ABSTRACT
The method of determination of parasitic bacteria Bdellovibrio sensitivity to pteridine has been described. The method suggested can be used in research work for diagnostics of Bdellovibrio genus bacteria.
Subject(s)
Bdellovibrio/drug effects , Pteridines/pharmacology , Microbial Sensitivity Tests/methods , Time FactorsABSTRACT
Micavibrio gen. nov. (M.L. fem. n. mica--a tiny thing and M.L. masc. n. Vibroia generic name, M.L. masc. n. Micavibrio a tiny vibrio). Cells are single, small curved rods, in motile stage with a single polar not sheathed flagellum. Reproduced by binary division, gram-negative. Cells attach to host-bacteria cells and grow as exoparasites destroying the host, may exhibit a host-range specificity. The G + C content of the DNA of the strain examined is 57,1% M. Isolated from sewage waters. The type species is M. admirandus sp. nov. M. admirandus sp. nov (M.L. adj. admirable). Curved gram-negative rods 0,25-0,35X0,6-1,0 micron with a single flagellum 13-15 nm in width. Motile cells attach to the cell wall of the bacterium. Pseudomonas maltophilia, lose the motility and grow as exoparasites destroying the host. Develops only on. P. maltophilia cells and is unable to multiply in the absence of a host organism. Resistant to a vibriostatic agent pteridine 0/129. The G + C content of the DNA of the strain examined is 57,1% M. The type strain ARL-14 is maintained in the culture collection of the Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences. The type strain has been isolated from sewage waters of the town of Pushchino.
Subject(s)
Bacteria/classification , Bacteria/ultrastructure , Microscopy, Electron , Pseudomonas , Sewage , USSRABSTRACT
The morphology, the host ranges, the resistance to pteridine and the nucleotide composition of DNA were compared in 12 newly isolated and 10 collection strains of Bdellovibrio. The significance of properties used for the taxonomy of these organisms was evaluated. The host ranges of Bdellovibrio strains are heterogeneous with respect to the taxonomy of host bacteria. The specificity of the parasite depends to a significant degree on the host bacterium in which it grows. All the strains including Bd. starrii which was described earlier as a pteridine resistant species are sensitive to pteridine. Therefore, such properties as the host range action and the response to pteridine cannot be used for diagnostics of Bdellovibrio species. The strains were found to be very heterogeneous with regard to the nucleotide composition of the DNA. Eight out of the 12 newly isolated strains were assigned to the species Bd, bacteriovorus.