Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
BMC Res Notes ; 11(1): 635, 2018 Sep 03.
Article in English | MEDLINE | ID: mdl-30176909

ABSTRACT

BACKGROUND: Diabetes mellitus patients (DM) have more severe progression of atherosclerotic disease than non-diabetic (NDM) individuals. In situ inflammation and oxidative stress are key points in the pathophysiology of atherosclerosis, a concept largely based on animal model research. There are few studies comparing inflammation and oxidative stress parameters in medium-sized arteries between DM and NDM patients. A fragment of the internal mammary artery used in coronary artery bypass grafting (CABG) will be employed for this purpose OBJECTIVE: To assess the expression of inflammatory markers tumor necrosis factor-α, transforming growth factor-ß1, nuclear factor kappa B, the enzymes superoxide dismutase, and catalase in the vascular wall of the arterial graft used in CABG, comparing DM and NDM patients RESULTS: The present study will add information to the vascular degenerative processes occurring in diabetic patients.


Subject(s)
Coronary Artery Bypass , Coronary Artery Disease/immunology , Inflammation , Oxidative Stress , Adolescent , Adult , Coronary Artery Disease/physiopathology , Cross-Sectional Studies , Diabetes Complications , Diabetes Mellitus , Humans , Male , Research Design
2.
Mutat Res ; 631(1): 44-54, 2007 Jul 10.
Article in English | MEDLINE | ID: mdl-17507284

ABSTRACT

Diphenyl diselenide (DPDS) is an electrophilic reagent used in the synthesis of a variety of pharmacologically active organic selenium compounds. Studies have shown its antioxidant, hepatoprotective, neuroprotective, anti-inflammatory, and antinociceptive effects. We recently showed the antioxidant effect of DPDS in V79 cells, and established the beneficial and toxic doses of this compound in this cell line. Here, we report the antigenotoxic and antimutagenic properties of DPDS, investigated by using a permanent lung fibroblast cell line derived from Chinese hamsters. We determined the cytotoxicity by clonal survival assay, and evaluated DNA damage in response to several mutagens by comet assay and micronucleus test in binucleated cells. In the clonal survival assay, at concentrations ranging from 1.62 to 12.5microM, DPDS was not cytotoxic, while at concentrations up to 25microM, it significantly decreased survival. The treatment with this organoselenium compound at non-cytotoxic dose range increased cell survival after challenge with hydrogen peroxide, methyl-methanesulphonate, and UVC radiation, but did not protect against 8-methoxypsoralen plus UVA-induced cytotoxicity. In addition, the treatment prevented induced DNA damage, as verified in the comet assay. The mutagenic effect of these genotoxins, as measured by the micronucleus test, similarly attenuated or prevented cytotoxicity and DNA damage. Treatment with DPDS also decreased lipid peroxidation levels after exposure to hydrogen peroxide MMS, and UVC radiation, and increased glutathione peroxidase activity in the extracts. Our results clearly demonstrate that DPDS at low concentrations presents antimutagenic properties, which are most probably due to its antioxidant properties.


Subject(s)
Antimutagenic Agents/pharmacology , Antioxidants/pharmacology , Benzene Derivatives/pharmacology , Mutagens/toxicity , Organoselenium Compounds/pharmacology , Animals , Catalase/metabolism , Cell Line , Cell Survival/drug effects , Comet Assay , Cricetinae , Cricetulus , DNA Damage , Glutathione Peroxidase/metabolism , Hydrogen Peroxide/toxicity , Lipid Peroxidation/drug effects , Methyl Methanesulfonate/toxicity , Micronucleus Tests , Mutagenicity Tests , PUVA Therapy/adverse effects , Superoxide Dismutase/metabolism , Ultraviolet Rays/adverse effects
SELECTION OF CITATIONS
SEARCH DETAIL
...