Subject(s)
Cryopreservation , Femur Head/cytology , Hematopoietic Stem Cells , Living Donors , Orthopedic Procedures , Tissue Preservation , Aged , Cell Separation/methods , Cell Survival , Cells, Cultured/cytology , Clone Cells/cytology , Colony-Forming Units Assay , Erythroid Precursor Cells/cytology , Female , Granulocytes/cytology , Humans , Macrophages/cytology , Male , Megakaryocytes/cytology , Middle Aged , Specimen Handling/methods , Time Factors , Tissue BanksABSTRACT
BACKGROUND: Collection strategy is the first step for collecting good quality cord blood units. There are two main different techniques for collecting cord blood from the umbilical vein: in the delivery room while the placenta is still in the utero by midwifes and obstetricians, or in an adjacent room after placental delivery by cord blood bank trained personal. Our aim was to evaluate the benefits and disadvantages between the two different cord blood collection strategies in caesarean deliveries. METHODS: We retrospectively analysed data of cord blood units collected from caesarean deliveries for a 3-year period. Caesarean section was performed with a low uterine transversal incision in all patients according to common obstetrical practice. Cord blood collection was performed before or after placental delivery. RESULTS: Obstetrical and umbilical cord blood data was obtained from 253 caesarean deliveries. No statistically significant difference was observed for obstetrical variables or cord blood variables except for Hct and platelets. CONCLUSIONS: We conclude both methods produce comparable TNC, CD34 and CFU counts of cord blood units collected from caesarean sections. Before placental delivery collection avoids the financial investment that generates the presence of cord blood banking personal in the maternity ward.
