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1.
Arch Med Res ; 47(2): 142-5, 2016 02.
Article in English | MEDLINE | ID: mdl-27133711

ABSTRACT

Glutathione S-transferases (GSTs) are a group of phase II detoxification enzymes, which catalyze the conjugation of glutathione (GSH) with carcinogens, among other xenobiotics. The GSTM3 gene is part of the GSTs gene family, and its polymorphism A/B has been associated with risk and protective effects of several cancers. This genetic variant is a deletion of 3 bp (AGG) in intron 6. Previous association studies have performed genotyping using techniques such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In this study, we took advantage of the TaqMan(®) probes features and developed a reliable, faster, more simple and economic method to identify the 3-bp deletion. Our allelic discrimination method was able to distinguish between homozygous A/A, heterozygous A/B and homozygous B/B samples, as shown by TaqMan(®) based real-time PCR. Results were validated by Sanger Sequencing. In conclusion, we developed a specific and rapid method to detect the 3-bp deletion from the GSTM3 A/B polymorphism.


Subject(s)
Glutathione Transferase/genetics , Alleles , Amplified Fragment Length Polymorphism Analysis , DNA Probes , Genotype , Heterozygote , Homozygote , Humans , Introns , Isoenzymes/genetics , Polymorphism, Genetic , Real-Time Polymerase Chain Reaction , Sequence Deletion
2.
Mycopathologia ; 179(1-2): 153-7, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25344197

ABSTRACT

Chromoblastomycosis is a subcutaneous, chronic, granulomatous mycosis that occurs more frequently in tropical and subtropical countries. We describe a case of a 63-year-old male patient with diagnosis of chromoblastomycosis by Cladophialophora carrionii with an 18-year evolution who developed a lethal squamous cell carcinoma.


Subject(s)
Antifungal Agents/therapeutic use , Ascomycota/genetics , Chromoblastomycosis/diagnosis , Chromoblastomycosis/drug therapy , DNA, Ribosomal Spacer/genetics , Ascomycota/drug effects , Ascomycota/isolation & purification , Base Sequence , Carcinoma, Squamous Cell , Chromoblastomycosis/microbiology , DNA, Fungal/genetics , Humans , Male , Middle Aged , Molecular Sequence Data , Opportunistic Infections/diagnosis , Opportunistic Infections/drug therapy , Opportunistic Infections/microbiology , Sequence Analysis, DNA , Treatment Outcome
3.
Med Mycol ; 51(2): 170-7, 2013 Feb.
Article in English | MEDLINE | ID: mdl-22734968

ABSTRACT

We identified 29 Cladophialophora carrionii isolates recovered from Venezuelan patients with chromoblastomycosis using phenotypic and molecular characteristics. The genetic diversity of isolates was assessed by enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) fingerprinting. We detected four electrophoretic patterns divided into two main clusters (I and II) comprising 10 and 17 isolates, respectively, and two minor clusters (III and IV) with one isolate each. An interesting cluster-age-lesion type association was detected. The median age of patients in cluster I was 37.5 years and in cluster II, 55 years of age (P = 0.04). The C. carrionii isolates found in cluster I were generally obtained from crusty lesions (60%) and isolates in cluster II were usually recovered from plaque type lesions (53%) even though the P values were only slightly less than significant (P = 0.08). No associations were found among the genetic features strains in the two clusters and gender, occupation, geographic origin, lesion size, severity, and duration of the disease. There was also no correlation between antifungal susceptibilities and strain clustering. In conclusion, molecular typing using ERIC-PCR revealed a genomic heterogeneity in the C. carrionii clinical isolates studied.


Subject(s)
Antifungal Agents/pharmacology , Ascomycota/genetics , Chromoblastomycosis/microbiology , Genetic Variation , Adolescent , Adult , Aged , Aged, 80 and over , Ascomycota/drug effects , Ascomycota/isolation & purification , Ascomycota/physiology , Base Sequence , Child , Cluster Analysis , DNA, Fungal/chemistry , DNA, Fungal/genetics , DNA, Ribosomal Spacer/chemistry , DNA, Ribosomal Spacer/genetics , Demography , Female , Humans , Male , Microbial Sensitivity Tests , Middle Aged , Molecular Sequence Data , Repetitive Sequences, Nucleic Acid , Sequence Analysis, DNA , Venezuela , Young Adult
4.
Med Mycol Case Rep ; 2: 148-51, 2013 Aug 31.
Article in English | MEDLINE | ID: mdl-24432242

ABSTRACT

A case of chromoblastomycosis of the hand caused by Rhinocladiella aquaspersa is described. The case was acquired locally in tropical Venezuela and was successfully treated with oral itraconazole.

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