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1.
Molecules ; 29(9)2024 Apr 25.
Article in English | MEDLINE | ID: mdl-38731469

ABSTRACT

The growing demand for agricultural products has increased exponentially, causing their waste to increase and become a problem for society. Searching for sustainable solutions for organic waste management is increasingly urgent. This research focuses on considering the waste of an Andean tuber, such as Olluco, as a fuel source for generating electricity and becoming a potential sustainable energy source for companies dedicated to this area. This research used Olluco waste as fuel in single-chamber microbial fuel cells using carbon and zinc electrodes. An electric current and electric potential of 6.4 ± 0.4 mA and 0.99 ± 0.09 V were generated, operating with an electrical conductivity of 142.3 ± 6.1 mS/cm and a pH of 7.1 ± 0.2. It was possible to obtain a 94% decrease in COD and an internal resistance of 24.9 ± 2.8 Ω. The power density found was 373.8 ± 28.8 mW/cm2 and the current density was 4.96 A/cm2. On day 14, the cells were connected in earnest, achieving a power of 2.92 V and generating enough current to light an LED light bulb, thus demonstrating the potential that Olluco waste has to be used as fuel in microbial fuel cells.

2.
Microorganisms ; 11(5)2023 Apr 29.
Article in English | MEDLINE | ID: mdl-37317142

ABSTRACT

The effluents generated in the paper industry, such as black liquor, have a high content of lignin and other toxic components; however, they represent a source of lignin-degrading bacteria with biotechnological potential. Therefore, the present study aimed to isolate and identify lignin-degrading bacteria species in paper mill sludge. A primary isolation was carried out from samples of sludge present in environments around a paper company located in the province of Ascope (Peru). Bacteria selection was made by the degradation of Lignin Kraft as the only carbon source in a solid medium. Finally, the laccase activity (Um-L-1) of each selected bacteria was determined by oxidation of 2,2'-azinobis-(3-etilbencenotiazolina-6-sulfonate) (ABTS). Bacterial species with laccase activity were identified by molecular biology techniques. Seven species of bacteria with laccase activity and the ability to degrade lignin were identified. The bacteria Agrobacterium tumefasciens (2), Klebsiella grimontii (1), and Beijeinckia fluminensis (1) were reported for first time. K. grimowntii and B. fluminensis presented the highest laccase activity, with values of 0.319 ± 0.005 UmL-1 and 0.329 ± 0.004 UmL-1, respectively. In conclusion, paper mill sludge may represent a source of lignin-degrading bacteria with laccase activity, and they could have potential biotechnological applications.

3.
Molecules ; 27(21)2022 Oct 31.
Article in English | MEDLINE | ID: mdl-36364216

ABSTRACT

The excessive use of fossil sources for the generation of electrical energy and the increase in different organic wastes have caused great damage to the environment; these problems have promoted new ways of generating electricity in an eco-friendly manner using organic waste. In this sense, this research uses single-chamber microbial fuel cells with zinc and copper as electrodes and pineapple waste as fuel (substrate). Current and voltage peaks of 4.95667 ± 0.54775 mA and 0.99 ± 0.03 V were generated on days 16 and 20, respectively, with the substrate operating at an acid pH of 5.21 ± 0.18 and an electrical conductivity of 145.16 ± 9.86 mS/cm at two degrees Brix. Thus, it was also found that the internal resistance of the cells was 865.845 ± 4.726 Ω, and a maximum power density of 513.99 ± 6.54 mW/m2 was generated at a current density of 6.123 A/m2, and the final FTIR spectrum showed a clear decrease in the initial transmittance peaks. Finally, from the biofilm formed on the anodic electrode, it was possible to molecularly identify the yeast Wickerhamomyces anomalus with 99.82% accuracy. In this way, this research provides a method that companies exporting and importing this fruit may use to generate electrical energy from its waste.


Subject(s)
Ananas , Bioelectric Energy Sources , Electricity , Electrodes , Biofilms , Wastewater
4.
Article in English | MEDLINE | ID: mdl-36293682

ABSTRACT

There are several techniques for the removal of pharmaceuticals (drugs) from wastewater; however, strengths and weaknesses have been observed in their elimination processes that limit their applicability. Therefore, we aimed to evaluate the best techniques for the removal of pharmaceuticals from municipal and hospital wastewater. For this, a non-experimental, descriptive, qualitative-quantitative design was used, corresponding to a systematic review without meta-analysis. Based on established inclusion and exclusion criteria, 31 open-access articles were selected from the Scopus, ProQuest, EBSCOhost, and ScienceDirect databases. The results showed that high concentrations of analgesics such as naproxen (1.37 mg/L) and antibiotics such as norfloxacin (0.561 mg/L) are frequently found in wastewater and that techniques such as reverse osmosis, ozonation, and activated sludge have the best removal efficiency, achieving values of 99%. It was concluded that reverse osmosis is one of the most efficient techniques for eliminating ofloxacin, sulfamethoxazole, carbamazepine, and diclofenac from municipal wastewater, with removal rates ranging from 96 to 99.9%, while for hospital wastewater the activated sludge technique proved to be efficient, eliminating analgesics and antibiotics in the range of 41-99%.


Subject(s)
Ozone , Water Pollutants, Chemical , Wastewater/analysis , Sewage , Diclofenac , Naproxen/analysis , Norfloxacin , Water Pollutants, Chemical/analysis , Carbamazepine/analysis , Hospitals , Ozone/analysis , Sulfamethoxazole , Anti-Bacterial Agents , Ofloxacin , Pharmaceutical Preparations , Waste Disposal, Fluid/methods
5.
Molecules ; 27(16)2022 Aug 15.
Article in English | MEDLINE | ID: mdl-36014437

ABSTRACT

The accelerated increase in energy consumption by human activity has generated an increase in the search for new energies that do not pollute the environment, due to this, microbial fuel cells are shown as a promising technology. The objective of this research was to observe the influence on the generation of bioelectricity of sucrose, with different percentages (0%, 5%, 10% and 20%), in papaya waste using microbial fuel cells (MFCs). It was possible to generate voltage and current peaks of 0.955 V and 5.079 mA for the cell with 20% sucrose, which operated at an optimal pH of 4.98 on day fifteen. In the same way, the internal resistance values of all the cells were influenced by the increase in sucrose, showing that the cell without sucrose was 0.1952 ± 0.00214 KΩ and with 20% it was 0.044306 ± 0.0014 KΩ. The maximum power density was 583.09 mW/cm2 at a current density of 407.13 A/cm2 and with a peak voltage of 910.94 mV, while phenolic compounds are the ones with the greatest presence in the FTIR (Fourier transform infrared spectroscopy) absorbance spectrum. We were able to molecularly identify the species Achromobacter xylosoxidans (99.32%), Acinetobacter bereziniae (99.93%) and Stenotrophomonas maltophilia (100%) present in the anode electrode of the MFCs. This research gives a novel use for sucrose to increase the energy values in a microbial fuel cell, improving the existing ones and generating a novel way of generating electricity that is friendly to the environment.


Subject(s)
Bioelectric Energy Sources , Carica , Electricity , Electrodes , Humans , Sucrose
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