Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters










Database
Language
Publication year range
1.
J Agric Food Chem ; 58(9): 5308-14, 2010 May 12.
Article in English | MEDLINE | ID: mdl-20356042

ABSTRACT

Onion may contribute to the health effects associated with high fruit and vegetable consumption. A considerable amount of onion production ends up as waste that might find use in foods. Onion byproduct has not yet been explored for potential health benefits. The aim of this study is to elucidate the safety and potential role of onion byproducts in affecting risk markers of cardiovascular disease (CVD). For that purpose, the effects of an onion byproduct, Allium cepa L. cepa 'Recas' (OBP), and its two derived fractions, an ethanolic extract (OE) and a residue (OR), on the distribution of plasma lipids and on factors affecting cholesterol metabolism in healthy rats have been investigated. The OBP or its fractions did not significantly reduce cholesterol or down-regulate hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase (Hmgcr) gene expression. The OR even had the effect of increasing plasma triacylglycerides (TAG) and cholesterol in the very low density lipoprotein (VLDL-C) fraction. Neither total bile acids nor total primary or secondary bile acids were significantly affected by feeding rats the OBP or its fractions. Principal component analysis combining all markers revealed that the controls could be completely separated from OBP, OE, and OR groups in the scores plot and also that OE and OR groups were separated. Plasma lipids and bile acid excretion were the discriminating loading factors for separating OE and OR but also contributed to the separation of onion-fed animals and controls. It was concluded that the onion byproduct did not present significant beneficial effects on individual markers related to plasma lipid transport in this healthy rat model but that onion byproduct contains factors with the ability to modulate plasma lipids and lipoprotein levels.


Subject(s)
Lipids/blood , Onions , Animals , Chromatography, Liquid , Feces , Magnetic Resonance Spectroscopy , Male , Polymerase Chain Reaction , Principal Component Analysis , Rats , Rats, Inbred F344 , Tandem Mass Spectrometry
2.
Analyst ; 134(11): 2344-51, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19838425

ABSTRACT

The metabolome following intake of onion by-products is evaluated. Thirty-two rats were fed a diet containing an onion by-product or one of the two derived onion by-product fractions: an ethanol extract and the residue. A 24 hour urine sample was analyzed using (1)H NMR spectroscopy in order to investigate the effects of onion intake on the rat metabolism. Application of interval extended canonical variates analysis (ECVA) proved to be able to distinguish between the metabolomic profiles from rats consuming normal feed and rats fed with an onion diet. Two dietary biomarkers for onion intake were identified as dimethyl sulfone and 3-hydroxyphenylacetic acid. The same two dietary biomarkers were subsequently revealed by interval partial least squares regression (PLS) to be perfect quantitative markers for onion intake. The best PLS calibration model yielded a root mean square error of cross-validation (RMSECV) of 0.97% (w/w) with only 1 latent variable and a squared correlation coefficient of 0.94. This indicates that urine from rats on the by-product diet, the extract diet, and the residue diet all contain the same dietary biomarkers and it is concluded that dimethyl sulfone and 3-hydroxyphenylacetic acid are dietary biomarkers for onion intake. Being able to detect specific dietary biomarkers is highly beneficial in the control of nutritionally enhanced functional foods.


Subject(s)
Diet , Dimethyl Sulfoxide/metabolism , Eating , Metabolomics , Nutritional Physiological Phenomena , Onions , Sulfones/metabolism , Animals , Antioxidants/chemistry , Antioxidants/metabolism , Antioxidants/pharmacology , Biomarkers/chemistry , Biomarkers/metabolism , Biomarkers/urine , Dimethyl Sulfoxide/chemistry , Dimethyl Sulfoxide/urine , Ethanol/chemistry , Magnetic Resonance Spectroscopy , Male , Onions/chemistry , Phenylacetates/chemistry , Phenylacetates/metabolism , Phenylacetates/urine , Plant Extracts/chemistry , Plant Extracts/metabolism , Plant Extracts/pharmacology , Plant Extracts/urine , Principal Component Analysis , Rats , Solubility , Sulfones/chemistry , Sulfones/urine
3.
Br J Nutr ; 102(11): 1574-82, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19682402

ABSTRACT

Onions are excellent sources of bioactive compounds including fructo-oligosaccharides (FOS) and polyphenols. An onion by-product was characterised in order to be developed as a potentially bioactive food ingredient. Our main aim was to investigate whether the potential health and safety effects of this onion by-product were shared by either of two derived fractions, an extract containing the onion FOS and polyphenols and a residue fraction containing mainly cell wall materials. We report here on the effects of feeding these products on markers of potential toxicity, protective enzymes and gut environment in healthy rats. Rats were fed during 4 weeks with a diet containing the products or a control feed balanced in carbohydrate. The onion by-product and the extract caused anaemia as expected in rodents for Allium products. No other toxicity was observed, including genotoxicity. Glutathione reductase (GR) and glutathione peroxidase (GPx1) activities in erythrocytes increased when rats were fed with the onion extract. Hepatic gene expression of Gr, Gpx1, catalase, 5-aminolevulinate synthase and NAD(P)H:quinone oxidoreductase was not altered in any group of the onion fed rats. By contrast, gamma-glutamate cysteine ligase catalytic subunit gene expression was upregulated but only in rats given the onion residue. The onion by-products as well as the soluble and insoluble fractions had prebiotic effects as evidenced by decreased pH, increased butyrate production and altered gut microbiota enzyme activities. In conclusion, the onion by-products have no in vivo genotoxicity, may support in vivo antioxidative defence and alter the functionality of the rat gut microbiota.


Subject(s)
Cecum/microbiology , DNA Damage , Onions/chemistry , Plant Extracts/adverse effects , Animals , Antioxidants/metabolism , Cecum/anatomy & histology , Dietary Carbohydrates/analysis , Fatty Acids, Volatile/biosynthesis , Food Analysis/methods , Fructans/analysis , Gastrointestinal Transit/drug effects , Gene Expression Regulation, Enzymologic/drug effects , Heme/biosynthesis , Hemoglobins/metabolism , Hydrogen-Ion Concentration/drug effects , Liver/enzymology , Male , Models, Animal , Oligosaccharides/analysis , Organ Size/drug effects , Plant Extracts/pharmacology , Rats , Rats, Inbred F344
SELECTION OF CITATIONS
SEARCH DETAIL
...