Comparison of DNA damages in blood lymphocytes of indoor swimming pool lifeguards with non-lifeguards athletes.

Chlorination has been used as a major disinfectant process for swimming pool water in many countries. The purpose of this study is to compare the DNA damage of the blood lymphocytes in indoor pool lifeguards with non-lifeguards athletes. We performed a study in which the participants were Gonabad's lifeguards. We chose 30 participants (15 male and 15 female) for each group. We collected vein blood samples from each participant in both exposed and control group. The lymphocytes were isolated from the whole blood by ficoll, and the cell viability was determined by the trypan blue. The alkaline Comet assay was also performed on lymphocytes in order to measure the DNA damage. All the parameters indicated that the DNA damage was significantly greater in lifeguards group than control group (p < 0.001). Also, the results revealed a statistically significant higher level of DNA damage in females as evident by an increase in the tail length (µm) [8.97 ± 4.21 for females as compared to 4.32 ± 1.33 for males (p = 0.001)], tail DNA (%) [4.18 ± 1.27 for females as compared to 3.14 ± 0.94 for males (p = 0.016)] and tail moment (µm) [0.68 ± 0.53 for females and 0.26 ± 0.14 for males (p = 0.010)]. There was also a significant positive correlation between DNA damage and the duration of work (P < 0.001).

Nutritional Education Needs in Relation to Ramadan Fasting and Its Complications in Tehran, Iran.

BACKGROUND: Ramadan fasting is associated with some lifestyle changes. A lack of nutritional needs knowledge or the improper performance of fasting, particularly in relation to time, type and amount of food intake, can cause disorders such as indigestion, bloating, constipation, headaches and other clinical problems. OBJECTIVES: To investigate the general knowledge regarding dietary factors associated with Ramadan fasting and its related complications. PATIENTS AND METHODS: This prospective, non-interventional, observational study was conducted from April to July, 2012 to coincide with the month before and the month of Ramadan. The initial participants were 600 fasting and 588 non-fasting people (aged 18 - 65 years, BMI 18.5 - 40 kg/m2) chosen by random cluster sampling in Tehran, Iran. A questionnaire of Ramadan fasting nutritional knowledge was developed and validated in a pilot study. The Likert scale was used two weeks before Ramadan and during the third and fourth weeks of Ramadan to estimate Ramadan-related complications. Seven-day, 24 - hour food recalls were used to assess food intakes. RESULTS: The lowest level of general knowledge was identified in the context of foods associated with hunger (22.1%) and hypoglycemia (24.8%) and the highest level of general knowledge was identified in reference to unsuitable foods for Sahar (91.4%). During Ramadan, all attributed complications increased in fasting subjects (P < 0.001). High calorie, carbohydrate, fat and protein intakes in the Ramadan diet were associated with some gastrointestinal and sleep complications (P < 0.05). CONCLUSIONS: Despite the relatively high level of knowledge in the context of the general principles of a diet to prevent Ramadan-related complications, practical training in regard to the amounts of nutrients associated with Ramadan-related complications is both necessary and recommended.

Ramadan major dietary patterns.

BACKGROUND: There has been no data on population based dietary patterns during the Ramadan fasting month. OBJECTIVES: The purpose of this study was to detect Ramadan major dietary patterns among those who fast in Tehran. MATERIALS AND METHODS: This cross-sectional study included 600 subjects, aged 18-65 with body mass index (BMI) of 18.5-40, who had decided to fast during Ramadan. Anthropometric measurements, usual physical activity level and educational status were collected two weeks before Ramadan. Information on Ramadan dietary intakes was obtained using a food frequency questionnaire and factor analysis was used to identify major dietary patterns. RESULTS: We identified four major dietary patterns: 1) Western-like pattern; high in fast foods, salty snacks, nuts, potato, fish, poultry, chocolates, juices; 2) high cholesterol and high sweet junk food pattern; high in pickles, sweets and condiments, butter and cream, canned fish, visceral meats and eggs; 3) Mediterranean-like pattern; high in vegetables, olive oil, dates, dairy, dried fruits, fruits, red meats, tea and coffee and 4) Ramadan-style pattern; large consumption of Halim, soups, porridges, legumes and whole grains, soft drinks, Zoolbia and Bamieh. Age was positively and inversely associated with Mediterranean-like (P = 0.003; r = 0.17) and Ramadan style (P = 0.1; r = -0.13) dietary pattern, respectively. Pre-Ramadan physical activity level was associated with a Mediterranean-like dietary pattern (P < 0.0001; r = 0.20). CONCLUSIONS: This study showed a Ramadan-specific dietary pattern has unique characteristics, which has not yet been identified as a model of dietary pattern. Also, among identified dietary patterns, Mediterranean-like was the healthiest.

Linear reciprocal interaction between dental caries and salivary characteristics.

Carious tooth surfaces can alter saliva in various ways. We investigated whether salivary parameters were affected by elimination of existing active dental caries in 99 children (age 6-12 years) with more than five carious tooth surfaces. Unstimulated saliva samples were obtained from each child, and salivary characteristics were evaluated. One month after caries treatment that eliminated all existing dental caries, saliva sampling and analysis were repeated. Saliva viscosity significantly decreased (P = 0.04), while pH and saliva buffering capacity significantly increased (P < 0.001). There was no significant difference in unstimulated salivary production at the start of the study and 1 month after caries elimination (P > 0.05). The relationship between dental caries and salivary condition is reciprocal. Treatment of existing dental caries can prevent future caries by improving saliva quality.

The Relationship between Coaches' and Athletes' Competitive Anxiety,and their Performance.

OBJECTIVE: This study was performed to survey the relationship between coaches' and athletes' competitive anxiety, and their performance. METHODS: This is a descriptive correlational study performed using a demographic questionnaire, an athletic performance checklist, and sport competition anxiety test designed by Martens consisting of 15 questions. The study population consisted of 540 players and 60 coaches from 60 futsal teams (5 main players, 4 reserves, and 1 coach for each team). All of the players and the coaches were surveyed in a census method and no sampling was done. The data were analyzed by SPSS software, using chi-square, and Pearson correlation coefficient test. RESULTS: The results showed a positive significant relationship between the coaches' anxiety level and sport competition anxiety level in the athletes (p = 0.019, r = 0.56). It also showed that there was a negative significant relationship between the coaches' anxiety level and performance level of the athletes (p = 0.012, r = -0.80). A negative significant relationship was also demonstrated between the athletes' competitive anxiety level, and their athletic experiences (p < 0.001, r = -0.45) and age (p = 0.001, r = -0.37). CONCLUSIONS: Coaches and officials should consider sport competition anxiety among athletes before and during competitions. Formal and planned competitions, training sessions, and preparation practices can be a major factor assisting to decrease athletes' anxiety. DECLARATION OF INTEREST: None.