Acute toxicity and biomarker responses in Gammarus locusta amphipods exposed to copper, cadmium, and the organochlorine insecticide dieldrin.

The toxicity of copper, cadmium, and dieldrin in adult Gammarus locusta (a marine amphipod) is currently unclear. Thus, G. locusta from the North Lake of Tunis were subjected to acute toxicity tests to assess LC50s at 48-96 h and to biomarker response tests through the assessment of catalase and acetylcholinesterase activities and malondialdehyde levels. The present study demonstrated the abilities of a chlorinated hydrocarbon pesticide (dieldrin) induce to oxidative stress and neurotoxicity. The comparison of metal toxicity showed that G. locusta was more sensitive to cadmium than copper. The three stressors caused significant inductions of all three biomarkers in a concentration-dependent manner. Catalase induction was dependent on exposure duration for all pollutants, while only copper led to increased malondialdehyde with longer exposure times. Catalase induction and malondialdehyde increase appeared to be sex dependent for all three pollutants. The neurotoxic effects of the pollutants were concentration dependent according to inhibition of acetylcholinesterase activity. In conclusion, catalase, malondialdehyde, and acetylcholinesterase are efficient biomarkers of copper, cadmium, and dieldrin in G. locusta.

Meiobenthic nematode Oncholaimus campylocercoides as a model in laboratory studies: selection, culture, and fluorescence microscopy after exposure to phenanthrene and chrysene.

Numerous studies have focused on the response of meiofauna after exposure to polycyclic aromatic hydrocarbons (PAHs), but none has been devoted to their uptake into nematode body compartments. The present study monitored PAH uptake by Oncholaimus campylocercoides which was selected after 40 days in the laboratory through original protocols from natural sediments collected in the Old Harbor of Bizerte, Tunisia. To achieve the mono-species level, a grain size magnification was applied by gradually adding a biosubstrate made from either the crushed shells of Mytilus galloprovincialis or minced leaves of Posidonia oceanica. After selection, O. campylocercoides was cultured and fed with earthworm powder (560 mg.l-1). Thereafter, it was exposed for 3 weeks to phenanthrene and chrysene (38, 116, and 348 ppb). Fluorescence microscopy revealed higher intensities of PAHs at the spicules, mouths, and pharynges compared with the other organs considered. Moreover, the buccal fluorescence showed a significant correlation with that measured in the biosubstrate made with shells of M. galloprovincialis.

Nematode traits after separate and simultaneous exposure to Polycyclic Aromatic Hydrocarbons (anthracene, pyrene and benzo[a]pyrene) in closed and open microcosms.

The majority of experimental studies carried out to date, regarding the effects of pollutants on meiofauna have been conducted by means of closed systems, and rarely using open ones. The current work explored the impact of three Polycyclic Aromatic Hydrocarbons (PAHs), anthracene, pyrene and benzo[a]pyrene, applied alone or combined, on meiobenthic nematodes using both systems. The results revealed that single PAHs impacted the nematofauna similarly in closed or open systems with a higher toxicity observed for benzo[a]pyrene. However, the closed microcosms contaminated with PAHs became organically enriched, resulting in more non-selective deposit feeders and omnivores-carnivores. Taxonomic and functional effects related to combinations of PAHs were close to those of individual treatments in closed systems, however, for open ones, the outcomes were different. The caudal morphology influenced the response of taxa during their avoidance/endurance of hydrocarbons in open systems where the effects of PAHs mixtures appeared not only additive but also synergetic. Based on the results of the study, the use of open systems is preferred to closed ones as the research outcomes were more accurate and representing better conditions prevailing in nature.

Do presence of gray shrimp Crangon crangon larvae influence meiobenthic features? Assessment with a focus on traits of nematodes.

In this study, a microcosm experiment was conducted for 30 days to assess the impact of the presence of juvenile gray shrimp Crangon crangon on meiofauna. The results suggested that juvenile shrimp had a significant negative impact on the abundance of nematodes and copepods, but no effect on polychaetes. Moreover, nematodes showed a significant decline in individual weight. The collected nematodes were taxonomically identified and assigned to five functional traits: shapes of the tail and amphid, life history, feeding types, and adult length. The nematode traits were affected by the number of shrimp introduced, and descriptors followed normal or inversed bell-shaped curves. When no shrimp were present, the nematofauna had a higher species richness compared with treatments of 4, 8, and 12 shrimp. Bell-shaped curve patterns were common in relation to the two phases of feeding for C. crangon. During the first phase, C. crangon consumed the nematode species Oncholaimus campylocercoides; thereafter, shrimp fed mostly on the nematode Anticoma eberthi and copepods.

Effects of Au/TiO2 metallic nanoparticles on Unio ravoisieri: assessment through an oxidative stress and toxicity biomarkers.

Several studies have been performed on the effects of nanoparticles on aquatic life. However, most of them investigated marine organisms, not freshwater organisms. This study investigated biomarker responses after exposure for 48 h and 7 days to newly made gold and titanium dioxide (Au/TiO2) metallic nanoparticles (MNPs) (100 and 200 µg·L-1) using the freshwater bivalve mussel Unio ravoisieri. Biochemical analysis of the gills and digestive glands showed induction of oxidative stress following exposure of the bivalve to Au/TiO2 MNPs. After 2 or 7 days of exposure to Au/TiO2 MNPs, both utilized concentrations of Au/TiO2 MNPs induce an overproduction of H2O2. Catalase and glutathione S-transferase activities and the malonedialdehyde content significantly increased in the presence of Au/TiO2 MNPs, depending on the concentration and target organ. In contrast, acetylcholinesterase activity was significantly inhibited, indicating a discernible disturbance of the cholinergic system in the presence of Au/TiO2 MNPs. The behavior of the freshwater mussel was altered by reducing the clearance rate. Therefore, U. ravoisieri can be used as a model species in laboratory studies to mirror the presence of MNPs, and the biomarker approach is important for detecting the effects of Au/TiO2 MNPs. In addition, digestive gland is the target organ of Au/TiO2NPs contamination.