ABSTRACT
Despite advances in biomedical research, gastric cancer remains the leading cause of morbidity and mortality worldwide due to the limited efficacy of conventional therapies. In recent decades, oncolytic viruses have emerged as a biological therapeutic alternative to cancer due to their selectivity, effectiveness, and low toxicity. However, clinical trials have shown that developing a virus with selectivity for multiple tumor receptors and the ability to penetrate and diffuse through the tumor microenvironment to reactivate the immune system remains challenging. This study aimed to examine the oncolytic potential of tumor cell-adapted rotavirus Wt1-5 in gastric adenocarcinoma samples. This study focused on determining the propagation capacity of the RV Wt1-5 through the tumor and the importance of the expression of cell surface co-receptors, including integrin ß3, protein disulfide isomerase (PDI), and heat shock proteins (Hsp-90, -70, -60, -40, and Hsc 70), during infection of tumor cells. These proteins were found to be differentially expressed in tumor cells compared to adjacent non-tumor cells. Preincubation of gastric tumor cells with antibodies against these proteins decreased rotavirus infections, validating their importance in the binding and entry of RV Wt1-5 into tumor cells, as previously reported. Upon RV infection, apoptosis was one of the types of death that was observed. This was evidenced by evaluating the expression of CASP-3, -9, PARP, cytochrome C, Bax, Bid, p53, and Bcl-2, as well as observing morphological changes such as chromatin margination, nuclear condensation, and fragmentation. Finally, at 60 h.p.i, histological analysis revealed that oncolysis compromised the entire thickness of the tumor. Therefore, the results suggest that RV Wt1-5 could be a novel therapeutic agent co-adjuvant agent for conventional and targeted therapies in managing GC. Ex vivo infection of the tumor tissue model showed characteristics of an immune response that could be explored in future studies.
Subject(s)
Adenocarcinoma , Oncolytic Virotherapy , Oncolytic Viruses , Rotavirus Infections , Rotavirus , Stomach Neoplasms , Humans , Rotavirus/physiology , Stomach Neoplasms/therapy , Oncolytic Viruses/physiology , Adenocarcinoma/therapy , Oncolytic Virotherapy/methods , Tumor MicroenvironmentABSTRACT
This paper aims to validate the hypothesis of an Environmental Kuznets Curve (EKC) for Colombia, introducing the Economic Complexity Index (ECI) into the analysis as a differentiating element of production volumes. We use a Vector Error Correction Model (VECM) to corroborate if there is a long-term relationship between the variables covering the period between 1971-2014. Also, we test the robustness of the results using three different techniques: Dynamic Least Squares, Fully Modified Least Squares, and Canonical Cointegration Regression. The results show that for a developing country like Colombia, the EKC does not exist, and does not yet benefit from increases in economic complexity. Arguably, the country has several hurdles to overcome before achieving the environmental benefits of increased product sophistication. Some of them could be related to the country's own productive and institutional rigidities, which opens the space for public policy intervention.
ABSTRACT
Pulp revascularization is an alternative treatment in immature traumatized teeth with necrotic pulp. However, this procedure has not been reported in horizontal root fractures. This is a case report of a 9-year-old patient with multiple horizontal root fractures in 2 upper central incisors that were successfully treated with pulp revascularization. The patient presented for treatment 2 years after the initial trauma, and revascularization was attempted after the initial treatment with calcium hydroxide had failed. Prior to pulp revascularization, cone-beam computed tomography and autoradiograms demonstrated multiple horizontal fractures in the middle and apical thirds of the roots of the 2 affected teeth. Revascularization was performed in both teeth; platelet-rich plasma (PRP) was used in one tooth (#11) and the conventional method (blood clot) was used in the other tooth (#21). Clinical and radiographic follow-up over 4 years demonstrated pulp calcification in the PRP-treated tooth. Neither of the 2 teeth were lost, and the root canal calcification of tooth #11 was greater than that of tooth #21. This case suggests that PRP-based pulp revascularization may be an alternative for horizontal root fractures.
ABSTRACT
The accumulation of knowledge required to produce economic value is a process that often relates to nations economic growth. Some decades ago many authors, in the absence of other available indicators, used to rely on certain measures of human capital such as years of schooling, enrollment rates, or literacy. In this paper, we show that the predictive power of years of education as a proxy for human capital started to dwindle in 1990 when the schooling of nations began to be homogenized. We developed a structural equation model that estimates a metric of human capital that is less sensitive than average years of education and remains as a significant predictor of economic growth when tested with both cross-section data and panel data.
