 tumours. It was also found in a few cells within rosettes. PGP 9.5 showed a somatic pattern of staining with nuclear and perinuclear positivity in 23. Neurofilament reactivity was found in 14. Retinal S-antigen was detected only in one medulloblastoma, 3/4 pineal tumours and 2/2 retinoblastomas. Reactivity, for synaptophysin was present in 2/5 medulloblastomas, 3/10 neuroblastomas and 2/2 retinoblastomas. GFAP was demonstrated in scattered tumour cells in 4/5 medulloblastomas. Two of these were the only tumours featuring bipolar differentiation whilst it was unipolar in the remainder. The significance of these findings in relation to the ontogeny of these tumours is discussed.
Subject(s)
Central Nervous System Neoplasms/chemistry , Neurofilament Proteins/analysis , Vimentin/analysis , Adolescent , Adult , Antibodies , Antibodies, Monoclonal , Brain Chemistry , Child , Child, Preschool , Glial Fibrillary Acidic Protein/analysis , Humans , Immunohistochemistry , Infant , Middle Aged , Neuropeptides/analysis , Retina/chemistry , Synaptophysin/analysis , Ubiquitin ThiolesteraseABSTRACT
Paraffin-embedded blocks of 36 cerebellar haemangioblastomas were reacted with a panel of antibodies including glial fibrillary acidic protein, vimentin, epithelial membrane antigen, cytokeratin, Factor VIII, a neuroendocrine marker and with Ulex europaeus. agglutinin The main histological features, apart from the characteristic large abnormal vessels, were a prominent reticulin network, a cystic architecture and cellular and nuclear polymorphism. Two cell types were identified: endothelial and stromal. Twenty tumours were positive for glial fibrillary acidic protein because of included or reactive astrocytes as well as positive stromal cells. Vimentin was positive in all tumours with a diffuse distribution and a somatic pattern; blood vessels, stromal cells and reactive astrocytes were strongly positive. Factor VIII and Ulex europaeus agglutinin reactivity were present in a similar pattern of staining in endothelium and in five cases there were stromal cells that were positive with the latter. We were not able to ascertain the histogenesis of the stromal cell, which remains enigmatic.
Subject(s)
Cerebellar Neoplasms/pathology , Hemangiosarcoma/pathology , Plant Lectins , Adolescent , Adult , Aged , Cerebellar Neoplasms/metabolism , Child , Factor VIII/metabolism , Female , Glial Fibrillary Acidic Protein/metabolism , Hemangiosarcoma/metabolism , Humans , Immunohistochemistry , Lectins , Male , Middle Aged , Vimentin/metabolismABSTRACT
Cystinosis is frequently presented with cystine storage in the cornea and conjunctiva, and the diagnosis can be established by slit-lamp examination. It can also be confirmed by electron microscopy of a conjunctival biopsy. The present paper reports on a 16-month-old boy with Fanconi's syndrome, in whom the slit-lamp examination did not show crystal deposits of cystine in the conjunctiva. The ultrastructural study of the conjunctival biopsy demonstrated polygonal crystals within double membrane-limited organelles located in fibroblasts. Similar crystals were subsequently found in a kidney biopsy. We therefore think that conjunctival biopsy is a valuable diagnostic tool prior to performing renal biopsy, even in cases with negative findings by ophthalmologic examination.
Subject(s)
Conjunctiva/pathology , Cystinosis/pathology , Cystinosis/therapy , Fanconi Syndrome/complications , Fanconi Syndrome/pathology , Humans , Infant , Kidney/pathology , Lysosomes/ultrastructure , Male , Microscopy, ElectronABSTRACT
La biopsia del endometrio en la paciente estéril necesita de nuevos aportes para una mejor interpretación de los hallazgos clínicos y morfológicos. Esta investigación se ocupa de la aplicación de la técnica de Mowry para determinar prototipos histoquímicos en los endometrios proliferativos de las pacientes que consultaron por esterilidad. Se estudiaron retrospectivamente 52 biopsias provenientes de igual número de pacientes atendidas en distintos consultorios de ginecología de la fertilidad; se usaron como testigos, 47 endometrios provenientes de histerectomías simples. Se encontraron 4 prototipos de comportamiento estromal: Prototipo I (negatio total) o del endometrio del post aborto; el prototipo II (difuso y perivascular leve) del proliferativo ciclico temprano; el prototipo III (positivo difuso y perivascular intenso) del proliferativo ciclico medio y del monofásico y el prototipo IV (positivo focal leve o moderado) del proliferativo ciclico tardío. Se concluye que la técnica de Mowry aplicada a los endometrios proliferativos ciclicos y no ciclicos es un recurso complementario aplicable al material biópsico de la paciente que consulta por esterilidad
Subject(s)
Humans , Female , Biopsy , Endometrium/pathology , Glycosaminoglycans/analysis , Infertility, FemaleABSTRACT
La biopsia del endometrio en la paciente estéril necesita de nuevos aportes para una mejor interpretación de los hallazgos clínicos y morfológicos. Esta investigación se ocupa de la aplicación de la técnica de Mowry para determinar prototipos histoquímicos en los endometrios proliferativos de las pacientes que consultaron por esterilidad. Se estudiaron retrospectivamente 52 biopsias provenientes de igual número de pacientes atendidas en distintos consultorios de ginecología de la fertilidad; se usaron como testigos, 47 endometrios provenientes de histerectomías simples. Se encontraron 4 prototipos de comportamiento estromal: Prototipo I (negatio total) o del endometrio del post aborto; el prototipo II (difuso y perivascular leve) del proliferativo ciclico temprano; el prototipo III (positivo difuso y perivascular intenso) del proliferativo ciclico medio y del monofásico y el prototipo IV (positivo focal leve o moderado) del proliferativo ciclico tardío. Se concluye que la técnica de Mowry aplicada a los endometrios proliferativos ciclicos y no ciclicos es un recurso complementario aplicable al material biópsico de la paciente que consulta por esterilidad (AU)
