ABSTRACT
The aim of this study was to evaluate the occurrence of genital mycoplasmas, especially Ureaplasma parvum and Ureaplasma urealyticum, in women with atypical squamous cells of undetermined significance (ASCUS), low grade squamous intraepithelial lesions (LSIL) and high grade squamous intraepithelial lesions (HSIL), compared to women with normal cytology living in Katowice, Poland. Two sterile swabs were used to obtain material from the posterior vaginal fornix of 143 women with squamous intraepithelial lesions and 39 healthy women: first for general bacteriology, second for detection of urogenital mycoplasmas using Mycoplasma IST2 kit. From each positive Mycoplasma IST2 culture DNA was isolated and PCR was performed for identification of U. parvum and U. urealyticum. Mycoplasma IST was positive in 34.1% cases. Urogenital mycoplasmas were demonstrated in women with HSIL significantly more often compared to women with LSIL, ASCUS, and with normal cytology. DNA of U. parvum was demonstrated in majority of Mycoplasma IST2-positive cases, U. urealyticum DNA-only in 9 (4.9%). Predominance of 3/14 serovars of U. parvum was demonstrated. U. urealyticum biovar 2 was present more often in women with squamous intraepithelial lesions.
Subject(s)
Ureaplasma Infections/microbiology , Ureaplasma urealyticum/genetics , Ureaplasma/genetics , Uterine Cervical Dysplasia/microbiology , Adult , Animals , Female , Humans , Male , Poland/epidemiology , Pregnancy , Ureaplasma Infections/epidemiology , Uterine Cervical Dysplasia/pathologyABSTRACT
Development of female genito-urinary infections depends on many factors, such as immune system activity, virulence of microorganism and production of factors inhibiting the growth of microorganisms. Taking into account the possibility of relapses or severe complications, it is very important to appropriately diagnose and treat infections. Because of recently observed increase of microbial resistance to antibiotics, researchers are looking for alternatives. In our study we evaluated and compared the concentration of human neutrophil peptides (HNP 1-3) in cervico-vaginal lavages (CVL), obtained from women with vaginitis/cervicitis. Swabs from the posterior vaginal fornix and from the endocervical canal as well as CVL samples were obtained from 32 patients with vaginitis/cervicitis and 29 healthy women (control group). Supernatants of CVL were used for determination of concentration of HNP by ELISA. The difference between concentrations of HNP 1-3 in studied and control groups was statistically significant (p = 0.018). The maximal concentration was determined in patients with mixed infections (28.41 ng/ml), and Group B Streptococci, GBS, (28.06 ng/ml), the minimal concentrations in cases of C. trachomatis (mean concentrations did not differ from those in the control group: 16.93 ng/ml and 16.39 ng/ml, respectively). Maximal correlation was determined for control-studied group with isolation of GBS (r = 0.79), and very high negative correlation for group of GBS - C. trachomatis (r = -0.98).
Subject(s)
Uterine Cervicitis/metabolism , Uterine Cervicitis/microbiology , Vaginitis/metabolism , Vaginitis/microbiology , alpha-Defensins/metabolism , Adult , Anti-Infective Agents , Candida albicans , Candidiasis/metabolism , Chlamydia Infections/metabolism , Chlamydia trachomatis , Female , Humans , Streptococcal Infections/metabolism , Streptococcus agalactiae/isolation & purification , Ureaplasma Infections/metabolism , Ureaplasma urealyticumABSTRACT
The changes in vaginal ecosystem (decreasing number of H2O2 producing Lactobacillus spp. and increasing number of Mobiluncus spp., Gardnerella vaginalis, Atopobium vaginae, Prevotella spp., Mycoplasma hominis, Peptostreptococcus spp.) appears to be a major cause of bacterial vaginosis (BV). Bacterial vaginosis is associated with infectious complications in pregnant and non-pregnant women and increasing risk of STI. The preventive role of natural vaginal microflora and problems of BV treatment are discussed in the light of the recent publications.