Subject(s)
Economic Development , Educational Status , Health Status , Socioeconomic Factors , Algorithms , Child, Preschool , Cross-Sectional Studies , Developing Countries , Education , Humans , Infant , Infant, Newborn , Life Expectancy , Models, Economic , Models, Statistical , Mortality , Regression AnalysisABSTRACT
Objetivo: la presente guía se elaboró con el fin de actualizar y complementar la guía demanejo de trauma dentoalveolar de la Fundación Hospital de la Misericordia y la Facultadde Odontología de la Universidad Nacional de Colombia. Métodos: se realizó una búsquedade literatura internacional en diferentes bases de datos y se analizaron los protocolos másrecientes y con mejores resultados en cuanto a la disminución de la aparición y al progresode la reabsorción radicular por remplazo, principal complicación al ser reimplantado undiente permanente avulsionado. Resultados: con esta actualización se pretende sugerir lamodificación de la terapia medicamentosa intraconducto y el acondicionamiento radicular,al encontrarse mejores mecanismos de acción de los medicamentos utilizados actualmente.Se recomienda llevar a cabo una socialización y aplicación de esta guía de manejo dentro dela Facultad de Odontología de la Universidad Nacional de Colombia, como en el Servicio deSalud Oral de la Fundación Hospital de la Misericordia...
Objective: This guide was conducted to update and complement the current managementguide for dentoalveolar trauma at the La Misericordia Hospital Foundation and the NationalUniversity of Colombia Dental School. Methods: A search of international literature in differentdatabases was carried out as well as the analysis of the most recent protocols andwith better results for decreasing the occurrence of root resorption, the main complicationafter an avulsed permanent teeth has been replanted. Results: This update is intended tosuggest the modification of the intracanal drug therapy and root surface conditioning to findbetter mechanisms of action of drugs currently used. It is recommended to disseminate andimplement this guide at the National University of Colombia Dental School and the dentalcare unit of the La Misericordia Hospital Foundation...
Subject(s)
Alveolar Process/injuries , Replantation , Root ResorptionABSTRACT
El internista venezolano esta trabajando en la definición de su área de trabajo en este grupo poblacional. Conocer los factores de riesgo en general del adolescente. Estudio transversal, multicéntrico de adolescentes que asisten a consulta de medicina interna.Procedimiento: 1) Autorización escrita, 2) Explicación de los objetivos, 3) Encuesta de factores de riesgo, 4) Diagnósticos. El análisis fue descriptivo y comparativo. 75 individuos estudiados; 69 por ciento mujeres. 11 por ciento tenían alteración de la figura paterna; el 27 por ciento habían sufrido repitencia escolar; 64 por ciento no comparte con la familia; 43 por ciento inició la ingesta alcohólica con amigos. La violencia fue: 39 por ciento en el hogar y 55 por ciento fuera del hogar; 43 por ciento eran sexualmente activos; 28 por ciento de las mujeres tenían historia de embarazos, 1 de cada 12 había sido abusado sexualmente; 7 reconocieron el uso de drogas. Se definen áreas de trabajo: la comunicación familiar, educación sexual, historia de abuso sexual, educación antiviolencia y educación preventiva general. Se propone utilizar los resultados del presente estudio para incluir en la historia clínica diaria en forma permanente áreas de orientación para los adolescentes y sus padres
Subject(s)
Humans , Male , Female , Adolescent , Adolescent , Avoidance Learning , Case Management , Evaluation Study , Health Status , Violence , MedicineABSTRACT
Se estudiaron 12 pacientes con diagnóstico del nistagmo y posición compensadora de la cabeza (PCC) asociado o no a estrabismo. Todos presentaron PCC en los tres ejes: horizontal, vertical y sagital, con predominio del componente rotacional. A todos ellos se les realizó cirugía de Anderson: debilitamiento amplio de los rectos horizontales responsables de la posición del bloqueo, más cirugía horizontal. Se concluye que en los pacientes con nistagmo y PCC con franco predominio de la rotación es innecesario realizar cirugía sobre los músculos verticales.
Subject(s)
Humans , Male , Female , Child, Preschool , Nystagmus, Pathologic/surgery , Oculomotor Muscles/surgery , Oculomotor Muscles/physiopathology , Strabismus/surgeryABSTRACT
Describimos un caso poco frecuente de cardiopatía congénita del tipo de la estenosis valvular pulmonar con septum ventricular íntegro que repercutió de manera funcional sobre la válvula tricuspídea, se mencionan los métodos diagnósticos y terapéuticos, tanto los procedimientos no quirúrgicos como es el caso de la valvulotomía con catéter de balón en el laboratorio de hemodinamia si como la solución quirúrgica de ambas válvulas
