[Correlation between MALDI-TOF Vitek-MSTM system and conventional identification methods of gastrointestinal infection causing bacteria]. / Correlación entre el sistema de MALDI-TOF Vitek-MSTM y los métodos convencionales de identificación de bacterias causantes de infección gastrointestinal.

OBJECTIVE: Rapid identification of pathogens is essential for the diagnosis of gastrointestinal infections. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry has shown to be effective and fast for the identification of microorganisms. The objective of this study was to evaluate the correlation between Vitek-MSTM and conventional methods for bacterial identification causing gastrointestinal infection. METHODS: A total of 329 gastrointestinal pathogens were identified using Vitek-MSTM (v2 SARAMIS MS -ID, bioMérieux, Marcy-I´Étoile, France) and routine diagnostic methods simultaneously. In cases of discrepancy 16SrRNA gene sequencing was performed. RESULTS: The correlation between Vitek-MSTM and diagnostic methods was 100% except for Yersinia enterocolitica (94.1%), Helicobacter pylori (10%) and Aeromonas veronii (0 %). CONCLUSIONS: Vitek-MSTM is a quick and useful method for identification of enterophatogenic bacteria. It is necessary to improve the performance of the system for the identification of H. pylori and A. veronii.

Prevención de la infección nosocomial de origen fúngico: verificación de la bioseguridad ambiental en quirófanos / Prevention of nosocomial infection of fungal origin: verification of the environmental biosafety in surgery rooms

Durante 36 meses se ha monitorizado prospectivamente la biodiversidad y la concentración de los hongos ambientales en el área quirúrgica hospitalaria. Se han realizado un total de 120 determinaciones, de las que el 70% presentaron crecimiento fúngico, identificándose en el 62% un único género. Se han aislado 9 géneros diferentes (Penicillium, Aspergillus, Acremonium, Dactylaria, Fusarium, Cladophialophora, Alternaria, Candida y Rodothorula), con un claro predominiode Cladophialophora. Se discuten aspectos aún sin resolver como son: la periodicidad de las tomas, la definición de área de riesgo, la interpretación de los recuentos y las especies consideradas patógenas. Actualmente, a pesar de las grandes lagunas de conocimiento, el mantenimiento de un sistema de registro continuo de los niveles de bioseguridad ambiental es fundamental, ya que en la mayor parte de las ocasiones en las que se detectan recuentos fúngicos elevados no se puede identificar la causa

The biodiversity and concentration of environmental fungi in the hospital surgical area was monitored for 36 months. A total of 120 measures were made, 70 % of which had fungal growth, a single genus being identified in 62 %. Nine different genus were isolated (Penicillium, Aspergillus, Acremonium, Dactylaria, Fusarium, Cladophialophora, Alternaria, Candida and Rodothorula) with a clear predominance of Cladophialophora. Aspects that still must be resolved are discussed. These are: periodicity of the sample obtention, definition of the area of risk, interpretation of the counts and species considered pathogenic. At present, in spite of the large gaps in knowledge, the maintenance of a continuous registry system of the environmental biosafety levels is fundamental, since the cause cannot be identified in most of the times when elevated fungal count is detected

[Prevention of nosocomial infection of fungal origin: verification of the environmental biosafety in surgery rooms]. / Prevención de la infección nosocomial de origen fúngico: verificación de la bioseguridad ambiental en quirófanos.

The biodiversity and concentration of environmental fungi in the hospital surgical area was monitored for 36 months. A total of 120 measures were made, 70% of which had fungal growth, a single genus being identified in 62%. Nine different genus were isolated (Penicillium, Aspergillus, Acremonium, Dactylaria, Fusarium, Cladophialophora, Alternaria, Candida and Rodothorula) with a clear predominance of Cladophialophora. Aspects that still must be resolved are discussed. These are: periodicity of the sample obtention, definition of the area of risk, interpretation of the counts and species considered pathogenic. At present, in spite of the large gaps in knowledge, the maintenance of continuous registry system of the environmental biosafety levels is fundamental, since the cause cannot be identified in most of the times when elevated fungal count is detected.