ABSTRACT
The article deals with a comparative analysis of the parameters of the polymerization in physiological conditions of three commercially available alkyl cyanoacrylates, n-butyl cyanoacrylate (GLUBRAN 2), n-hexyl cyanoacrylate (IFABOND), and n-octyl cyanoacrylate (EVOBOND), the cell behavior of the corresponding polymers and the application of these adhesives in the fixation of surgical polypropylene meshes for hernia repair in an animal model of rabbits. The results obtained demonstrate that the curing process depends on the nature of the alkyl residue of the ester group of cyanoacrylate molecules, being the heat of polymerization lower for the octyl derivative in comparison with the hexyl and butyl, and reaching a maximum temperature of 35 °C after a time of mixing with physiological fluids of 60-70 s. The cell behavior demonstrates that the three systems do not present toxicity for fibroblasts and low adhesion of cells, which is a positive result for application as tissue adhesives, especially for the fixation of abdominal polypropylene meshes for hernia repair. The animal experimentation indicates the excellent tolerance of the meshes fixed with the cyanoacrylic adhesives, during at least a period of 90 d, and guarantees a good adhesion for the application of hernia repair meshes.
Subject(s)
Cyanoacrylates/chemistry , Hernia, Abdominal/surgery , Herniorrhaphy/methods , Surgical Mesh , Tissue Adhesives/pharmacology , Animals , Biomechanical Phenomena , Cell Adhesion/drug effects , Cell Proliferation/drug effects , Cells, Cultured , Cyanoacrylates/administration & dosage , Fibroblasts/cytology , Fibroblasts/drug effects , Humans , Macrophages/cytology , Macrophages/drug effects , Male , Models, Animal , Polymerization , Polypropylenes/chemistry , Prosthesis Design , Rabbits , Skin/cytology , Skin/drug effects , Tissue Adhesives/chemistryABSTRACT
Introducción. En algunas enfermedades de origen peritoneal, para evitar la aparición de un síndrome compartimental debe realizarse el cierre temporal de la cavidad abdominal. Así, es posible mantener la presión intraabdominal normal y preservar los bordes fasciales para un cierre definitivo posterior. Material y método. Se crearon defectos de 7 * 4 cm en la pared anterior del abdomen de conejos blancos de Nueva Zelanda, que fueron sustituidos por una lámina ovalada de una prótesis de diseño propio (PL-PU99) y una de origen biológico (Surgisis®) de las mismas dimensiones. El biomaterial fue fijado a los bordes de la pared mediante dos suturas continuas de polipropileno interrumpidas únicamente en los ángulos, dejando la prótesis en contacto con el exterior. A los 14 días postimplante se tomaron muestras para estudios de microscopia óptica y electrónica de barrido, morfométricos e inmunohistoquímicos (RAM-11). Resultados. No hubo mortalidad ni presencia de rechazo en los implantes. Se observaron pequeñasáreas de adherencia de tipo laxo en algunos implantes; el porcentaje que ocupaban era de 0,31 ± 0,03% para el PL-PU99 y 31,60 ± 7,35% para el Surgisis®. El neoperitoneo era homogéneo y bien organizado en los dos tipos de prótesis, con un espesor de 427,60 ± 8,38 µm (PL-PU99) y 171,99 ± 18,70 µm (Surgisis®). No se observaron diferencias significativas en cuanto a la reacción macrofágica (PL-PU99 19,76 ± 1,59%; Surgisis® 21,07 ± 8,93%). Conclusiones. a) La prótesis PL-PU99 genera una menor formación adherencial y un mayor espesor del neoperitoneo, y b) ambas prótesis probablemente podrían ser aptas para ser empleadas en clínica en el cierre temporal de la cavidad abdominal (AU)
Introduction. In some disease of peritoneal origin, temporary closure of the abdominal cavity is required to avoid compartmental syndrome. This allows normal intra-abdominal pressure to be maintained and the fascial edges to be preserved for subsequent definitive closure. Material and method. Defects ( 7x 4 cm) were created in the anterior abdominal wall of New Zealand white rabbits and were repaired using an oval-shaped patch of a prosthesis designed of biological origin Surgisis® of similar dimensions to the defects. The biomaterials were fixed to the cut edges of the wall by 2 polypropylene running sutures interrupted only at the corners, leaving the patch in contact with the atmosphere. Fourteen days after implantation, prosthesis/anchorage tissue specimens were taken for light and scanning electron microscopy, morphometric measurements and immunohistochemical macrophage identification (Using the RAM-11 antibody). Results. There were no cases of mortality or implant rejection. Small areas of loose adhesions were observed on some implants (covering 0.31 +/- 0.03% of the PL-PU99 implants and 31.60 +/- 7.35% of Surgisis®). The neoperitoneum induced by both implant types was homogenous and well organized, with thicknesses of 427.60 +/- 8.38 mm (PL-PU99) and 171.99 +/- 18.70 mm (Surgisis®). No significant differences were observed in terms of the macrophage reaction induced (PL-PU99 19.76 +/-1.59%; Surgisis® 21.07 +/- 8.93% macrophages). Conclusions. A) The PL-PU99 prosthesis provoked fewer adhesions and generated a thicker neoperitoneum. B) Both prostheses would probably be suitable for temporary closure of the abdominal cavity (AU)
Subject(s)
Rabbits , Animals , Rabbits , Prosthesis Implantation/methods , Abdomen/surgery , Biocompatible Materials/analysis , Compartment Syndromes/prevention & control , Animal Experimentation , Digestive System Surgical Procedures/methods , Polypropylenes/therapeutic use , Polyurethanes/therapeutic use , Prosthesis Design/methodsABSTRACT
INTRODUCTION: In some diseases of peritoneal origin, temporary closure of the abdominal cavity is required to avoid compartmental syndrome. This allows normal intra-abdominal pressure to be maintained and the fascial edges to be preserved for subsequent definitive closure. MATERIAL AND METHOD: Defects (7 x 4 cm) were created in the anterior abdominal wall of New Zealand white rabbits and were repaired using an oval-shaped patch of a prosthesis designed by our team (PL-PU99) or a prosthesis of biological origin (Surgisis) of similar dimensions to the defects. The biomaterials were fixed to the cut edges of the wall by 2 polypropylene running sutures interrupted only at the corners, leaving the patch in contact with the atmosphere. Fourteen days after implantation, prosthesis/anchorage tissue specimens were taken for light and scanning electron microscopy, morphometric measurements and immunohistochemical macrophage identification (using the RAM-11 antibody). RESULTS: There were no cases of mortality or implant rejection. Small areas of loose adhesions were observed on some implants (covering 0.31 +/- 0.03% of the PL-PU99 implants and 31.60 +/- 7.35% of Surgisis). The neoperitoneum induced by both implant types was homogenous and well organized, with thicknesses of 427.60 +/- 8.38 microm (PL-PU99) and 171.99 +/- 18.70 microm (Surgisis). No significant differences were observed in terms of the macrophage reaction induced (PL-PU99 19.76 +/- 1.59%; Surgisis 21.07 +/- 8.93% macrophages). CONCLUSIONS: a) The PL-PU99 prosthesis provoked fewer adhesions and generated a thicker neoperitoneum. b) Both prostheses would probably be suitable for temporary closure of the abdominal cavity.
