ABSTRACT
A novel actinomycete, strain HMC10(T), was isolated from a soil sample taken from the banks of the Gamka River in the Swartberg Nature Reserve, Western Cape Province, South Africa. Strain HMC10(T) was identified as a member of the genus Nonomuraea by a polyphasic approach. Strain HMC10(T) could be differentiated from other members of the genus Nonomuraea on the basis of physiology and 16S rRNA gene sequence analysis. DNA-DNA hybridization further differentiated strain HMC10(T) from its nearest phylogenetic neighbour, Nonomuraea turkmeniaca NRRL B-16246(T) (4.5 +/- 3.8% DNA relatedness). Strain HMC10(T) exhibited weak antibiosis against Mycobacterium aurum A+, but none against Mycobacterium tuberculosis H37Rv(T). The name Nonomuraea candida is proposed, with the type strain HMC10(T) (= DSM 45086(T) = NRRL B-24552(T)).
Subject(s)
Actinomycetales/genetics , RNA, Ribosomal, 16S/genetics , Soil Microbiology , Actinomycetales/classification , Actinomycetales/ultrastructure , Microscopy, Electron, Scanning , Phylogeny , Sequence Analysis, DNA , South AfricaABSTRACT
Two novel nocardioform actinomycetes, strains Q41T and HMC25T, were isolated from soil samples collected in the Western Cape province, South Africa. Rapid genus identification revealed that the isolates belonged to the genus Kribbella (based on single-digestion restriction analysis of the 16S rRNA gene sequences with MboI, VspI, SphI, SnaBI, SalI and AgeI). Both isolates had ll-diaminopimelic acid and glycine in their cell-wall peptidoglycan, and contained mannose and ribose as whole-cell sugars. Strain HMC25T is able to grow at 45 degrees C and in the presence of NaCl (3 %), cephaloridine (10 microg ml(-1)) and gentamicin sulphate (10 microg ml(-1)). Strain Q41T grows in the presence of NaCl (2 %). Neither strain was able to grow under anaerobic conditions, whereas Kribbella flavida KACC 20248T, Kribbella jejuensis HD9T, Kribbella koreensis KACC 20250T and Kribbella sandramycini KACC 20249T exhibited weak but distinct growth under anaerobic conditions. Physiological test results and 16S rRNA gene sequence analysis allowed Q41T and HMC25T to be distinguished from other members of the genus with validly published names. Strains HMC25T (=NRRL B-24426T=DSM 17345T) and Q41T (=NRRL B-24425T=DSM 17344T) therefore represent the type strains of novel species, for which the names Kribbella swartbergensis sp. nov. and Kribbella karoonensis sp. nov., respectively, are proposed.
Subject(s)
Actinomycetales/classification , Actinomycetales/isolation & purification , Soil Microbiology , Actinomycetales/chemistry , Actinomycetales/physiology , Anaerobiosis , Anti-Bacterial Agents/pharmacology , Bacterial Typing Techniques , Carbohydrates/analysis , DNA Fingerprinting , DNA Restriction Enzymes/metabolism , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Bacterial/metabolism , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Diaminopimelic Acid/analysis , Genes, rRNA , Glycine/analysis , Growth Inhibitors/pharmacology , Molecular Sequence Data , Peptidoglycan/chemistry , Phylogeny , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Sodium Chloride/pharmacology , South AfricaABSTRACT
An actinomycete, strain B60(T), was isolated from a soil sample in Napier, Western Cape province of South Africa. Based on 16S rRNA gene sequence analysis and chemotaxonomy, strain B60(T) was identified as a member of the genus Actinomadura. Strain B60(T) produced an antibiotic with activity against Escherichia coli, Enterococcus faecium and Mycobacterium aurum, but not against Mycobacterium tuberculosis. Significant differences in morphological and physiological characteristics indicate that strain B60(T) represents a novel species of the genus Actinomadura. The name Actinomadura napierensis sp. nov. is proposed. The type strain is B60(T) (=DSM 44846(T)=NRRL B-24319(T)).
